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Dades primàries associades a un article publicat a la revista PeerJ https://doi.org/10.7717/peerj.5440

Dades primàries associades a un article publicat a la revista PeerJ disponible a l'adreça http://dx.doi.org/10.7717/peerj.2725, Podeu consultar l'article a: http://hdl.handle.net/2445/104686, Background. White adipose tissue (WAT) is a complex, diffuse, multifunctional organ which contains adipocytes, and a large proportion of fat, but also other cell types, active in defence, regeneration and signalling functions. Studies with adipocytes often require their isolation from WAT by breaking up the matrix of collagen fibres, however, it is unclear to what extent adipocyte number in primary cultures correlates with their number in intact WAT, since recovery and viability are often unknown. Experimental design. Epididymal WAT of 4-6 young adult rats was used to isolate adipocytes with collagenase. Careful recording of lipid content of tissue, and all fraction volumes and weights, allowed us to trace the amount of initial WAT fat remaining in the cell preparation. Functionality was estimated by incubation with glucose and measurement of lactate, glycerol and NEFA production. Non-adipocyte cells were also recovered and their sizes (and those of adipocytes) were also measured. The presence of non-nucleated cells (erythrocytes) was also estimated. Results. Cell numbers and sizes were correlated from all fractions to intact WAT. Tracing the lipid content, the recovery of adipocytes in the final, metabolically active, preparation was in the range of 70-75%. Adipocytes were 7%, erythrocytes 68% and other stromal (nucleated cells) 24% of total WAT cells. However, their overall volumes were, 91%, 0.05%, and 0.2% of WAT. Non-fat volume of adipocytes was 2.5% of WAT. Conclusions. The methodology presented here allows for a direct quantitative reference to the original tissue of studies using isolated cells. We have found, also, that the "live cell mass" of adipose tissue is very small (about 25 µL/g for adipocytes and 2 µL/g stromal, plus about 1 µL/g blood). This fact, translates (with respect to the actual "live cytoplasm" size) into an extremely high metabolic activity, which make WAT an even more significant agent in the control of energy metabolism.

There are twenty zip files each one corresponding to a participant. The acronym PT stands for patients (from 01 to 10) and HC stands for healthy controls (from 01 to 10). Once unzipped, structural and functional data of each patient and healthy control are located in the folder indicated by the specific acronym and number of the participant (i.e. PT01). Thus, each folder contains the original functional data of the resting state sequence (fmri.nii.gz), the preprocessed functional data (fmri_res.nii.gz), the original structural T1-weighted image (T1.nii.gz) and the structural T1-weighted image after applying the brain extraction with the Brain Extraction Tool of fsl (T1_brain.nii.gz) and used to perform the co-registration with the functional data. All images are in NIfTI (Neuroimaging Computer Technology Initiative) format, as a gzip compressed file, normally used by different software as the software library FMRIB (FSL). We also provide the NIfTI file of the AAL Atlas used to parcellate the brain during the analysis (AAL_Contract_90_3MM.nii) and the corresponding text file indicating the list of regions composing the atlas (AAL_LabelID_90.txt)., Podeu consultar el document publicat a: http://hdl.handle.net/2445/161359, Dades primàries associades a un article publicat a la revista PLoS One, 2020, vol. 15, num. 1, p. e0226816 [https://doi.org/10.1371/journal.pone.0226816], We tested 10 hemianopic patients and 10 healthy participants. They were scanned in a 1.5 Tesla Philips scanner at the Borgo Roma Hospital in Verona (Italy). We collected a whole brain high-resolution (1x1x1 mm3) 3D T1-weighted image with magnetization-prepared rapid acquisition gradient echo (MPRAGE) and one hundred sixty resting state volumes with TR=2500ms, Echo Time=50ms; 30 slices with slice thickness of 4mm, Field of View = 224x224mm, time duration = 6.47m and echo train length = 39. The entire sample was were scanned during the period of time going from February 2017 until July 2018.