BUSCADOR RECOLECTA

Maitotoxins (MTXs) are among the most potent toxins known. These toxins are produced by epi-benthic dinoflagellates of the genera Gambierdiscus and Fukuyoa and may play a role in causing the symptoms associated with Ciguatera Fish Poisoning. A recent survey revealed that, of the species tested, the newly described species from the Canary Islands, G. excentricus, is one of the most maitotoxic. The goal of the present study was to characterize MTX-related compounds produced by this species. Initially, lysates of cells from two Canary Island G. excentricus strains VGO791 and VGO792 were partially purified by (i) liquid-liquid partitioning between dichloromethane and aqueous methanol followed by (ii) size-exclusion chromatography. Fractions from chromatographic separation were screened for MTX toxicity using both the neuroblastoma neuro-2a (N2a) cytotoxicity and Ca2+ flux functional assays. Fractions containing MTX activity were analyzed using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) to pinpoint potential MTX analogs. Subsequent non-targeted HRMS analysis permitted the identification of a novel MTX analog, maitotoxin-4 (MTX4, accurate mono-isotopic mass of 3292.4860 Da, as free acid form) in the most toxic fractions. HRMS/MS spectra of MTX4 as well as of MTX are presented. In addition, crude methanolic extracts of five other strains of G. excentricus and 37 other strains representing one Fukuyoa species and ten species, one ribotype and one undetermined strain/species of Gambierdiscus were screened for the presence of MTXs using low resolution tandem mass spectrometry (LRMS/MS). This targeted analysis indicated the original maitotoxin (MTX) was only present in one strain (G. australes S080911_1). Putative maitotoxin-2 (p-MTX2) and maitotoxin-3 (p-MTX3) were identified in several other species, but confirmation was not possible because of the lack of reference material. Maitotoxin-4 was detected in all seven strains of G. excentricus examined, independently of their origin (Brazil, Canary Islands and Caribbean), and not detected in any other species. MTX4 may therefore serve as a biomarker for the highly toxic G. excentricus in the Atlantic area.

Maitotoxins (MTXs) are among the most potent toxins known. These toxins are produced by epi-benthic dinoflagellates of the genera Gambierdiscus and Fukuyoa and may play a role in causing the symptoms associated with Ciguatera Fish Poisoning. A recent survey revealed that, of the species tested, the newly described species from the Canary Islands, G. excentricus, is one of the most maitotoxic. The goal of the present study was to characterize MTX-related compounds produced by this species. Initially, lysates of cells from two Canary Island G. excentricus strains VGO791 and VGO792 were partially purified by (i) liquid-liquid partitioning between dichloromethane and aqueous methanol followed by (ii) size-exclusion chromatography. Fractions from chromatographic separation were screened for MTX toxicity using both the neuroblastoma neuro-2a (N2a) cytotoxicity and Ca2+ flux functional assays. Fractions containing MTX activity were analyzed using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) to pinpoint potential MTX analogs. Subsequent non-targeted HRMS analysis permitted the identification of a novel MTX analog, maitotoxin-4 (MTX4, accurate mono-isotopic mass of 3292.4860 Da, as free acid form) in the most toxic fractions. HRMS/MS spectra of MTX4 as well as of MTX are presented. In addition, crude methanolic extracts of five other strains of G. excentricus and 37 other strains representing one Fukuyoa species and ten species, one ribotype and one undetermined strain/species of Gambierdiscus were screened for the presence of MTXs using low resolution tandem mass spectrometry (LRMS/MS). This targeted analysis indicated the original maitotoxin (MTX) was only present in one strain (G. australes S080911_1). Putative maitotoxin-2 (p-MTX2) and maitotoxin-3 (p-MTX3) were identified in several other species, but confirmation was not possible because of the lack of reference material. Maitotoxin-4 was detected in all seven strains of G. excentricus examined, independently of their origin (Brazil, Canary Islands and Caribbean), and not detected in any other species. MTX4 may therefore serve as a biomarker for the highly toxic G. excentricus in the Atlantic area.

The main purpose of this macro-study is to shed light on the broad impact of books. For this purpose, the impact a very large collection of books (more than 200,000) has been analysed by using PlumX, an analytical tool providing a great number of different metrics provided by various tools. Furthermore, the study focuses on the evolution of the most significant measures and indicators over time. The results show usage counts in comparison to the other metrics are quantitatively predominant. Catalogue holdings and reviews represent a book’s most characteristic measures deriving from its increased level of impact in relation to prior results. Our results also corroborate the long half-life of books within the scope of all metrics, excluding views and social media. Despite of some disadvantages, PlumX has proved to be a very helpful and promising tool for assessing the broad impact of books, especially because of how easy it is to enter the ISBN directly as well as its algorithm to aggregate all the data generated by the different ISBN variations.

With the goal of observing the employment history of given individuals, a database was built from the longitudinal data files of the Survey on Living Conditions between 2007-2010 and 2008-2011. Only individuals with continuous presence in the data during the four years were included. Individuals under 16 and over 65 were excluded, as well as the population of the autonomous cities of Ceuta and Melilla. The longitudinal database includes 34,692 observations from 9,003 individuals in 17 regions during the financial crisis of 2007-2011 in Spain., This work was supported by the Health Department. Andalusian Board. 2013-2015 under Grant PI-0682-2012

Dataset from article "Contrasting effect of Saharan Dust and UVR on autotrophic picoplankton in Nearshore vs. Offshore waters of Mediterranean Sea ", Universidad de Granada. Departamento de Ecología. Grupo de Investigación: RNM367

Datos complementarios del artículo: “Acquisition of Uropygial Gland Microbiome by Hoopoe Nestlings” publicado en Microbial Ecology (ISSN:0095-3628). DOI: https://doi.org/10.1007/s00248-017-1125-5

All data are own production except for ieee118.raw, Transient Stability Constrained Optimal Power Flow (TSCOPF) is a nonlinear optimization problem used to perform economic dispatches while ensuring transient stability. This paper proposes a multi-contingency TSCOPF model that retains the dynamics of all generators and includes a transient synchronous generator dq-axis model. Program write_tscopf.py automatically reads the system data from standard PSSE raw files, builds the TSCOPF model tscopf.gms on high-level modelling system GAMS and solves it using a non-heuristic Interior Point algorithm. This approach facilitates the application of the model to a variety of systems and scenarios. Data for the New England 39 Bus System and the IEEE 118 Bus system are included.

Este documento se trata de la base de datos (en inglés) utilizada para la elaboración del manuscrito "Persistence of submerged macrophytes in a drying world: unravelling the timing and the environmental drivers for the production of drought-resistant propagules", elaborado por S. Calero, L.P.C. Morellato y M.A. Rodrigo, y que será próximamente publicado en la revista internacional Aquatic Conservation: Marine and Freshwater Ecosystems., This dataset contains two years of data about the sexual reproductive phenology of three charophyte species (Chara hispida, Chara aspera and Nitella hyalina) growing in different depth and salinity conditions in two Mediterranean brackish shallow ponds. The first sheet contains all metadata. From the second to the seventh sheets, raw data from each population are shown. From the eighth to the thirteenth sheets, grouped and transformed data from each population -ready to perform circular statistics- are shown. In the last sheet, environmental and phenological variables from one population are shown. More information about the acquisition, analyses and discussion of these data is available in the manuscript: Calero, S., Morellato, L.P.C. & Rodrigo, M.A. Persistence of submerged macrophytes in a drying world: unravelling the timing and the environmental drivers for the production of drought-resistant propagules. Aquatic Conservation: Marine and Freshwater Ecosystems., Esta base de datos contiene dos años de datos sobre la fenología reproductiva de tres especies de carófitos (Chara hispida, Chara aspera y Nitella hyalina) que crecen en distintas condiciones de profundidad y salinidad en dos charcas mediterráneas (someras y de agua salobre). La primera hoja de cálculo contiene todos los metadatos. De la segunda a la séptima hoja, se muestran los datos en bruto de cada población. De la octava a la decimotercera hoja, se muestran los datos agrupados y transformados de cada población. En la última hoja, se muestran las variables ambientales y fenológicas correspondientes a una población. Puede encontrarse más información sobre la adquisición, el análisis y la discusión de estos datos en el manuscrito: Calero, S., Morellato, L.P.C. & Rodrigo, M.A. Persistence of submerged macrophytes in a drying world: unravelling the timing and the environmental drivers for the production of drought-resistant propagules. Aquatic Conservation: Marine and Freshwater Ecosystems.

Arabidopsis_data_279accessions_20jan16, Genetic diversity provides insight into heterogeneous demographic and adaptive history across organisms’ distribution ranges. For this reason, decomposing single species into genetic units may represent a powerful tool to better understand biogeographical patterns as well as improve predictions of the effects of GCC (global climate change) on biodiversity loss. Using 279 georeferenced Iberian accessions, we used classes of three intraspecific genetic units of the annual plant Arabidopsis thaliana obtained from the genetic analyses of nuclear SNPs (single nucleotide polymorphisms), chloroplast SNPs, and the vernalization requirement for flowering. We used SDM (species distribution models), including climate, vegetation, and soil data, at the whole-species and genetic-unit levels. We compared model outputs for present environmental conditions and with a particularly severe GCC scenario. SDM accuracy was high for genetic units with smaller distribution ranges. Kernel density plots identified the environmental variables underpinning potential distribution ranges of genetic units. Combinations of environmental variables accounted for potential distribution ranges of genetic units, which shrank dramatically with GCC at almost all levels. Only two genetic clusters increased their potential distribution ranges with GCC. The application of SDM to intraspecific genetic units provides a detailed picture on the biogeographical patterns of distinct genetic groups based on different genetic criteria. Our approach also allowed us to pinpoint the genetic changes, in terms of genetic background and physiological requirements for flowering, that Iberian A. thaliana may experience with a GCC scenario applying SDM to intraspecific genetic units., Peer reviewed

AFLP profiles for the plants of Lavandula latifolia sampled for the study Markers are identified by primer combination and size (base pairs) AFLP.scores.xlsx MSAP profiles for the plants of Lavandula latifolia sampled for the study Markers are identified by primer combination and size (base pairs). MSAP.scores.xlsx Spatial coordinates of Lavandula latifolia plants sampled for the study Coordinates are expressed in meters and the origin (0,0) is the lower left corner of the plot as depicted in the figures. Plant.coordinates.xlsx, Little is known on the potential of ecological disturbance to cause genetic and epigenetic changes in plant populations. We take advantage of a long-term field experiment initiated in 1986 to study the demography of the shrub Lavandula latifolia, and compare genetic and epigenetic characteristics of plants in two adjacent subplots, one experimentally disturbed and one left undisturbed, 20 years after disturbance. Experimental setup was comparable to an unreplicated ‘Before-After-Control-Impact’ (BACI) design where a single pair of perturbed and control areas were compared. When sampled in 2005, plants in the two subplots had roughly similar ages, but they had established in contrasting environments: dense conspecific population (‘Undisturbed’ subpopulation) versus open area with all conspecifics removed (‘Disturbed’ subpopulation). Plants were characterized genetically and epigenetically using amplified fragment length polymorphism (AFLP) and two classes of methylation-sensitive AFLP (MSAP) markers. Subpopulations were similar in genetic diversity but differed in epigenetic diversity and multilocus genetic and epigenetic characteristics. Epigenetic divergence between subpopulations was statistically unrelated to genetic divergence. Bayesian clustering revealed an abrupt linear boundary between subpopulations closely coincident with the arbitrary demarcation line between subplots drawn 20 years back, which supports that genetic and epigenetic divergence between subpopulations was caused by artificial disturbance. There was significant fine-scale spatial structuring of MSAP markers in both subpopulations, which in the Undisturbed one was indistinguishable from that of AFLP markers. Genetic differences between subpopulations could be explained by divergent selection alone, while the concerted action of divergent selection and disturbance-driven appearance of new methylation variants in the Disturbed subpopulation is proposed to explain epigenetic differences. This study provides the first empirical evidence to date suggesting that relatively mild disturbances could leave genetic and epigenetic signatures on the next adult generation of long-lived plants., Peer reviewed