BUSCADOR RECOLECTA

Es una portada de revista, actividad de difusión., A multifunctional graphene conjugate was designed for cancer treatment, as reported by E. Miyako, A. Bianco, and co-workers in their Communication on page 14034. The image depicts the three regalia–crown, orb, and sword–representing triply chemically modified graphene as the strongest approach for cancer therapy., A multifunctional graphene conjugate was designed for cancer treatment, as reported by E. Miyako, A. Bianco, and co-workers in their Communication on page 14034. The image depicts the three regalia–crown, orb, and sword–representing triply chemically modified graphene as the strongest approach for cancer therapy.

En este trabajo se clonaron y caracterizaron tres lacasas bacterianas de las especies Pediococcus parvulus, Lacticaseibacillus paracasei y Lactococcus lactis, aisladas de vino y queso, y se evaluó su habilidad degradadora de aminas biógenas en presencia y ausencia de los mediadores ABTS (artificial) y epicatequina (natural)., In this study, we cloned and characterized three bacterial laccases from strains of the species Pediococcus parvulus, Lacticaseibacillus paracasei and Lactococcus lactis isolated from wine and cheese, and evaluated their biogenic amine degradation abilities in the presence/absence of artificial 2,2’-azinobis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) or natural (epicatechin) mediator compounds. Although some recombinant bacterial laccases have been characterized and re-vealed as biological tools to degrade biogenic amines using or not mediators, no previous research had been undertaken on the role of natural mediators, such as phenolic substrates found in wine and some vegetable food, in the degradation of biogenic amines. The three recombinant bacterial laccases exhibited sigmoidal kinetics, similar molecular mass, and different k0.5 and specific activi-ties on ABTS. They are acidophilic with optima temperature of 28ºC, and have low thermal stabil-ity at temperatures higher than 37ºC. The three laccases were able to degrade dopamine in ab-sence of any mediator, whereas the rest of amines were not degraded. The presence of ABTS somehow improved dopamine and tyramine degradation, while the addition of epicatechin did not improve their degradation. This is the first study in which the biogenic amine-degrading effi-ciency of an artificial mediator used by laccases is compared with a natural mediator.