BUSCADOR RECOLECTA

8 programes especials del programa "Fora d'hores" de Ràdio 4 produït pel "Laboratori de Produccions Sonores de Ficció i Entreteniment" de la Llicenciatura de Comunicació Audiovisual de la UAB., Emesos el curs 2011-2012.

Article to this study can be found at http://hdl.handle.net/10481/23726, Complementary material of a study which analyzes the BKCI according to the citation distribution of book chapters.

Material complementario del estudio "Análisis de las universidades españolas de acuerdo a su perfil de publicación en revistas por áreas científicas". Los indicadores bibliométricos utilizados se basan en las revistas indexadas en los JCR por una universidad dentro de un área en el periodo 2007-2011.

Experimental Procedures for Supplemental Figure: Double-stranded Zic2 and Zic2 mutant decoy DNA were prepared by annealing complementary single strands with the follow sequences: 5′-TCTTGGGTGGTCTCCGGAGACCACCCAAGA (for Zic2 decoy) and 5′-TCTTGAGTGAACTCCGGAGTTCACTCAAGA (for Zic2M decoy) as a negative control (Mizugishi et al., 2001). We utilized unlabeled and also Texas-red-tagged oligonucleotides (Invitrogen) to monitor DNA entry into cells. The terminal four bases on either site of the molecule were linked by phosphorothioate esters for added stability. Retinal explants were exposed to serial concentrations (0, 10, 100, and 200 μM) of decoy DNA combined with serial concentrations of serum-free medium (SFM) plus Fungene 6 transfection reagent (Roche) and incubated for 1 hr 30 min at 37°C. The explants were then washed several times in SFM, plated on D-polylysine/laminin, and incubated at 37°C, and dissociated chiasm cells were added to the cultures 2 hr later. They were then immunolabeled with α-neurofilament and explant outgrowth quantified, as indicated in Experimental Procedures., Figure S1. Zic2 Is Necessary for Ventrotemproal (VT) RGCs to Respond Negatively to Cues from the Chiasm Midline In Vitro. (A) Retinal explants grown on dissociated chiasm cells and immunostained with α-neurofilament. Representative images of dorsotemporal (DT) (a–d) and VT (e–h) retinal explants that were untransfected (a and e) or incubated with lipofectamine (b and f), Zic2 decoy oligos (c and g), or Zic2-mutant-decoy oligos (d and h) and subsequently cocultured with dissociated chiasm cells. Scale bar: 100 μm. (B) Quantification of area occupied by axons of DT (black columns) or VT (white columns) retinal explants that were untransfected or incubated with lipofectamine, Zic2-decoy oligos, or mutant-Zic2-decoy oligos, and then cocultured with dissociated chiasm cells. Left panels and graph: transfection of Zic2-decoy oligos does not affect the outgrowth in DT neurites (p > 0.2). Right panels and graph: transfection of Zic2-decoy oligos leads to increased growth of VT neurites. (*p < 0.05 compared with VT explants incubated with the lipofection agent alone or transfected with Zic2M-decoy). Number above bars indicates number of explants., Peer reviewed

Analytical and spectral characterization data of all new compounds, NMR/NOESY interprotonic distances, and crystallographic data (CIF)., Peer reviewed

Peer reviewed

Peer reviewed

Preparation procedures and full characterization data for compounds 3a–c, 4a–c, and 9a. NMR spectra of 3a–c. Cyclic voltammogram analysis data. Gaussian output data of structures 5–7., Peer reviewed

Figure S1 Multiple amino acid alignment of the P1 proteins of 97 isolates belonging to seven Plum pox virus strains recovered from public databases. The amino acid conservation at positions W29 and V139E is highlighted. Figure S2 Alignment of small RNAs to the reference PS MCL genome. The green bars represent the unique small RNAs (length between 20 and 25 bases) aligned to the two strands of the PS MCL genome. Only perfect matching was allowed., Peer reviewed

Supplementary methods. Table S1. Primers and templates used for PCRs. Table S2. Sequences of PCR primers used in the construction of chimerical viruses. Table S3. List of primers and templates used in PCRs to generate plasmids based on pMDC32. Table S4. Sequences of PCR primers used to construct plasmids based on pMDC32. Fig. S1. Infectivity of chimerical viruses based on plum pox virus. Fig. S2. Infectivity of chimerical viruses based on plum pox virus. Fig. S3. Anti-silencing activity of CaMV P6 proteins., Peer reviewed