Resultados totales (Incluyendo duplicados): 4
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Encontrada(s) 1 página(s)
CORA.Repositori de Dades de Recerca
doi:10.34810/data1055
Dataset. 2024
DATA ABOUT MAMMALIAN CELL INTERACTION PROMOTED BY A RECOMBINAN CALRETICULIN
- Parladé Molist, Eloi
- García-León, Annabel
- Voltà-Durán, Eric
- Unzueta Elorza, Ugutz
- Mangues, Ramon
- Casanova Rigat, Isolda
- Villaverde, Antonio
- Vazquez, Esther
Set of data investigating the cell interactivity of a recombinant calreticulin.
1. Description of methods used for collection-generation of data:
In vivo biodistribution: n this study, nanoparticle biodistribution was examined in an Acute Myeloid Leukemia mouse model. After injecting THP-1 cells, tumor growth was monitored, and when tumors reached 300-400 mm^3, mice received a single intravenous dose (buffer, 200 mg GFP-H6, or 200 mg V1-GFP-pcCRT-H6). Mice were euthanized at 1, 5, and 24 hours post-injection. Fluorescence intensity, indicating nanoparticle accumulation, was measured in tissues using the IVIS Spectrum 200 Imaging System. The results were determined by subtracting autofluorescence observed in control mice injected with buffer.
Confocal microscopy: In confocal microscopy, HeLa cells on MatTek plates were exposed to proteins of interest in OptiProTM medium for 2 to 24 hours. Cell nuclei were labeled with Hoechst 33342, and plasma membranes were stained with CellMask™ Deep Red. Imaging was done with a TCS SP5 Leica Spectral confocal microscope. Excitation used 405 nm (Hoechst), 488 nm (GFP), and 633 nm (CellMask™) lasers. Detection voltages were optimized to avoid cross-talk.
The rest of methods are standard and well described elsewhere: https://doi.org/10.1016/j.biopha.2023.114976
2. Methods for processing the data:
Data processing details are standard and well described elsewhere: https://doi.org/10.1016/j.biopha.2023.114976
3. Instrument- or software- specific information needed to interpret the data:
Leica Application Suite X, version 3.5.7, available at: https://www.leica-microsystems.com/es/productos/software-de-microscopia/p/leica-las-x-ls/downloads/
GraphPad Prism, version 8.0.2, available at: https://www.graphpad.com/
Cytexpert, version 2.4, available at: https://www.beckman.es/flow-cytometry/research-flow-cytometers/cytoflex/software?country=ES
4. Instruments, calibration and standards information:
EmulsiFlex-C5 system (Avestin)
ÄKTA pure system (Cytiva)
Zetasizer Pro (Malvern Analytical)
IVIS Spectrum 200 Imaging System (PerkinElmer)
TCS SP5 Leica Spectral confocal microscope (Leica Microsystems)
CytoFLEX Flow Cytometer (Beckman Coulter)
5. Environmental or experimental conditions:
Data processing and figure creation were performed in Windows 10 OS.
Proyecto: //
CORA.Repositori de Dades de Recerca
doi:10.34810/data1196
Dataset. 2024
REPLICATION DATA FOR "CITRATE-ASSISTED REGULATION OF ENDOCRINE-LIKE FUNCTIONS IN BIO-MIMETIC AMYLOIDS"
- de Pinho Favaro, Marianna T.
- López-Laguna, Hèctor
- Voltà-Durán, Eric
- Alba-Castellón, Lorena
- Sánchez, Julieta M.
- Casanova Rigat, Isolda
- Unzueta Elorza, Ugutz
- Mangues, Ramon
- Villaverde Corrales, Antonio
- Vázquez Gómez, Esther
Raw data of a set of experiments aimed to investigate the citrate-assisted regulation of endocrine-like functions in bio-mimetic amyloids.
Generic methodologies used here are similar to those described in:
- https://doi.org/10.1002/advs.201902420
- https://doi.org/10.1002/adfm.202100914
- https://doi.org/10.1080/10717544.2021.1998249
Proyecto: //
CORA.Repositori de Dades de Recerca
doi:10.34810/data585
Dataset. 2022
RAW DATA TO SUPPORT THE MANUSCRIPT WITH THE FOLLOWING INTENDED TITLE: HIGH-PRECISION TARGETING OF CANCER-ASSOCIATED PDGFR-Β+ STROMAL FIBROBLASTS THROUGH SELF-ASSEMBLING, PROTEIN-ONLY NANOPARTICLES
- Voltà-Durán, Eric
- Alba-Castellón, Lorena
- Serna, Naroa
- Casanova Rigat, Isolda
- López-Laguna, Hèctor
- Gallardo, Alberto
- Sánchez Chardi, Alejandro
- Villaverde Corrales, Antonio
- Unzueta Elorza, Ugutz
- Vázquez Gómez, Esther
- Mangues, Ramon
Raw data of a set of experiments aimed to target cancer-associated PDGFR-β+ stromal fibroblasts. Generic methodologies used here are similar to those described in doi: 10.2147/IJN.S34450
Proyecto: //
CORA.Repositori de Dades de Recerca
doi:10.34810/data735
Dataset. 2023
BIOLOGICAL EFFECTS OF CYTOTOXIC INFLAMMATORY PROTEINS FORMULATED AS SELF-ASSEMBLING, PROTEIN-ONLY NANOPARTICLES
- Martínez-Torró, Carlos
- Alba-Castellón, Lorena
- Carrasco-Díaz, Luis Miguel
- Serna, Naroa
- Imedio, Laura
- Gallardo, Alberto
- Casanova Rigat, Isolda
- Unzueta, Ugutz
- Vazquez, Esther
- Mangues, Ramon
- Villaverde, Antonio
Data describing the biological effects of cytotoxic inflammatory proteins formulated as self-assembling, protein-only nanoparticles
Proyecto: //
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