BUSCADOR RECOLECTA

Fig2A_cpDNA_Platycodoneae NEXUS and tre files with the analysis settings used in MrBayes inferred from the concatenated chloroplast dataset (psbJ-petA, trnL-trnF, petB-petD) for the Platycodoneae Fig2B_ITS_platycodoneae NEXUS and tre files with the analysis settings used in MrBayes inferred from the nuclear ribosomal dataset (ITS) for Platycodoneae Fig2C_ITS_4cpdna NEXUS and tre files with the analysis settings used in MrBayes inferred from the combined nuclear and chloroplast dataset (ITS, psbJ-petA, trnL-trnF, petB-petD, trnS-trnG) for Platycodoneae Nested-dating approach analysis Script (.xml) and tre files for the "Nested analyses" of all three linked datasets: Platycodoneae, C. eminii and C. canariensis Nested Analysis.zip Figure S1 Nexus and .tre files for the single-gene analyses of the Platycodoneae dataset. Figure S2 Nexus and .tre files for the single-gene analyses of the Canarina dataset, Transoceanic distributions have attracted the interest of scientists for centuries. Less attention has been paid to the evolutionary origins of ‘continent-wide’ disjunctions, in which related taxa are distributed across isolated regions within the same continent. A prime example is the ‘Rand Flora’ pattern, which shows sister taxa disjunctly distributed in the continental margins of Africa. Here, we explore the evolutionary origins of this pattern using the genus Canarina, with three species: C. canariensis, associated with the Canarian laurisilva, and C. eminii and C. abyssinica, endemic to the Afromontane region in East Africa, as case study. We infer phylogenetic relationships, divergence times and the history of migration events within Canarina using Bayesian inference on a large sample of chloroplast and nuclear sequences. Ecological niche modelling was employed to infer the climatic niche of Canarina through time. Dating was performed with a novel nested approach to solve the problem of using deep time calibration points within a molecular dataset comprising both above-species and population-level sampling. Results show C. abyssinica as sister to a clade formed by disjunct C. eminii and C. canariensis. Miocene divergences were inferred among species, whereas infraspecific divergences fell within the Pleistocene–Holocene periods. Although C. eminii and C. canariensis showed a strong genetic geographic structure, among-population divergences were older in the former than in the latter. Our results suggest that Canarina originated in East Africa and later migrated across North Africa, with vicariance and aridification-driven extinction explaining the 7000 km/7 million year divergence between the Canarian and East African endemics., Peer reviewed

Matrix AFLPs Canarina canariensis Original Matrix of Canarina canariensis AFLPs matriz_595alelos.xlsx cpDNA_Canarina_canariensis NEXUS files for the concatenated cpDNA dataset of Canarina canariensis, Geographical isolation by oceanic barriers and climatic stability has been postulated as some of the main factors driving diversification within volcanic archipelagos. However, few studies have focused on the effect that catastrophic volcanic events have had on patterns of within-island differentiation in geological time. This study employed data from the chloroplast (cpDNA haplotypes) and the nuclear (AFLPs) genomes to examine the patterns of genetic variation in Canarina canariensis, an iconic plant species associated with the endemic laurel forest of the Canary Islands. We found a strong geographical population structure, with a first divergence around 0.8 Ma that has Tenerife as its central axis and divides Canarian populations into eastern and western clades. Genetic diversity was greatest in the geologically stable ‘palaeo-islands’ of Anaga, Teno and Roque del Conde; these areas were also inferred as the ancestral location of migrant alleles towards other disturbed areas within Tenerife or the nearby islands using a Bayesian approach to phylogeographical clustering. Oceanic barriers, in contrast, appear to have played a lesser role in structuring genetic variation, with intra-island levels of genetic diversity larger than those between-islands. We argue that volcanic eruptions and landslides after the merging of the palaeo-islands 3.5 Ma played key roles in generating genetic boundaries within Tenerife, with the palaeo-islands acting as refugia against extinction, and as cradles and sources of genetic diversity to other areas within the archipelago., Peer reviewed

Photographs taken with a digital camera Nikon Coolpix 950 adapted to a binocular Nikon SMZ. A list attached includes exact locations where seeds were gathered. This dataset is under a Creative Commons Attribution-Non commercial 4.0 International License. 304 jpg files included., Analysis of seed shape in two subspecies of Capparis spinosa collected in Tunisia: Five populations of Subspecies Spinosa; five populations of Subspecies Rupestris. Morphological description of seeds is a required step for the analysis of biodiversity in natural populations and may give clues to understand adaptive strategies in evolution. The cardioid is the curve described by a point of one circumference rolling around another circumference of equal radius. Models based in the adjustment of seed shape with cardioid curves where described previously for Arabidopsis thaliana and the model legumes Lotus japonicus and Medicago truncatula. In this work the model is applied to the morphology of seeds in populations from two subspecies of Capparis spinosa grown in Tunisia. The adjustment of seed images to cardioid curves, followed by statistical analysis of similarity in the complete images as well as in each of four quadrants, allows an accurate description of seed shape. Results show differences in morphology between subspecies. Seeds from Capparis spinosa. subsp. rupestris present higher diversity in shape than seeds from populations of C. subsp. spinosa. This may indicate a primitive condition in the seeds of C. subsp. rupestris associated with non-specialization. The results are discussed in relation to ecological strategies of both subspecies in evolution., Funded through a cooperation between IRNASA-CSIC and National Institute of Research in Rural Engineering, Waters and Forests (INRGREF), University of Carthage, Tunisia., Peer reviewed

Photographs taken with a digital camera Nikon Coolpix 950. This dataset is under a Creative Commons Attribution-Non commercial 4.0 International License.51 jpg files included, Eight accessions of Jatropha curcas (six American and two African) were sown in the south of Tunisia (Gabès) to study its agricultural properties and development under local semi-arid conditions. Traits observed included general aspects of plant development (plant height, canopy circumference, number of nodes and seed yield); leaf characteristics (petiole length, limb length, limb width and foliar surface); and seed traits (size and shape). Morphological analysis of seeds included seed image area, perimeter, length/width ratio, circularity index and J index. This index reflects the similarity of seed images with an ellipse showing differences in seed shape between accessions. Jatropha curcas genotypes introduced in Tunisia are polymorphic, their seed yield is low and variable between genotypes. Seed morphology analysis gave an indication in relation with the productivity in some of the accessions. The comparative agronomic studies are crucial before early selection., Funded through a cooperation between IRNASA-CSIC and National Institute of Research in Rural Engineering, Waters and Forests (INRGREF), University of Carthage, Tunisia., Sí