BUSCADOR RECOLECTA

Experimental Procedures for Supplemental Figure: Double-stranded Zic2 and Zic2 mutant decoy DNA were prepared by annealing complementary single strands with the follow sequences: 5′-TCTTGGGTGGTCTCCGGAGACCACCCAAGA (for Zic2 decoy) and 5′-TCTTGAGTGAACTCCGGAGTTCACTCAAGA (for Zic2M decoy) as a negative control (Mizugishi et al., 2001). We utilized unlabeled and also Texas-red-tagged oligonucleotides (Invitrogen) to monitor DNA entry into cells. The terminal four bases on either site of the molecule were linked by phosphorothioate esters for added stability. Retinal explants were exposed to serial concentrations (0, 10, 100, and 200 μM) of decoy DNA combined with serial concentrations of serum-free medium (SFM) plus Fungene 6 transfection reagent (Roche) and incubated for 1 hr 30 min at 37°C. The explants were then washed several times in SFM, plated on D-polylysine/laminin, and incubated at 37°C, and dissociated chiasm cells were added to the cultures 2 hr later. They were then immunolabeled with α-neurofilament and explant outgrowth quantified, as indicated in Experimental Procedures., Figure S1. Zic2 Is Necessary for Ventrotemproal (VT) RGCs to Respond Negatively to Cues from the Chiasm Midline In Vitro. (A) Retinal explants grown on dissociated chiasm cells and immunostained with α-neurofilament. Representative images of dorsotemporal (DT) (a–d) and VT (e–h) retinal explants that were untransfected (a and e) or incubated with lipofectamine (b and f), Zic2 decoy oligos (c and g), or Zic2-mutant-decoy oligos (d and h) and subsequently cocultured with dissociated chiasm cells. Scale bar: 100 μm. (B) Quantification of area occupied by axons of DT (black columns) or VT (white columns) retinal explants that were untransfected or incubated with lipofectamine, Zic2-decoy oligos, or mutant-Zic2-decoy oligos, and then cocultured with dissociated chiasm cells. Left panels and graph: transfection of Zic2-decoy oligos does not affect the outgrowth in DT neurites (p > 0.2). Right panels and graph: transfection of Zic2-decoy oligos leads to increased growth of VT neurites. (*p < 0.05 compared with VT explants incubated with the lipofection agent alone or transfected with Zic2M-decoy). Number above bars indicates number of explants., Peer reviewed

Analytical and spectral characterization data of all new compounds, NMR/NOESY interprotonic distances, and crystallographic data (CIF)., Peer reviewed

[CAT] Dades antracològiques procedents de l'excavació d'urgència de Can Viola, realitzat l'any 2003 en el context de l'obra d'ampliació de la variant C-59. Excavació d'urgència dirigida per Arcos, R. i Velasco, C. Les dades adjuntades provenen del registre antracològic trobat a l'interior d'un forn datat al Bronce Final (900-650 aC) en la unitat estratigrafia UE-184.

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, [ENG] Anthracological data from the rescue excavation at Can Viola, carried out in 2003 in the context of the expansion works of the C-59 highway. Emergency excavation directed by Arcos, R. and Velasco, C. The attached data come from the anthracological record found inside a furnace dated to the Late Bronze Age (900-650 BC) in the UE-184 stratigraphic unit.

Anthracological data from the rescue excavations at Catillo de Olmos (Toledo). The attached data come from the anthracological and carpological records from an islamic fortress.

Dades d'ús de prèstec del CRAI de la UB de l'any 2002. Inclou en format csv les dades mensuals i el recull anual. Dades desglossades per: tipus d'operació, tipus de lector, per tipus de material, llengua i any de publicació. Les dades corresponents al mes de maig no estan disponibles

Dades relatives al nombre de cerques al catàleg de fons modern del CRAI de la UB durant el curs 2002-2003. Inclou en format csv les dades mensuals desglossades per tipus de cerca i per la ubicació des d'on s'ha fet la consulta.

The wide-angle seismic reflection data set consists of two transects. Transect A coincides with the trace of the IBERSEIS deep seismic reflection profile and transect B is located farther to the SE. The explosive charge was located in a single 20 cm diameter, 50–60-m deep shothole. Explosive shots with charge sizes of 1000, 750 and 500 kg, where distributed along the transect, with the largest charges located at the edges of the profile. 650 digital seismic recorders (590 Texans and 60 3-component Refteks) from IRIS (Incorporated Research Institutions for Seismology) instrument pool where used. These instruments where placed along the 300-km transect A at a 400-m station spacing. For transect B, a denser trace spacing was used. To achieve this, shots were fired twice. The stations were firstly placed at 300–400 m spacing along transect B. Once the shots were fired, the stations were moved 150–200 m toward the north along the profile. All shots were then fired a second time, resulting in shot records with a 150–200 m trace spacing., The IBERSEIS wide-angle seismic reflection dataset consists of two NE-SW oriented transects acquired in the SW of the Iberian Massif. They crossed three major geological zones (South Portuguese Zone, Ossa-Morena Zone, and Central Iberian Zone), with their tectonic contacts and the Pyrite Belt being of greatest interest. The velocity models obtained by forward modeling show a complex crust, especially in the middle crust. The velocity models are the most detailed ones that have been produced in the area and contain a large amount of new features that are relevant to the understanding of the composition of the crust and upper mantle beneath the zone., Spanish Ministry of Science and Innovation, CGL2004-04623, CSD2006-00041.-- Generalitat de Catalunya.-- Junta de Andalucía.--, Peer reviewed

RECS-1 Cruise (29GD20030329) carried out on the Research Vessel García del Cid in 2003, Characterize the temporal and spatial variability of water and particle flows in the area of the Blanes canyon. Determine the seasonal and suvestational variability of the population structure of the shrimp Aristeus antennatus. Interaction of the submarine canyon environment with the physiological state of the associated shrimp resource. Spatio-temporal determination of the population structure of shrimp linked to the submarine canyon. Detection of recruitment areas and application of new technologies for their preservation

SAVOR-1 Cruise (29GD20030602) carried out on the Research Vessel García del Cid in 2003, Study of the reproduction of anchovy

SAVOR-2 Cruise (29GD20030616) carried out on the Research Vessel García del Cid in 2003, Study of the reproduction of anchovy