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This Excel file includes the data and tracked calculations of the manuscript entitled "Sponge contribution to the silicon cycle of a diatom-rich shallow bay". It includes 7 spreadsheets with the following contents: - READ ME - Standing STOCK living sponges - Sponge Si consumption FLUX - Si RESERVOIR in sediments - Sponge Si FLUXES in sediments - DIATOM Si fluxes&stocks (Fig.5) - Calculations for discussion, This research was supported by: - the Spanish Ministry grants CTM2015-67221-R and MICIU: #PID2019-108627RB-I00 to Manuel Maldonado - the grant 12735 – AO2020 of the French National research program EC2CO to Jacques Grall - the ISblue project, Interdisciplinary graduate school for the blue planet (ANR-17-EURE-0015), co-funded by a grant from the French government under the program "Investissements d'Avenir", and the “Xunta de Galicia” postdoctoral grant IN606B-2019/002 to María López-Acosta., Peer reviewed

This Excel file includes the metadata of the survey of the predation activity of the nudibranch Doris verrucosa on the sponge Hymeniacidon perlevis, This research was supported by: - the grant 12735 – AO2020 of the French National research program EC2CO - the ISblue project, Interdisciplinary graduate school for the blue planet (ANR-17-EURE-0015), co-funded by a grant from the French government under the program "Investissements d'Avenir", and the “Xunta de Galicia” postdoctoral grant IN606B-2019/002, No

[Description of methods used for collection/generation of data] In both stations a SAMI-pH (Sunburst Sensors LCC) was attached, at 1 m in the Bay of Palma and at 4 m depth in Cabrera. The pH sensors were measuring pH, in the total scale (pH𝑇T), hourly since December 2018 in the Bay of Palma and since November 2019 in Cabrera. The sensor precision and accuracy are < 0.001 pH and ± 0.003 pH units, respectively. Monthly maintenance of the sensors was performed including data download and surface cleaning. Temperature and salinity for the Cabrera mooring line was obtained starting November 2019 with a CT SBE37 (Sea-Bird Scientific©). Accuracy of the CT is ± 0.002 ∘C for temperature and ± 0.003 mS cm−1−1 for conductivity. Additionally, oxygen data from a SBE 63 (Sea-Bird Scientific ©) sensor attached to the CT in Cabrera were used. Accuracy of oxygen sensors is ± 2% for the SBE 63., [Methods for processing the data] Periodically water samplings for dissolved oxygen (DO), pH in total scale at 25 ∘C (pH𝑇25) and total alkalinity (TA) were obtained during the sensor maintenance campaigns. DO and (pH𝑇25) samples were collected in order to validate the data obtained by the sensors. DO concentrations were evaluated with the Winkler method modified by Benson and Krause by potentiometric titration with a Metrohm 808 Titrando with a accuracy of the method of ± 2.9 μmol kg−1μmol kg−1 and with an obtained standard deviation from the sensors data and the water samples collected of ± 5.9 μmol kg−1μmol kg−1. pH𝑇25T25 data was obtained by the spectrophotometric method with a Shimadzu UV-2501 spectrophotometer containing a 25 ∘C-thermostated cells with unpurified m-cresol purple as indicator following the methodology established by Clayton and Byrne by using Certified Reference Material (CRM Batch #176 supplied by Prof. Andrew Dickson, Scripps Institution of Oceanography, La Jolla, CA, USA). The accuracy obtained from the CRM Batch was of ± 0.0051 pH units and the precision of the method of ± 0.0034 pH units. The mean difference between the SAMI-pH and discrete samples was of 0.0017 pH units., Funding for this work was provided by the projects RTI2018-095441-B-C21 (SuMaEco) and, the BBVA Foundation project Posi-COIN and the Balearic Islands Government projects AAEE111/2017 and SEPPO (2018). SF was supported by a “Margalida Comas” postdoctoral scholarship, also from the Balearic Islands Government. FFP was supported by the BOCATS2 (PID2019-104279GB-C21) project funded by MCIN/AEI/10.13039/501100011033.This work is a contribution to CSIC’s Thematic Interdisciplinary Platform PTI WATER:iOS., Peer reviewed

[Description of methods used for collection/generation of data] Data was acquired in both stations using a SAMI-pH (Sunburst Sensors LCC) was attached, at 1 m in the Bay of Palma and at 4 m depth in Cabrera. The pH sensors were measuring pH, in the total scale (pH𝑇), hourly since December 2018 in the Bay of Palma and since November 2019 in Cabrera. The sensor precision and accuracy are < 0.001 pH and ± 0.003 pH units, respectively. Monthly maintenance of the sensors was performed including data download and surface cleaning. Temperature and salinity for the Cabrera mooring line was obtained starting November 2019 with a CT SBE37 (Sea-Bird Scientific©). Accuracy of the CT is ± 0.002 ∘C for temperature and ± 0.003 mS cm−1−1 for conductivity. Additionally, oxygen data from a SBE 63 (Sea-Bird Scientific ©) sensor attached to the CT in Cabrera were used. Accuracy of oxygen sensors is ± 2% for the SBE 63., [Methods for processing the data] Once data (available at https://doi.org/XXX/DigitalCSIC/XXX) was validated, several processing steps were performed to ensure an optimal training process for the neural network models. First, all the data of the time series were re-sampled by averaging the data points obtaining a daily frequency. Afterwards, a standard feature-scaling procedure (min-max normalization) was applied to every feature (temperature, salinity and oxygen) and to pHT. Finally, we built our training and validations sets as tensors with dimensions (batchsize, windowsize, 𝑁features), where batchsize is the number of examples to train per iteration, windowsize is the number of past and future points considered and 𝑁features is the number of features used to predict the target series. Temperature values below 𝑇=12.5T=12.5 °C were discarded as they are considered outliers in sensor data outside the normal range in the study area. A BiDireccional Long Short-Term Memory (BD-LSTM) neural network was selected as the best architecture to reconstruct the pHT time series, with no signs of overfitting and achieving less than 1% error in both training and validation sets. Data corresponding to the Bay of Palma were used in the selection of the best neural network architecture. The code and data used to determine the best neural network architecture can be found in a GitHub repository mentioned in the context information., Funding for this work was provided by the projects RTI2018-095441-B-C21, RTI2018-095441-B-C22 (SuMaEco) and Grant MDM-2017-0711 (María de Maeztu Excellence Unit) funded by MCIN/AEI/10.13039/501100011033 and by the “ERDF A way of making Europe", the BBVA Foundation project Posi-COIN and the Balearic Islands Government projects AAEE111/2017 and SEPPO (2018). SF was supported by a “Margalida Comas” postdoctoral scholarship, also from the Balearic Islands Government. FFP was supported by the BOCATS2 (PID2019-104279GB-C21) project funded by MCIN/AEI/10.13039/501100011033.This work is a contribution to CSIC’s Thematic Interdisciplinary Platform PTI WATER:iOS (https://pti-waterios.csic.es/)., Peer reviewed

Tabla de datos, Peer reviewed

1 table., S2 Table. NAAIRS mutants of SAUR63 and root tortuosity index., Peer reviewed

1 table., S3 Table. Primers used., Peer reviewed

1 table., S4 Table. Clones for transformation., Peer reviewed

1 table., S5 Table. Antibodies., Peer reviewed

A) Genomic map showing positions of genes and locations of mutations from CRISPR/Cas9 mutagenesis in the 9x-saur mutant based on the TAIR10 Arabidopsis genome annotation. The first sgRNA directed cuts in both SAUR61 and SAUR64 (green arrows), creating a deletion between them (green bar) and leaving behind a hybrid gene with a frameshift at the junction site (symbolized by a green X). The second sgRNA directed cuts in the remaining genes (blue arrows, with lighter blue indicating slight mismatches between the sgRNA and the genome), leading to deletions (blue bars) and/or frameshift mutations (blue X’s). SAUR gene names are abbreviated as S61 etc. SAUR61-SAUR68 are on chromosome 1 and SAUR75 is on chromosome 5. B,C) 5-day-old seedlings grown on 1x MS/1% Suc medium in long days. Scale bar, 1 mm. D) Hypocotyl lengths of seedlings grown for 4d in short days on 0.5x MS medium. n, 27 (wild type), 22 (9x-saur). E) Cotyledon area of seedlings grown on vertically oriented plates for 6d on MS/1% Suc medium. n, 16 (wild type), 22 (9x-saur). Graphs show means ± s.d. No statistical differences were detected between wild-type and 9x-saur mutant measurements by t-test. F) Sequences of guide RNAs used for CRISPR/Cas9-mediated mutagenesis, wild-type genes, and mutant alleles present in the 9x-saur mutant. Underlines indicate PAM motif adjacent to guide RNA target site, and any mismatches to the guide RNA sequence. Uppercase bold letters indicate insertion mutations. All alleles create frameshift mutations except for saur75-1, which has an in-frame deletion of 13 amino acids in the SAUR domain., Peer reviewed