BUSCADOR RECOLECTA

Peer reviewed

Data showed are changes in the maturation index of CD83, CD86 and PD-L1 in allergic patient and tolerant control., Peer reviewed

Production of cytokine into the culture supernatant. In the case of TLR4, only significant increases of IL-5 were observed in allergic patients compared to tolerant controls when stimulating with TLR4lig-Pp3 and TLR4lig plus Pp3 peptide in separately way. No differences in the induction of IL-10 production were observed in allergic patients compared to tolerant controls, Peer reviewed

Production of cytokine into the culture supernatant from DCs. Our analysis in allergic patients compared to tolerant controls detected significant increases of IL-5 and IFN with all the conditions containing TLR7lig (TLR7lig, TLR7lig-Pp3 and TLR7lig plus Pp3 in separately way). we observed that TLR7lig-Pp3 induced significant lower IL-4 and IL-5 levels whilst higher IFNγ levels compared to the Pp3 only in allergic patients, indicating the specific immunomodulatory capacity of this compound., Peer reviewed

Data indicated that only TLR7lig-Pp3 led to significantly increased in the proliferation index of CD3+T-cells in allergic patients compared to tolerant controls, while TLR4lig-Pp3 seemed stimulate the T-cells proliferation without inducing a difference between groups. Moreover, in allergic patients there was a significant increase in CD3+ and CD4+ T-cells proliferation after stimulation with TLR4lig-Pp3 vs TLRlig and vs TLR4lig plus Pp3 peptide; additionally, TLR7lig-Pp3 induced a specific proliferative response of CD3+T-cells compared to TLR7lig and TLR7lig plus Pp3 peptide., Peer reviewed

Data indicated significantly higher percentages of Treg cells and IL-10 production in allergic patient compared to tolerant controls when stimulated with both TLRnlig-Pp3. Moreover, these effects were higher compared to Pp3 peptide alone, TLRnlig as well as TLRnlig plus Pp3 only in allergic patients except with TLR4lig-Pp3 for the IL-10 production. Regarding the TLR4 activation, they can induce a low production of IL-10., Peer reviewed

Data indicated an opposite result, since the combination of both TLRnlig-Pp3 (TLR7lig-Pp3 plus TLR4lig-Pp3) together decreased the maturation already produced by each compound alone, being significant for CD80, Peer reviewed

Regarding the effect of these interactions with DC on the proliferative response of the T-, B- and NK-cells, there was a decrease in comparison with the proliferation induced by the ligands incubated separately, Peer reviewed

There was a increase of IL-4 production and an decrease in the IFNγ level, Peer reviewed

Data observed a decrease of Treg percentages as well as the IL-10 production in response to the combination of TLR7lig-Pp3 plus TLR4lig-Pp3 compared to each TLRnlig-Pp3 in allergic patients, Peer reviewed