Resultados de investigación

[EN]A self-contained R Markdown document that reproduces the quantitative synthesis of a systematic review of quantitative anthrax (Bacillus anthracis) research in Africa. It performs random-effects (DerSimonian-Laird) meta-analyses of ecological niche / species distribution model discrimination (AUC, pooled on the logit scale and back-transformed) and of adjusted odds ratios for human behavioural risk factors (pooled on the log scale), with heterogeneity (Cochran's Q, I^2, tau^2, prediction intervals), pre-specified subgroup analyses, meta-regression, sensitivity analyses and publication-bias assessment (funnel plots, Egger's test, trim-and-fill), and generates all associated figures and tables. The 33 study-level records and the behavioural odds ratios are embedded in the document, so the analysis runs from the deposited files alone. Developed for, and illustrated with, a systematic review and meta-analysis of quantitative anthrax research in Africa, and reusable as a template for other meta-analyses of model performance and observational effect sizes., Meta_Analysis_Anthrax.Rmd; references.bib; vancouver-brackets.csl; Meta_Analysis_Anthrax.pdf; Supplementary_figures_geography_temporal.R; Supplementary_figures_network_covariate.R; MMDN_Screening.json; Reviewer_Screening.json; MMDN_RoB.json; Experts_RoB.json; id_58.json; id_63.json; README.txt; LICENSE.txt; CITATION.cff

[ES]Los datos son las respuestas de 182 profesores de Enseñanza Secundaria de Lengua y literatura españolas a un cuestionario sobre la enseñanza de la gramática y el discurso en sus aulas. Recoge unos metadatos (edad, género, años de ejercicio, formación universitaria, comunidad autónoma de trabajo) y 23 preguntas de carácter cuantitativo y cualitativo en relación con el peso de la enseñanza de la gramática en sus clases de lengua española y el interés y repercusión que tiene esta didáctica en las producciones escritas de los estudiantes., Esta investigación forma parte del proyecto GRAMADÍS, con referencia PID2023-149351NB-I00, financiado por el Ministerio de Ciencia, Innovación y Universidades, la Agencia Estatal de Investigación y el Fondo Europeo de Desarrollo Regional (MCIU /AEI /10.13039/501100011033 / FEDER, UE) dentro del Plan Estatal de Investigación Científica, Técnica y de Innovación 2021-2023, Este cuestionario está dirigido a profesores de Lengua y Literatura Castellana de Educación Secundaria Obligatoria. Se compone de 23 preguntas sobre la enseñanza de la gramática y el discurso en Educación Secundaria Obligatoria. Forma parte del proyecto de investigación de financiación estatal GRAMADÍS (PID 2023-149351NB-100).

Samples were acquired on a Cytek Aurora 5L spectral flow cytometer (Cytek Biosciences, Fremont, CA) equipped with five lasers (355 nm, 405 nm, 488 nm, 561 nm, 640 nm). Spectral unmixing was performed using SpectroFlo software (v3.3.0; Cytek). Flow cytometric data were analyzed using Infinicyt software version 2.1.0.a (BD Biosciences, San Jose, CA, USA). Raw data. Daily instrument quality control was performed using SpectroFlo QC beads (Cytek) before sample measurement. Single-stained reference controls and unstained controls were used for spectral unmixing validation., [EN]This dataset contains raw spectral flow cytometry files (FCS format) generated in a mouse immunization and challenge study evaluating an mRNA–solid lipid nanoparticle (mRNA-SLN) vaccine candidate encoding three MHC class II T-cell epitopes from Fasciola hepatica fused to eGFP (eGFP-Fh3Tq). BALB/c mice (n = 33) were allocated into four experimental groups: Untreated (n = 9), Infection control (n = 6), eGFP (n = 9), and eGFP-Fh3Tq (n = 9). Groups eGFP and eGFP-Fh3Tq received a prime–boost immunization regimen (21 days apart) with the corresponding mRNA-SLN formulation. Peripheral blood was collected from three groups (Untreated, eGFP, and eGFP-Fh3Tq) at day 1 post-vaccination (innate panel) and day 42 post-vaccination (adaptive panel); the Infection control group was not differentiated from the Untreated group at these time points and was therefore not sampled separately. For blood panels, samples from two mice were pooled and barcoded with anti-CD45 conjugated to either PerCP or BUV496 before acquisition, yielding three FCS files per group (6 mice/group). At day 42, spleens from three mice per group (Untreated, eGFP, eGFP-Fh3Tq) were harvested for intracellular cytokine staining (ICS) after ex vivo restimulation with the synthetic peptides T14, T15, and T16, PMA/ionomycin (positive control), or left unstimulated (negative control). For ICS, individual splenocyte samples from different conditions were barcoded with anti-CD45 and combined in pairs within each acquisition tube. Where sufficient cells were available, stimulation conditions were performed in duplicate. Remaining mice were orally challenged with 7 F. hepatica metacercariae for survival and protection assessment. All samples were acquired on a Cytek Aurora 5L spectral flow cytometer equipped with five lasers (355, 405, 488, 561, 640 nm)., Ministerio de Ciencia e Innovación Junta de Castilla y León, co-financed by the Fondo Social Europeo Plus Department of Education of the Basque Government, Contiene: Group key: G1 = Untreated (PBS control), G2 = eGFP (eGFP mRNA-SLN), G3 = eGFP-Fh3Tq (eGFP-Fh3Tq mRNA-SLN). R = biological replicate (individual mouse). Blood files contain two barcoded mice per tube (e.g., R1_R2). ICS files contain two barcoded conditions per tube. Stimulation conditions (ICS): PHAIono = PMA/ionomycin positive control; CTRL = unstimulated negative control; Pep = T14+T15+T16 peptide pool. Suffixes _1 and _2 denote technical duplicates of the same condition. Folder D1 — Peripheral blood, day 1 post-vaccination (innate panel) 1. PROT_Blood_D1_G1-Untreated_R1_R2_Unmixed.fcs 2. PROT_Blood_D1_G1-Untreated_R3_R4_Unmixed.fcs 3. PROT_Blood_D1_G1-Untreated_R5_R6_Unmixed.fcs 4. PROT_Blood_D1_G2-eGFP_R1_R2_Unmixed.fcs 5. PROT_Blood_D1_G2-eGFP_R3_R4_Unmixed.fcs 6. PROT_Blood_D1_G2-eGFP_R5_R6_Unmixed.fcs 7. PROT_Blood_D1_G3-eGFP-Fh3Tq_R1_R2_Unmixed.fcs 8. PROT_Blood_D1_G3-eGFP-Fh3Tq_R3_R4_Unmixed.fcs 9. PROT_Blood_D1_G3-eGFP-Fh3Tq_R5_R6_Unmixed.fcs Folder D42 — Peripheral blood, day 42 post-vaccination (adaptive panel) 1. PROT_Blood_D42_G1-Untreated_R1_R2_Unmixed.fcs 2. PROT_Blood_D42_G1-Untreated_R3_R4_Unmixed.fcs 3. PROT_Blood_D42_G1-Untreated_R5_R6_Unmixed.fcs 4. PROT_Blood_D42_G2-eGFP_R1_R2_Unmixed.fcs 5. PROT_Blood_D42_G2-eGFP_R3_R4_Unmixed.fcs 6. PROT_Blood_D42_G2-eGFP_R5_R6_Unmixed.fcs 7. PROT_Blood_D42_G3-eGFP-Fh3Tq_R1_R2_Unmixed.fcs 8. PROT_Blood_D42_G3-eGFP-Fh3Tq_R3_R4_Unmixed.fcs 9. PROT_Blood_D42_G3-eGFP-Fh3Tq_R5_R6_Unmixed.fcs Folder ICS — Splenocytes, day 42 post-vaccination (intracellular cytokine staining panel) 1. PROT_Spleen_ICS_01_G1R1-PHAIono1_G1R1-CTRL1_Unmixed.fcs 2. PROT_Spleen_ICS_02_G1R1-Pep1_G1R1-Pep2_Unmixed.fcs 3. PROT_Spleen_ICS_03_G1R2-PHAIono1_G1R2-PHAIono2_Unmixed.fcs 4. PROT_Spleen_ICS_04_G1R2-CTRL1_G3R1-CTRL1_Unmixed.fcs 5. PROT_Spleen_ICS_05_G1R2-Pep1_G1R2-Pep2_Unmixed.fcs 6. PROT_Spleen_ICS_06_G1R3-PHAIono1_G1R3-PHAIono2_Unmixed.fcs 7. PROT_Spleen_ICS_07_G1R3-CTRL1_G1R3-CTRL2_Unmixed.fcs 8. PROT_Spleen_ICS_08_G1R3-Pep1_G1R3-Pep2_Unmixed.fcs 9. PROT_Spleen_ICS_09_G2R1-PHAIono1_G2R1-CTRL1_Unmixed.fcs 10. PROT_Spleen_ICS_10_G2R1-Pep1_G2R1-Pep2_Unmixed.fcs 11. PROT_Spleen_ICS_11_G2R2-PHAIono1_G2R2-PHAIono2_Unmixed.fcs 12. PROT_Spleen_ICS_12_G2R2-CTRL1_G2R2-CTRL2_Unmixed.fcs 13. PROT_Spleen_ICS_13_G2R2-Pep1_G2R2-Pep2_Unmixed.fcs 14. PROT_Spleen_ICS_14_G2R3-PHAIono1_G2R3-PHAIono2_Unmixed.fcs 15. PROT_Spleen_ICS_15_G2R3-CTRL1_G2R3-CTRL2_Unmixed.fcs 16. PROT_Spleen_ICS_16_G2R3-Pep1_G2R3-Pep2_Unmixed.fcs 17. PROT_Spleen_ICS_17_G3R1-PHAIono1_G3R1-PHAIono2_Unmixed.fcs 18. PROT_Spleen_ICS_18_G3R1-Pep1_G3R1-Pep2_Unmixed.fcs 19. PROT_Spleen_ICS_19_G3R2-PHAIono1_G3R2-Pep1_Unmixed.fcs 20. PROT_Spleen_ICS_20_G3R2-CTRL1_G3R3-CTRL1_Unmixed.fcs 21. PROT_Spleen_ICS_21_G3R3-PHAIono1_G3R3-PHAIono2_Unmixed.fcs 22. PROT_Spleen_ICS_22_G3R3-Pep1_G3R3-Pep2_Unmixed.fcs

[EN]The dataset contents the records retrieved and selected after bibliographical queries about territorial governance of sustainable food networks. Query: ("ALTERNATIVE FOOD NETWORK*" OR "SHORT FOOD SUPPLY CHAIN*" OR "LOCAL* FOOD SYSTEM*") AND ("GOVERNANCE" OR "SOCIAL INNOVATION" OR "PROXIMITY" OR "LOGISTIC*" OR "FOOD HUB*" OR “AGROECOLOG*” OR “COOPERAT*” OR "UPSCAL*" OR "DELIVERY" OR "SDG"). Query performed in WOS-SCOPUS-SCIELO. Data retrieved by all members of the ALISOS Research Project - https://proyectoalisos.com/

[EN]The dataset contents the supplementary bibliographical references supplied by principal investigators of the research project

[EN]The dataset contents the records retrieved and selected after bibliographical queries about spatial analysis of sustainable food networks.Query:("ALTERNATIVE FOOD NETWORK*" OR "SHORT FOOD SUPPLY CHAIN*" OR "LOCAL* FOOD SYSTEM*") AND ("ACCESSIBILIT*" OR "CITY-REGION" OR "FOODSHED" OR “SPATIAL MODEL*” OR "BIOREGION" OR "*PRINT"). Query performed in WOS-SCOPUS-SCIELO. Data retrieved by all members of the ALISOS Research Project - https://proyectoalisos.com/

[EN]The dataset contents the records retrieved and selected after bibliographical queries about public policies and sustainable food networks. Query: ("ALTERNATIVE FOOD NETWORK*" OR "SHORT FOOD SUPPLY CHAIN*" OR "LOCAL* FOOD SYSTEM*") AND ("POLICY LAB" OR "URBAN FOOD POLIC*" OR "MILAN" OR "RURAL DEVELOPMENT PROGRAM*" OR "PUBLIC POLIC*"). Query performed in WOS-SCOPUS-SCIELO. Data retrieved by all members of the ALISOS Research Project - https://proyectoalisos.com/

[EN]The dataset contents the script for interviewing public / private stakeholders committed with sustainable food networks. This script was developed with contributions from all members of the ALISOS Research Project - https://proyectoalisos.com/

[EN]The dataset contents the script for interviewing representatives, managers, or owners of sustainable food networks. This script was developed with contributions from all members of the ALISOS Research Project - https://proyectoalisos.com/

[EN]The dataset contents and inventory and characteristics of sustainable food networks in the regions of Castilla y León and Madrid (Spain).Data retrieved by all members of the ALISOS Research Project - https://proyectoalisos.com/