BUSCADOR RECOLECTA

We have previously shown that an HIV vaccine regimen including three doses of HIV-modified vaccinia virus Ankara vector expressing HIV-1 antigens from clade B (MVA-B) was safe and elicited moderate and durable (1 year) T-cell and antibody responses in 75% and 95% of HIV-negative volunteers (n = 24), respectively (RISVAC02 study). Here, we describe the long-term durability of vaccine-induced responses and the safety and immunogenicity of an additional MVA-B boost., Peer reviewed

Poster XVIII SIEBM, Durante 2010 y 2011 se llevaron a cabo varias campañas multidisciplinares en el Cañón de Avilés y el banco de Galicia para estudiar sus hábitats y especies bajo el proyecto LIFE+ INDEMARES. Concretamente, este trabajo se centró en la macrofauna endobentónica de fondos blandos de esta montaña submarina y del complejo de cañones. Se realizaron muestreos cuantitativos de la macroinfauna bentónica con una draga box corer, tras confirmar la presencia de tipos sedimentarios blandos en las estaciones seleccionadas. Con esta draga de fango se obtuvieron muestras para el estudio faunístico y para el estudio granulométrico. El programa de muestreo en el banco de Galicia comprendió un total de 28 estaciones, entre 683 y 2274 m, mientras que en el Cañón de Avilés se muestrearon un total de 57 estaciones entre 83 y 1881 m. Los resultados de los diferentes análisis granulométricos revelaron que los sedimentos del banco de Galicia están compuestos principalmente por arenas medias con bajo contenido orgánico, en tanto que en el Cañón de Avilés el tamaño de grano decreció con la profundidad, desde arenas muy gruesas hasta fango. A nivel faunístico, en el banco de Galicia se observaron menores valores de abundancia y de diversidad (abundancia media por estación: 554 ind/m2, rango: 67-1478 ind/m2; 182 especies, rango: 6-49 especies; diversidad: 2.5-5.1) que en el Cañón de Avilés (abundancia media por estación: 994 ind/m2, rango: 544-2811 ind/m2; 505 especies, rango: 29-81 especies; diversidad: 4.5-5.8). Los poliquetos fueron el grupo faunístico dominante en ambas zonas, tanto en abundancia como en número de especies. Concretamente en el banco de Galicia los poliquetos fueron seguidos en abundancia por los moluscos, equinodermos, crustáceos y el grupo Otros, mientras que en el cañón de Avilés a los poliquetos le siguieron los crustáceos, moluscos, equinodermos, y el grupo Otros. Once familias registraron más del 50% de los individuos en la montaña submarina: los poliquetos Spionidae, Syllidae, Ampharetidae, Poecilochaetidae, Cirratulidae, Paraonidae, Glyceridae y Sabeliidae, los bivalvos Limopsidae y Thyasiridae, y las ofiuras Ophiacanthidae. En el cañón de Avilés fueron trece las familias que alcanzaron más del 50% de dominancia: los poliquetos Spionidae, Paraonidae, Ampharetidae, Sabellidae, Capitellidae, Syllidae, Maldanidae, Onuphidae y Cirratulidae, los bivalvos Bivalvia spp. y Thyasiridae, los nermetinos, y los equinoideos. A nivel específico, las especies más abundantes del banco de Galicia fueron los poliquetos Aurospio dibranchiata, Poecilochaetus sp., Glycera lapidum y Palposyllis prosostoma, junto con los bivalvos Limopsis cristata y Thyasira succisa, mientras que en el cañón de Avilés las especies dominantes fueron los poliquetos Pterolysippe vanelli y Levinsenia flava, los nemertinos, los bivalvos (Bivalvia indet.), los equinoideos (Echinoidea indet.) y el sipuncúlido Onchnesoma steenstrupii steenstrupii. A nivel de comunidades, en el Banco de Galicia los análisis multivariantes distinguieron claramente las estaciones más someras de arenas medias, de las estaciones más profundas de arenas finas, siendo la profundidad, el contenido en fango y el tamaño medio de grano los principales factores que estructuraron las comunidades bentónicas asentadas. En el Cañón de Avilés se determinaron también varios grupos de estaciones en las que se distinguían las estaciones someras con altos contenidos en arenas gruesas, las estaciones de profundidad intermedia con altos contenidos en arenas finas, y las estaciones profundas con las granulometrías más finas, siendo la profundidad, el contenido en arena fina y el contenido en fango los factores estructurantes más importantes.

Maitotoxins (MTXs) are among the most potent toxins known. These toxins are produced by epi-benthic dinoflagellates of the genera Gambierdiscus and Fukuyoa and may play a role in causing the symptoms associated with Ciguatera Fish Poisoning. A recent survey revealed that, of the species tested, the newly described species from the Canary Islands, G. excentricus, is one of the most maitotoxic. The goal of the present study was to characterize MTX-related compounds produced by this species. Initially, lysates of cells from two Canary Island G. excentricus strains VGO791 and VGO792 were partially purified by (i) liquid-liquid partitioning between dichloromethane and aqueous methanol followed by (ii) size-exclusion chromatography. Fractions from chromatographic separation were screened for MTX toxicity using both the neuroblastoma neuro-2a (N2a) cytotoxicity and Ca2+ flux functional assays. Fractions containing MTX activity were analyzed using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) to pinpoint potential MTX analogs. Subsequent non-targeted HRMS analysis permitted the identification of a novel MTX analog, maitotoxin-4 (MTX4, accurate mono-isotopic mass of 3292.4860 Da, as free acid form) in the most toxic fractions. HRMS/MS spectra of MTX4 as well as of MTX are presented. In addition, crude methanolic extracts of five other strains of G. excentricus and 37 other strains representing one Fukuyoa species and ten species, one ribotype and one undetermined strain/species of Gambierdiscus were screened for the presence of MTXs using low resolution tandem mass spectrometry (LRMS/MS). This targeted analysis indicated the original maitotoxin (MTX) was only present in one strain (G. australes S080911_1). Putative maitotoxin-2 (p-MTX2) and maitotoxin-3 (p-MTX3) were identified in several other species, but confirmation was not possible because of the lack of reference material. Maitotoxin-4 was detected in all seven strains of G. excentricus examined, independently of their origin (Brazil, Canary Islands and Caribbean), and not detected in any other species. MTX4 may therefore serve as a biomarker for the highly toxic G. excentricus in the Atlantic area.

Maitotoxins (MTXs) are among the most potent toxins known. These toxins are produced by epi-benthic dinoflagellates of the genera Gambierdiscus and Fukuyoa and may play a role in causing the symptoms associated with Ciguatera Fish Poisoning. A recent survey revealed that, of the species tested, the newly described species from the Canary Islands, G. excentricus, is one of the most maitotoxic. The goal of the present study was to characterize MTX-related compounds produced by this species. Initially, lysates of cells from two Canary Island G. excentricus strains VGO791 and VGO792 were partially purified by (i) liquid-liquid partitioning between dichloromethane and aqueous methanol followed by (ii) size-exclusion chromatography. Fractions from chromatographic separation were screened for MTX toxicity using both the neuroblastoma neuro-2a (N2a) cytotoxicity and Ca2+ flux functional assays. Fractions containing MTX activity were analyzed using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) to pinpoint potential MTX analogs. Subsequent non-targeted HRMS analysis permitted the identification of a novel MTX analog, maitotoxin-4 (MTX4, accurate mono-isotopic mass of 3292.4860 Da, as free acid form) in the most toxic fractions. HRMS/MS spectra of MTX4 as well as of MTX are presented. In addition, crude methanolic extracts of five other strains of G. excentricus and 37 other strains representing one Fukuyoa species and ten species, one ribotype and one undetermined strain/species of Gambierdiscus were screened for the presence of MTXs using low resolution tandem mass spectrometry (LRMS/MS). This targeted analysis indicated the original maitotoxin (MTX) was only present in one strain (G. australes S080911_1). Putative maitotoxin-2 (p-MTX2) and maitotoxin-3 (p-MTX3) were identified in several other species, but confirmation was not possible because of the lack of reference material. Maitotoxin-4 was detected in all seven strains of G. excentricus examined, independently of their origin (Brazil, Canary Islands and Caribbean), and not detected in any other species. MTX4 may therefore serve as a biomarker for the highly toxic G. excentricus in the Atlantic area.

El Mar del Norte alberga importantes bancos de pesca. Es un ecosistema diverso en el que se producen complejas interacciones en la cadena trófica que han sido objeto de estudio por un grupo de científicos de distintos países entre los que se encuentra Marcos Llope, del Instituto Español de Oceanografía. Con él hablamos en nuestro viaje de hoy.

Situación del stock de la sardina

Grup de recerca Tradumàtica, Entrevista a Ràdiu RAI Sardigna sobre l'aparició del primer traductor automàtic per a la llengua sarda (www.apertium.org).

Emès el divendres 2 de juny de 2017 a les 22:15h, Duració: 001710, En la secció de ciència de 'La tarda de Barcelona', de betevé 91.0 fm, l'Òscar Montero ens explica per què, des del segle XIX, Barcelona ha sigut capdavantera en el control alimentari de la carn pel consum humà. Principalment, va ser a conseqüència de la triquinosi. Malgrat l'avenç de la ciència, la zoonosi amenaça una i altra vegada amb noves malalties. En parlem amb José Manuel Gutiérrez, professor de la UAB i expert en veterinària.

The main purpose of this macro-study is to shed light on the broad impact of books. For this purpose, the impact a very large collection of books (more than 200,000) has been analysed by using PlumX, an analytical tool providing a great number of different metrics provided by various tools. Furthermore, the study focuses on the evolution of the most significant measures and indicators over time. The results show usage counts in comparison to the other metrics are quantitatively predominant. Catalogue holdings and reviews represent a book’s most characteristic measures deriving from its increased level of impact in relation to prior results. Our results also corroborate the long half-life of books within the scope of all metrics, excluding views and social media. Despite of some disadvantages, PlumX has proved to be a very helpful and promising tool for assessing the broad impact of books, especially because of how easy it is to enter the ISBN directly as well as its algorithm to aggregate all the data generated by the different ISBN variations.

With the goal of observing the employment history of given individuals, a database was built from the longitudinal data files of the Survey on Living Conditions between 2007-2010 and 2008-2011. Only individuals with continuous presence in the data during the four years were included. Individuals under 16 and over 65 were excluded, as well as the population of the autonomous cities of Ceuta and Melilla. The longitudinal database includes 34,692 observations from 9,003 individuals in 17 regions during the financial crisis of 2007-2011 in Spain., This work was supported by the Health Department. Andalusian Board. 2013-2015 under Grant PI-0682-2012