BUSCADOR RECOLECTA

Response surfaces and contour plots of all optimized methods (Figures S1–S3) and GC-QToF-MS chromatogram of the individual soy by-products (Figure S4)., Peer reviewed

Supplementary figures., Peer reviewed

Supplementary data 1., Peer reviewed

The supporting information: Table S1: Analysis of variance for TPC for response surface modeling showing linear, quadratic and interaction relations and coefficients for model prediction. Table S2: Analysis of variance for AChE for response surface modeling showing linear, quadratic and interaction relations and coefficients for model prediction. Table S3: Analysis of variance for LOX for response surface modeling showing linear, quadratic and interaction relations and coefficients for model prediction. Table S4: Analysis of variance for ROS for response surface modeling showing linear, quadratic and interaction relations and coefficients for model prediction. Table S5: PLE conditions, desirability, predicted response values at the optimum predicted by the model and experimental response values for the selected optimum (50% ethanol at 180 °C). Table S6: Multiple response optimization. Table S7: MS1 (m/z) and RT (min) of the internal standards used. Table S8: Abundance levels of tentatively identified metabolites in thinned peach PLE extracts. Figure S1: Response surfaces for each response variable and their corresponding standardized Pareto charts: A, TPC (mg GAE/mL); B, IC50 AChE (µg/mL); C, IC50 LOX (µg/mL); D, IC50 ROS (µg/mL). Figure S2: Desirability response surface used to optimize all response variables., Peer reviewed

The supporting information: Figure S1: Full MS spectra and structures of coumarins compounds. A: 2-nor-1,2-secolycoserone, B: Lycoserone, C: 6,11′-dihydroxy-1′ H-lycoserone, and D: 9-reduced-10′,11′-dehydro-1′-H-lycoserone, Figure S2: 1H NMR experiment for compound 1, Figure S3: 13C NMR experiment for compound 1, Figure S4: DEPT 13C NMR experiment for compound 1, Figure S5: 1H NMR experiment for compound 2, Figure S6: 13C NMR experiment for compound 2, Figure S7: DEPT 13C NMR experiment for compound 2, Figure S8: Molecular structure of the 2-nor-1,2-secolycoserone and ent-labda-8,13-E-diene-15-ol, Table S1: 1H NMR (500 MHz, CDCl3) experimental data for compound 1, Table S2: 13C NMR (126 MHz, CDCl3) experimental data for compound 1, Table S3: 1H NMR (500 MHz, CDCl3) experimental data for compound 2, Table S4: 13C NMR (126 MHz, CDCl3) experimental data for compound 2, Video S1: Live-cell imaging of HeLa cells exposed to compound 1 (80 µM), Video S2: Live-cell imaging of HeLa cells exposed to compound 2 (50 µM)., Peer reviewed

Supplementary data 1., Peer reviewed

Supplementary data 1: Figure S1. MALDI-TOF-MS profiling of a) milk and milk-colorant; b) casein and casein-colorant; c) whey and whey-colorant (5000–30,000 Da). Figure S2. Reaction of genipin and glycine to form the monomer and since two monomers, the dimer formation., Peer reviewed

Fridericia chica (Bonpl.) L.G. Lohmann (synonym Arrabidaea chica Verlot) (Bignoniaceae) is widely used in folk medicine in Brazil for health benefits. Considering overcoming pitfalls of scaling up production of plant extracts, herein the effects of N2 atomizing gas in spray-drying process of standardized plant extract production is reported. Samples were monitored by in vitro antioxidant activity and microbiological evaluation. The type of gas used did not interfere in the product’s color. However, the drying atmosphere influenced 3-deoxyanthocyanines content when using air as atomizing gas, decreasing carajurin (37.5%) content with concomitant increase in luteolin yield (24.1%). Both drying processes preserved the samples’ pharmacological profile. The antioxidant activity was also similar IC50 (445.60 ± 12.73 and 466.99 ± 46.11). In the cell migration test with HaCaT cells, the extract dried under air flow (5 μg/mL) promoted wound closure by 78% (12 hours) whereas the extract dried using N2 flow promoted 49% (12 hours), with 98% closure (12 hours) for the positive control. The antimicrobial evaluation for Staphylococcus aureus did not differ with the different drying atmospheres, with MIC (minimum inhibitory concentration) at 0.39 mg/mL. Therefore, the drying process reported herein did not interfere with the outcome of the biological activity., Peer reviewed

Supplementary material: Supplementary captions: Figure S1 – Kinetic behavior of the extraction yield (g 100g-1) of supercritical fluid extracts from Pereskia aculeate leaves. Conditions: 40 °C, 25 MPa and 5 SLPM CO2 flow rate. Figure S2 – Kinetic behavior of the extraction yield (g 100g-1) of gas-expanded liquid extraction (GXL EtOH 25%, GXL EtOH 45%, GXL EtOH 75%) and pressurized liquid extraction (PLE EtOH 100%) from Pereskia aculeate leaves. Conditions: 40 °C, 7 MPa and 4 mL min-1. Figure S3 – Global yield (%) from subcritical water extractions (SW) at 5, 10, 15, 20 and 30 min and conventional alkaline extraction (CA) at 45 min of Pereskia aculeate leaves. Conditions: 80°C and 10.5 MPa., Peer reviewed

Supplementary Information: Fig. 1S. Standardized Pareto chart (left) for antioxidant activity (TEAC) and its corresponding response surfaces (right) of D. antarctica extracts by PLE as a function of temperature and solvent (% water: ethanol). The ± signs are interpreted in the Pareto graph according to the area of the significance of factor or interaction in the experimental design. Table S1. Regression coefficients for Extraction yield, DPPH, TEAC, and AchE activity in their original units and statistics for the fit obtained by multiple linear regression., Peer reviewed