BUSCADOR RECOLECTA

We have previously shown that an HIV vaccine regimen including three doses of HIV-modified vaccinia virus Ankara vector expressing HIV-1 antigens from clade B (MVA-B) was safe and elicited moderate and durable (1 year) T-cell and antibody responses in 75% and 95% of HIV-negative volunteers (n = 24), respectively (RISVAC02 study). Here, we describe the long-term durability of vaccine-induced responses and the safety and immunogenicity of an additional MVA-B boost., Peer reviewed

Maitotoxins (MTXs) are among the most potent toxins known. These toxins are produced by epi-benthic dinoflagellates of the genera Gambierdiscus and Fukuyoa and may play a role in causing the symptoms associated with Ciguatera Fish Poisoning. A recent survey revealed that, of the species tested, the newly described species from the Canary Islands, G. excentricus, is one of the most maitotoxic. The goal of the present study was to characterize MTX-related compounds produced by this species. Initially, lysates of cells from two Canary Island G. excentricus strains VGO791 and VGO792 were partially purified by (i) liquid-liquid partitioning between dichloromethane and aqueous methanol followed by (ii) size-exclusion chromatography. Fractions from chromatographic separation were screened for MTX toxicity using both the neuroblastoma neuro-2a (N2a) cytotoxicity and Ca2+ flux functional assays. Fractions containing MTX activity were analyzed using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) to pinpoint potential MTX analogs. Subsequent non-targeted HRMS analysis permitted the identification of a novel MTX analog, maitotoxin-4 (MTX4, accurate mono-isotopic mass of 3292.4860 Da, as free acid form) in the most toxic fractions. HRMS/MS spectra of MTX4 as well as of MTX are presented. In addition, crude methanolic extracts of five other strains of G. excentricus and 37 other strains representing one Fukuyoa species and ten species, one ribotype and one undetermined strain/species of Gambierdiscus were screened for the presence of MTXs using low resolution tandem mass spectrometry (LRMS/MS). This targeted analysis indicated the original maitotoxin (MTX) was only present in one strain (G. australes S080911_1). Putative maitotoxin-2 (p-MTX2) and maitotoxin-3 (p-MTX3) were identified in several other species, but confirmation was not possible because of the lack of reference material. Maitotoxin-4 was detected in all seven strains of G. excentricus examined, independently of their origin (Brazil, Canary Islands and Caribbean), and not detected in any other species. MTX4 may therefore serve as a biomarker for the highly toxic G. excentricus in the Atlantic area.

Maitotoxins (MTXs) are among the most potent toxins known. These toxins are produced by epi-benthic dinoflagellates of the genera Gambierdiscus and Fukuyoa and may play a role in causing the symptoms associated with Ciguatera Fish Poisoning. A recent survey revealed that, of the species tested, the newly described species from the Canary Islands, G. excentricus, is one of the most maitotoxic. The goal of the present study was to characterize MTX-related compounds produced by this species. Initially, lysates of cells from two Canary Island G. excentricus strains VGO791 and VGO792 were partially purified by (i) liquid-liquid partitioning between dichloromethane and aqueous methanol followed by (ii) size-exclusion chromatography. Fractions from chromatographic separation were screened for MTX toxicity using both the neuroblastoma neuro-2a (N2a) cytotoxicity and Ca2+ flux functional assays. Fractions containing MTX activity were analyzed using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) to pinpoint potential MTX analogs. Subsequent non-targeted HRMS analysis permitted the identification of a novel MTX analog, maitotoxin-4 (MTX4, accurate mono-isotopic mass of 3292.4860 Da, as free acid form) in the most toxic fractions. HRMS/MS spectra of MTX4 as well as of MTX are presented. In addition, crude methanolic extracts of five other strains of G. excentricus and 37 other strains representing one Fukuyoa species and ten species, one ribotype and one undetermined strain/species of Gambierdiscus were screened for the presence of MTXs using low resolution tandem mass spectrometry (LRMS/MS). This targeted analysis indicated the original maitotoxin (MTX) was only present in one strain (G. australes S080911_1). Putative maitotoxin-2 (p-MTX2) and maitotoxin-3 (p-MTX3) were identified in several other species, but confirmation was not possible because of the lack of reference material. Maitotoxin-4 was detected in all seven strains of G. excentricus examined, independently of their origin (Brazil, Canary Islands and Caribbean), and not detected in any other species. MTX4 may therefore serve as a biomarker for the highly toxic G. excentricus in the Atlantic area.

El Mar del Norte alberga importantes bancos de pesca. Es un ecosistema diverso en el que se producen complejas interacciones en la cadena trófica que han sido objeto de estudio por un grupo de científicos de distintos países entre los que se encuentra Marcos Llope, del Instituto Español de Oceanografía. Con él hablamos en nuestro viaje de hoy.

[1] Madrid. Convento de Trinitarias.- [2] Madrid. (Paseo de Extremadura): Bloque de viviendas.- [3] Aravaca: Calle de las Flores.- [4] Aravaca: Viviendas.- [5] Brunete: Torre de la iglesia.- [6] Brunete: Una calle.- [7] Brunete: calle de los Mártires.- [8] Guadarrama: Plaza Mayor.- [9] Guadarrama: Casa-Cuartel de la Guardia Civil.- [10] Las Rozas: Parador y viviendas.- [11] Las Rozas: Casa-Cuartel de la Guardia Civil.- [12] Las Rozas: Plaza Mayor.- [13] Sigüenza (Guadalajara): Catedral.- [14] Toledo: bloque de viviendas en la Vega Baja.- [15] Todelo: bloque de viviendas en la Vega Baja.- [16] Toledo: Gobierno Civil y viviendas en Zocodover.- [17] Viver (Castellón): bloque de viviendas para labrador.- [18] Jérica (Castellón: Viviendas.- [19] Chilches (Castellón): nuevas viviendas.- [20] Sagunto (Valencia): viviendas para empleados de Altos Hornos.- [21] El Grao (Valencia): bloque de viviendas..- [22] El Grao (Valencia): bloque de viviendas.- [23] Potes (Santander): Hospital Municipal.- [24] Potes (Santander): centro cívico.- [25] Eibar (Guipúzcoa): bloque de viviendas (a la derecha) y nuevo Mercado (a la izquierda).- [26] Eibar (Guipúzcoa): bloque de viviendas (a la derecha) y nuevo Mercado (a la izquierda).- [27] Lezama (Vizcaya): grupo escolar.- [28] Guernica (Vizcaya): Artekale.- [29] Guernica (Vizcaya): Nuevo ayuntamiento.- [30] Guernica (Vizcaya): jardines y Artekale.- [31] Guernica (Vizcaya): Plaza de España con la Escuela de Arte y Oficios.- [32] Guernica (Vizcaya): urbanización.- [33] [Conjunto de casas]

Lámina perteneciente a "Preceptos generales sobre las operaciones de los partos". Disponible en http://hdl.handle.net/10396/531

Lámina perteneciente a "Preceptos generales sobre las operaciones de los partos". Disponible en http://hdl.handle.net/10396/531

Lámina perteneciente a "Preceptos generales sobre las operaciones de los partos". Disponible en http://hdl.handle.net/10396/531

Lámina perteneciente a "Preceptos generales sobre las operaciones de los partos". Disponible en http://hdl.handle.net/10396/531

Lámina perteneciente a "Preceptos generales sobre las operaciones de los partos". Disponible en http://hdl.handle.net/10396/531