Resultados de investigación

8 files, Dataset of "The Unaccounted Oceanic Sink of Anthropogenic Nitrous Oxide and its Relationship with Anthropogenic Carbon Dioxide" Following files are provided: - column_inventory_cant_molm2_n2oant_mmolm2.csv: Stands for the integrated values of Cant and N2Oant for each method and for the full water column in the WOA 1deg x 1deg grid - 3D_global_inventory_cant_n2oant.nc: Stands for the concentration values of Cant and N2Oant for each method and for the full WOA 1deg x 1deg x 33 levels grid - TTD_ files: mat files for cant and n2oant for the Glodapv2 (2016) dataset, and for Atlantic, Pacific and Indian Ocean, Agencia Estatal de Investigación, BOCATS2, funded by MICIU/AEI/10.13039/501100011033, PID2019‐104279GB‐C21; Agencia Estatal de Investigación, PTA 2019 funded by MCIN/AEI/10.13039/501100011033 PTA2019-017983-I; European Commission, Euro GO-SHIP – Euro GO-SHIP: developing a Research Infrastructure concept to support European hydrography, Peer reviewed

5 files, Global change and urbanisation profoundly alter wildlife habitats, driving native animals into novel habitats while increasing the co-occurrence between native and invasive species. Host-microbiome associations are shaped by host traits and environmental features, but little is known about their plasticity in co-occurring native and invasive species across urban-rural gradients. Here, we explored gut microbiomes of four sympatric small mammal species along an urban-rural ecotone in Borneo, one of the planet's oldest rainforest regions experiencing recent urban expansion. Host species identity was the strongest determinant of microbiome composition, while land use and spatial proximity shaped microbiome similarity within and among the three rat species. The urban-dwelling rat Rattus rattus had a microbiome composition more similar to that of the native, urban-adapted rat Sundamys muelleri (R. rattus’ strongest environmental niche overlap), than to the closely related urban-dwelling R. norvegicus. The urban-dwelling shrew Suncus murinus presented the most distinct microbiome. The microbiome of R. norvegicus was the most sensitive to land use intensity, exhibiting significant alterations in composition and bacterial abundance across the ecotone. Our findings suggest that environmental niche overlap among native and invasive species promotes similar gut microbiomes. Even for omnivorous urban-dwellers with a worldwide distribution like R. norvegicus, gut microbiomes may change across fine-scale environmental gradients. Future research needs to confirm whether land use intensity can be a strong selective force on mammalian gut microbiomes, influencing the way in which native and invasive species are able to exploit novel environments, Peer reviewed

384-assay-mouse+comparison.zip 384-assay-sheep.zip ALIGNMENTS.zip common-genes.txt COUNTS.zip DE_ANALYSIS.zip FASTQ_RAW.zip Gene-count-express.xlsx Metrics.xlsx mouse-vs-sheep-EC50.sav mouse-vs-sheep-EC50.spv Organoids-growth.zip QC.zip QIAXCEL_RESULTS.zip SigDOWN.txt SigUP.txt venn-data.xlsx Z'-mouse.zip Z'-sheep.zip, Peer reviewed

Mycoplasma motility in the presence of antibodies., In embryos, epithelial sealing proceeds with progressive zipping eventually leading to a scar-free epithelium and ensuring the assembly of body segments in insects and neural tube in mammals. How zipping is mechanically controlled to promote tissue fusion on long distances, remains unclear. Combining physical modeling with genetic and mechanical perturbations, we reveal the existence of a transient contractile seam that generates forces to reduce the zipping angle by force balance, consequently promoting epidermal sealing during Drosophila embryogenesis. The seam is formed by the adhesion of two tissues, the epidermis and amnioserosa, and is stabilized by the tensions generated by the segment boundaries. Once a segment is zipped, the seam disassembles concurrently with the inactivation of the Jun kinase pathway. Thus, we show that epithelial sealing is promoted by a transient actomyosin contractile seam allowing sequential segment assembly., Peer reviewed

El acceso completo al dataset estará disponible el 2025-09-06 en https://hdl.handle.net/11441/162315, [Background] Antibiotics could modify multidrug-resistant microorganisms (MDRO) populations in urban wastewater. Water treatment plays a major role in their control. This study aimed to explain the characteristics of multidrug-resistant Gram-negative bacteria in the wastewater of a city in southern Spain, the reduction achieved by treatment and the influence of human antibiotic prescriptions., [Methods] We collected 24-hour composite water samples from four wastewater treatment plants (WWTPs) over one year (2021-2022) and cultured them quantitatively. All acquired ESBL/carbapenemase isolates were sequenced and genomes were compared with those of clinical isolates. Linear mixed model regression analyses were performed to assess the WWTP effect and the association with human antibiotic prescriptions., [Findings] Aeromonas predominated among the MDROs and KPC-2 was the main carbapenemase detected in the influents. The 44% and 30% of E. coli and K. pneumoniae influent isolates, respectively, belonged to high-risk clones; 10·6% of Enterobacteriaceae could be matched to clinical isolates and a strain from a current hospital outbreak was found in raw samples. WWTPs reduced ESBL and carbapenemase producers by 1·4-log and 1·8-log, respectively. New MDROs and persistence of certain strains were detected in effluents. Quinolone and cephalosporin prescriptions, flow-rate and population density were associated with higher OXA-48 producer counts., [Interpretation] Despite the reduction, additional technologies should be implemented in WWTPs receiving hospital discharges. Due to the predominance of environmental species, culture and metagenomic approaches should be combined to differentiate human and sewages origin for antibiotic resistance monitoring. Antibiotic stewardship could be a strategy to reduce the release of MDROs., Junta de Andalucía. Consejería de Universidad, Investigación e Innovación Fundación Francisco Soria Melguizo, Peer reviewed

An active Nap complex cycles between “open” and “closed” states, with the binding site to sialic oligosaccharides becoming alternatively accessible and inaccessible for binding. As shown in this work, during the nap cycle, structural rearrangements are experienced mainly by the C-domain of P1 (brown), with hinge rotations of about 175°, while the ectodomains of P40/P90 (pink) remain mostly unchanged. The wide movement of the C-domain from P1 drags, by contiguity, transmembrane helices thus reaching the internal parts of the TO (represented here simply as a line of elastic (red) and rigid (blue) components with possible displacements shown as black arrows). Binding of antibody P1/MCA (green) to the C-domain of P1 halts the cycle, trapping the “closed” conformation of the Nap. The structures known prior to this work are indicated using soft colors., Peer reviewed

a) Transmembrane helices of adhesins from the M. pneumoniae cluster of mycoplasmas contain three highly conserved Engelman motifs (GxxxG sequences), referred as E1 (P140) and E2, E3 (P110) in M. genitalium. b) Mutational analysis, performed in the Engelman motifs of P140 and P110, indicate that motifs E2 and E3 from P110 have important and synergic contributions to adhesion. On the contrary, no effects were observed when E1 from P140 was mutated. c) Changes in adhesion correlate with increasing rates of cells presenting multiple TOs phenotypes., Peer reviewed

a) Ribbon representations, with three 90° apart views, of the complex between the ectodomain of P1 (brown) and the Fab fragment of Mab P1/MCA4 (light and heavy chains in blue and green, respectively). Insets indicate the main interactions between P1 and the Fab. The epitope is located in the C-domain of P1 closed to the mycoplasma membrane, which is also displayed as a reference. b) Two views of the Fab(P1/MCA4) (shown in green) and the extracellular region of the Nap, with P1 (brown) and P40/P90 (pink transparent). In the “open” conformation of the Nap (left panel) the P1/MCA4 epitope, buried at the center of the Nap, is totally inaccessible to antibodies and a Fab placed there would catastrophically clash with the adhesins. Therefore, to expose the P1/MCA4 epitope the C-domain of P1 has to experience an important rearrangement (right panel)., Peer reviewed

a) Representative micrograph of P1-Fab(P1/MCA4) complexes examined by CryoEM. b) Initial classes obtained after 2D classification of 2000 particles that were manually selected. c) Schematic processing overview for the P1-Fab(P1/MCA4) complex structure resolution from particles that were automatically picked from movies; CTF, Constrast transfer function. d) Fourier shell correlation of P1-Fab(P1/MCA4) complex at a final resolution of 2.39Å according to the 0.143 cut-off criteria. e) Final 3D reconstruction map color-coded according to local resolution., Peer reviewed

Lactobacillales, commonly known as lactic acid bacteria (LAB), is an order of Gram-positive, facultatively anaerobic or microaerophilic bacteria characterized by their ability to ferment carbohydrates and produce lactic acid as a major metabolic byproduct. Many species within this group have significant roles in food fermentation, human health, and industrial applications. Here, we report the complete genome sequence of Fructilactobacillus vespulae Mu01, the first sequenced genome of this species. The complete genome sequence of F. vespulae Mu01 is expected to provide valuable insights into the genetics and metabolism of this little-characterized species., This work was partially financed by the Ministerio de Ciencia, Innovación y Universidades, Agencia Estatal de Investigación, Spain, through project PID2020-119630RB-I00., With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2021-001189-S)., Peer reviewed