Resultados de investigación

Additional file 11: Fig. S6. Effects of loss and gain of otd expression in ommatidial structure and facet size in D. melanogaster. Loss of otd causes defects in ommatidia structure. (a-c) Knockdown of otd by expressing UAS-miR-otd with GMR-GAL4 driver in D. melanogaster eye. (a-a’) GMR-GAL. (b-b’) GMR-GAL/ UAS-miR-otd shows a rough eye phenotype due to defects in ommatidia arrangements. (c-c’) GMR-GAL/ UAS-miR-otd phenotype is rescued by co-expressing UAS-otd. (d-d’) otd imutant mitotic clones also show defects in ommatidia size and organisation. (e) Comparison of facet area from eyes of UAS-otd (“+, otd”), ato3’FL-GAL4 (“ato>+) and UAS-otd; ato3’FL-GAL4 (“ato>otd”) female adults. Facet area is significantly larger in the experimental genotype (“ato>otd”) than in the two control genotypes. See main text for details., Deutsche Forschungsgemeinschaft Biotechnology and Biological Sciences Research Council Ministerio de Ciencia, Innovación y Universidades European Research Council, Peer reviewed

Additional file 12: Table S6. ato-Gal4> UAS-otd ommatidia size., Deutsche Forschungsgemeinschaft Biotechnology and Biological Sciences Research Council Ministerio de Ciencia, Innovación y Universidades European Research Council, Peer reviewed

Additional file 13: Fig. S7. Alignment of D. mauritiana TAM16 and D. simulans y, v, f Otd protein sequences., Deutsche Forschungsgemeinschaft Biotechnology and Biological Sciences Research Council Ministerio de Ciencia, Innovación y Universidades European Research Council, Peer reviewed

Additional file 14: Fig. S8. Topological Domain Associated with otd locus in D. melanogaster from http://chorogenome.ie-freiburg.mpg.de/., Deutsche Forschungsgemeinschaft Biotechnology and Biological Sciences Research Council Ministerio de Ciencia, Innovación y Universidades European Research Council, Peer reviewed

Ministerio de Ciencia, Innovación y Universidades European Research Council, Deutsche Forschungsgemeinschaft Biotechnology and Biological Sciences Research Council Ministerio de Ciencia, Innovación y Universidades European Research Council, Peer reviewed

El dataset se puede consultar y descargar en el siguiente enlace https://osf.io/cmq6k/, LC-MS RAW files of Pinus uncinata along an altitudinal gradient. Publication: Rivas-Ubach et al., An outline on the chemical phenotype flexibility of forest species: an eco-metabolomics study of Pinus uncinata along an altitudinal gradient. Perspectives in Plant Ecology and Evolution., Peer reviewed

Additional file 16: Fig. S9. Activity of tested APREs in the otd locus of D. simulans and D. mauritiana. L3 instar eye-antenna imaginal discs from D. melanogaster lines containing D. mauritiana-APRE4-GAL4, D. mauritiana-APRE6-GAL4, D. mauritiana-APRE7-8-GAL4, D. simulans-APRE11-GAL4, D. mauritiana-APRE13-14-GAL4, D. mauritiana-APRE16-17-GAL4, D. mauritiana-APRE19-GAL4 crossed to UAS-GFP and stained with DAPI (grey). Scale bar = 50μm., Deutsche Forschungsgemeinschaft Biotechnology and Biological Sciences Research Council Ministerio de Ciencia, Innovación y Universidades European Research Council, Peer reviewed

Additional file 17: Table S8. TFBM in differential open chromatin regions in otd locus of D. simulans and D. mauritiana., Deutsche Forschungsgemeinschaft Biotechnology and Biological Sciences Research Council Ministerio de Ciencia, Innovación y Universidades European Research Council, Peer reviewed

Additional file 18: Fig. S10. D. melanogaster, D. simulans and D. mauritiana otd-APRE7-8 enhancer activity in 3rd instar larvae eye imaginal discs. (a) D. melanogaster, D. simulans and D. mauritiana otd-APRE7-8 3rd instar larvae eye imaginal discs immunostained with anti-Elav antibody to visualise the progression of ommatidia maturation as proxy for the developmental time. (b) Plot showing the number of GFP-positive ommatidia rows (x-axis) activated by otd-APRE7-8 at different developmental time points (y-axis, developmental points inferred by number of ommatidia rows) for both species. (c) Violin plots showing the distance between MF and the first row of otd-APRE7-8 activity. D. mauritiana otd-APRE7-8 is active earlier during the differentiation of ommatidia., Deutsche Forschungsgemeinschaft Biotechnology and Biological Sciences Research Council Ministerio de Ciencia, Innovación y Universidades European Research Council, Peer reviewed

Additional file 19: Dataset S1. Clustal Omega alignment of the 2.5 kb APRE7-8 region from selected strains of D. mauritiana, D. simulans and D. melanogaster including the sequences used in enhancer reporter constructs for each species (otd-APRE7-8)., Deutsche Forschungsgemeinschaft Biotechnology and Biological Sciences Research Council Ministerio de Ciencia, Innovación y Universidades European Research Council, Peer reviewed