12 pages, 3 tables, 3 figures, Global production of shell calcium carbonate (CaCO3) from bivalve aquaculture amounts about 13.6 million metric tons per year. Shells, traditionally considered a waste of aquaculture activities, have recently acquired an interest under the current framework of zero waste circular economy. Shell CaCO3 is a sustainable biomaterial that could partly replace the presently dominating non-renewable mineral sources in some applications. Although the carbon footprint of powdered CaCO3 production from biological or mineral sources are about the same, the environmental impact is notably different. Furthermore, bivalve CaCO3 contributes to sequester anthropogenic carbon dioxide (CO2) during shell formation, although this is still controversial. Anyway, the integration of bivalve aquaculture in the carbon trading market would demand that shell CaCO3 is employed in activities that sequester their CO2 for prolonged time periods. Ideally, these activities should combine 1) providing the maximum added value to the biomaterial; 2) producing the lowest carbon footprint during their life cycle; and 3) preserving the CaCO3 integrity as long as possible. In this review, we analyse the effect of bivalve consume habits, particularly mussel, on the availability of shells and their possible agricultural, industrial, and environmental engineering applications from these multiple perspectives. Furthermore, global, continental and regional demands of CaCO3 are matched with their respective mussel aquaculture productions in an effort to scale up offer and demand, This work has been funded by the EU H2020 Research and Innovation Programme AquaVitae project (Grant Agreement No 818173), Peer reviewed

1 file, Summary of the data from figures and tables presented in Alonso A.A., X.A. Álvarez-Salgado, L.T. Antelo (2021). Assessing the impact of bivlave aquaculture on the carbon circular economy. Journal of Cleaner Production 279, 123873. DOI: 10.1016/j.jclepro.2020.123873, EU H2020, poject AquaVitae (EU 818173), Peer reviewed

Poster.-- Aquaculture Europe 2021, Funchal, Madeira, 4-7 October, Aquaculture industry is increasingly incorporating principles of circular economy and zero waste to find alternative ingredients for aquafeeds. The reduction of fishmeal and fish oil inclusion is an established principle in contemporary fish feed formulations. Hence, by-products from other industries, such as rendered animal proteins, are progressively being used. Aquaculture production is seeking for functional ingredients that may enhance fish resilience and robustness, in face of the suboptimal conditions that may be imposed by the rearing procedures and new dietary formulations, This project has received funding from the European Union’s Horizon 2020 Research and Innovation programme under Grant Agreement No 818173 and by the Portuguese Foundation for Science and Technology (Ministry of Science and Higher Education, Portugal) through project UIDB/04326/2020 to CCMAR and contract DL 57/2016/CP1361/CT0033 to CA, No

Aquaculture Europe 2021, Funchal, Madeira, 4-7 October, In 2020 world population was projected to increase from 7.8 billion to 9.9 billion by 2050 (http://worldpopulationreview.com). To overcome the stagnation of wild fisheries in supplying seafood for the growing population, Aquaculture will need to address several technical challenges to secure food demand (FAO, 2020). One of the challenges is to expand the number of sustainable raw materials to increase flexibility in the formulation of highly nutritious aquafeeds, This project has received funding from the European Union’s Horizon 2020 Research and Innovation programme under Grant Agreement No 818173 and by the Portuguese Foundation for Science and Technology (Ministry of Science and Higher Education, Portugal) through project UIDB/04326/2020 to CCMAR and contract DL 57/2016/CP1361/CT0033 to CA., No

Aquaculture Europe 2022, International Conference & Exposition, September 27-30 2022, Rimini, Italy, Aquaculture industry is a key animal production sector to secure the future food demand. Expanding the number of raw materials will increase flexibility in the formulation of highly nutritious aquafeeds and the sustainability of the sector. The inclusion of mussel meal originated from mussel aquaculture side-streams in aquafeeds will contribute to the valorisation of aquaculture value-chains and to the circular economy approach in the industry.
The present study aimed to evaluate gilthead seabream (Sparus aurata) juveniles growth performance, feed utilisation, nutrient apparent digestibility, and nitrogen outputs to the environment when fed experimental diets with mussel meal as the main source of marine-derived protein, This project has received funding from the European Union’s Horizon 2020 Research and Innovation programme under Grant Agreement No 818173 and by the Portuguese Foundation for Science and Technology (Ministry of Science and Higher Education, Portugal) through projects UIDB/04326/2020, UIDP/04326/2020, LA/P/0101/2020 to CCMAR and contract DL 57/2016/CP1361/CT0033 to CA, No

Aquaculture Europe 2022, International Conference & Exposition, September 27-30 2022, Rimini, Italy, AquaVitae (AV) is a research and innovation project funded by the EU’s Horizon 2020 program. AV’s overall objective is to introduce new, and expand existing, low trophic species (LTS) products and processes to marine aquaculture value chains across the Atlantic. The value chains that AV focuses on include macroalgae, integrated multi-trophic aquaculture, echinoderms, underutilized shellfish species and low trophic finfish species. Moreover, AV includes analysis of value chains, market development and profitability, and other biological and socioeconomic aspects, including sustainability, environmental monitoring, and risk assessment of LTS aquaculture, This research is supported by the European Union’s H2020 Research and Innovation program within the collaborative project “AQUAVITAE– New species, processes and products contributing to increased production and improved sustainability in emerging low trophic, and existing low and high trophic aquaculture value chains in the Atlantic” under Grant Agreement No. 818173, No

17 pages, 9 figures, 2 tables.-- Under a Creative Commons license, Interest on the potential CO2 sequestration of marine bivalve aquaculture has increased during the last decade. However, there is still some controversy about which biological processes are involved and how to estimate their contribution to the carbon footprint of bivalve aquaculture. This work considers the dissolved inorganic carbon, CO2 and alkalinity fluxes linked to flesh and shell growth, calcification, respiration, faeces egestion, and ammonia excretion, accounting also for the RDOC production associated to these processes. We have developed an algorithm for a dynamic estimation of these fluxes based on a net production DEB growth model for mussels. The resulting model has been implemented in Python to create a toolbox with a graphical user interface. This toolbox allows the selection of different culture strategies, in terms of seeding date, seed size and culture length, and consequently analyzes the carbon footprint and impact on the carbonate chemistry of seawater of aquaculture management, This study has been funded by EU H2020 project Aquavitae (EU 818173). MP also acknowledges support from grant FJC2019–041397-I funded by MCIN/AEI/ 10.13039/501100011033, Peer reviewed

13 pages, 3 figures, 4 tables, The aquaculture industry must continue to reduce its reliance on finite marine ingredients and promote biocircularity to enhance sustainability. This study evaluated the effects of no-fishmeal (FM) diets and fish protein hydrolysates (FPHs) on the growth performance, antioxidant status, and immune responses of gilthead seabream (Sparus aurata). Following established conditions, two FPHs were prepared from the enzymatic hydrolysis of discards from whole-body blue whiting (Micromesistius poutassou) and gurnard heads (Trigla spp.); the former contained a higher proportion of large peptides (LPs), while the latter had more small peptides (SPs). Four isoproteic (48%) and isolipic (16%) diets were tested: a commercial (COM)-like diet with 35% FM, 10% poultry meal, and 5% soy protein concentrate; a FUTURE (FUT) diet, without FM or soy protein concentrate, containing 25% poultry meal; and two FUT diets supplemented with FPH (FUTLP and FUTSP). Gilthead seabream (initial weight ± 8.0 g) was distributed into 500 L tanks at an initial density of 1.4 kg m−3 and fed the experimental diets to apparent satiety for 8 weeks. Sampling was performed at the end of the growth trial, followed by a digestibility trial. Nutrient and energy digestibilities were significantly lower in the FUT than in the COM diet, with protein and energy digestibilities being 7% and 16% lower, respectively, in the FUT treatment, leading to higher nitrogen losses. Growth performance and feed utilization were negatively impacted in the FUT treatment, with weight gain of only 310 ± 33% compared to 482 ± 22% in the COM treatment. Additionally, immune responses in plasma and antioxidant status in the liver were slightly impaired in the FUT treatment. Supplementation of FPH to the FUT diet mitigated or even reversed these negative effects. The results confirmed that including small- and medium-sized peptides in no-FM diets is more beneficial than using larger ones, This project has received funding from the European Union’s Horizon 2020 Research and Innovation Programme (grant agreement no. 818173), the Xunta de Galicia (Grupos con Potencial de Crecemento [IN607B 2021/11]), and Portuguese national funds from Fundação para a Ciência e Tecnologia (projects [UIDB/04326/2020], [UIDP/04326/2020], [LA/P/0101/2020], and contract [DL57/2016/CP1361/CT0033]), Peer reviewed

Aim of study: To evaluate the effects of different fish feeding rates on the growth performance, water quality, and water microbiology in the integrated culture of lebranche mullet (Mugil liza) and whiteleg shrimp (Penaeus vannamei) using biofloc technology.
Area of study: Southern Brazil.
Material and methods: A 46-day experiment was performed to assess four feeding rates (0%, 1%, 2%, and 3% of mullet biomass) with four replicates per treatment. Shrimp were fed according to a feeding table. Eight-hundred L tanks were used for shrimp culture, while 90 L tanks were utilized for mullet culture, employing recirculation between the tanks through a submerged pump (Sarlo-Better 650 L hour-1).
Main results: Water quality variables and water microbiology, evaluated through bacterial counts, were unaffected by the fish feeding rates (p>0.05). Regarding growth performance, while shrimp were unaffected (p>0.05), mullet final mean weight, biomass, daily growth coefficient, and yield significantly increased with higher fish feeding rates (p<0.05). Mullet feed conversion ratio and survival were not influenced by the feeding management (p>0.05). Furthermore, linear regression models for the overall system productivity showed a positive correlation with the fish feeding rate. As the fish feeding rate increased, the yield of the integrated culture system also increased (p<0.05).
Research highlights: These findings emphasize the importance of considering appropriate feeding rates to maximize the productivity and overall performance of integrated aquaculture systems using biofloc technology.