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Interrelating EPS, soluble microbial products and metal solubility in a methanogenic consortium stressed by nickel and cobalt

Digital.CSIC. Repositorio Institucional del CSIC
  • Hasani Zadeh, Parvin
  • Serrano, Antonio
  • Collins, Gavin
  • Fermoso, Fernando G.
8 Páginas.-- 5 Figuras.-- 2 Tablas, The relationships between extracellular polymeric substances (EPS), soluble microbial product production, metal solubility, and methanogenic activity were investigated. The individual, and joint, toxic effects of nickel and cobalt on methanogenic consortia fed with glucose as model substrate were studied using biomethane potential assays. Cobalt was found to be less toxic to methanogens than nickel at each concentration tested, and the combined effects of Ni and Co on methane production in the bimetal experiment was higher than the sum of the effects of each metal alone. The protein content of EPS, and extracellular soluble protein fractions, decreased with increasing concentrations of total metals. Meanwhile, no significant change in response to metal stress was apparent for carbohydrate content of EPS or extracellular soluble carbohydrate. Decreasing protein content of EPS was accompanied by reduced methanogenic activity and an increase in the soluble metal fraction. The strong associations observed between these variables could be due to the critical role of EPS in protecting microbial cells against nickel and cobalt stress, possibly by capturing metal cations through their functional groups, thus reducing metal availability to the microbial cells in the methanogenic consortia underpinning the anaerobic digestion process., This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 861088. Antonio Serrano is grateful to the Economic Transformation, Industry, Knowledge and Universities Department of the Andalucia Autonomous Government for his Emergia fellowship (EMERGIA20_00114)., Peer reviewed
Proyecto: EC/H2020/861088




Trace metal fate in soil after application of digestate originating from the anaerobic digestion of non-source-separated organic fraction of municipal solid waste

Digital.CSIC. Repositorio Institucional del CSIC
  • Baldasso, Veronica
  • Bonet-Garcia, Neus
  • Sayen, Stéphanie
  • Guillon, Emmanuel
  • Frunzo, Luigi
  • Gomes, Carlos A. R.
  • Alves, Maria João
  • Castro, Ricardo
  • Mucha, Ana Paula
  • Almeida, C. Marisa R.
16 Páginas.-- 6 Figuras
Este trabajo es financiado por el proyecto M2ex “Exploiting metal-microbe applications to expand the circular economy”, cuyo Investigador Principal es el Dr. Fernando G. Fermoso (Instituto de la Grasa-CSIC), Introduction: Digestate originating from anaerobic digestion of non-source-separated organic fraction of municipal solid waste (OFMSW) is produced abundantly worldwide and generally discarded in landfills. However, it can be a valuable resource for many bioeconomy strategies as land restoration, only if a consolidated understanding of the contaminants’ presence and behaviour in digestate-amended soil is achieved. This study aimed to investigate the fate of trace metals, namely Zn, Cu, Pb, and Cr found in the digestate, along the soil profile after digestate application on soil, and the influence that other contaminants as pharmaceutical compounds can have on their behaviour in the soil system.

Methods: For that, a 90-day soil column experiment was conducted using a fine loamy sand soil topped with a layer of digestate-amended soil. Digestate-amended soil had a soil to digestate proportion of 14 to 1 (dry weight). Two experimental conditions were tested: soil amended with digestate, and soil amended with digestate spiked with the antidiabetic drug metformin. Soil samples were taken at 4 depths on days 1, 7, 21, 35 and 90, and total trace metals concentrations and fractionation were determined via atomic absorption spectroscopy.

Results: Results showed that Zn, Cu, Pb ad Cr initially present in the digestate were transferred from the digestate-amended soil layer to the underlying soil layer over time, although in low amounts. Nevertheless, no transfer was detected to the deeper soil layers. Trace metals in soil were predominantly in immobile and less bioavailable forms associated with clay and silicate mineral groups, whereas in the digestate-amended soil they were in more bioavailable forms, which could be related to metals’ migration in the soil layers below. Results also show that the presence of metformin had no influence on trace metal behaviour, giving insight also on possible interactions with other potentially present contaminants as microplastics.

Conclusion: The current study showed that OFMSW digestate can be a promising organic nutrient-rich matrix for land restoration even if it may contain high metals’ concentrations because low metal mobility along the soil profile can be expected., This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 861088., Peer reviewed
Proyecto: EC/H2020/861088




M2ex project. Trace metal fate in soil after application of digestate originating from the anaerobic digestion of non-source-separated organic fraction of municipal solid waste [Dataset]

Digital.CSIC. Repositorio Institucional del CSIC
  • Baldasso, Veronica
  • Bonet-Garcia, Neus
  • Sayen, Stéphanie
  • Guillon, Emmanuel
  • Frunzo, Luigi
  • Gomes, Carlos A. R.
  • Alves, Maria João
  • Castro, Ricardo
  • Mucha, Ana Paula
  • Almeida, C. Marisa R.
Exploiting metal-microbe applications to expand the circular economy (M2ex) GA no 861088, 1 Excel file, Digestate originating from anaerobic digestion of non-source-separated organic fraction of municipal solid waste (OFMSW) is produced abundantly worldwide and generally discarded in landfills. However, it can be a valuable resource for many bioeconomy strategies as land restoration, only if a consolidated understanding of the contaminants’ presence and behaviour in digestate-amended soil is achieved. This study aimed to investigate the fate of trace metals, namely Zn, Cu, Pb, and Cr found in the digestate, along the soil profile after digestate application on soil, and the influence that other contaminants as pharmaceutical compounds can have on their behaviour in the soil system. For that, a 90-day soil column experiment was conducted using a fine loamy sand soil topped with a layer of digestate-amended soil. Digestate-amended soil had a soil to digestate proportion of 14 to 1 (dry weight). Two experimental conditions were tested: soil amended with digestate, and soil amended with digestate spiked with the antidiabetic drug metformin. Soil samples were taken at 4 depths on days 1, 7, 21, 35 and 90, and total trace metals concentrations and fractionation was determined via atomic absorption spectroscopy. Results showed that Zn, Cu, Pb ad Cr initially present in the digestate were transferred from the digestate-amended soil layer to the underlying soil layer over time, although in low amounts. Nevertheless, no transfer was detected to the deeper soil layers. Trace metals in soil were predominantly in immobile and less bioavailable forms associated with clay and silicate mineral groups, whereas in the digestate-amended soil they were in more bioavailable forms, which could be related also to metals’ migration in the soil layers below. Results also show that the presence of metformin had no influence on trace metal behaviour, giving insight also on possible interactions with other potentially present contaminants as microplastics. The current study showed that OFMSW digestate can be a promising organic nutrient-rich matrix for land restoration even if it may contain high metals’ concentrations because low metal mobility along the soil profile can be expected., This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 861088., Peer reviewed
Proyecto: EC/H2020/861088




Impacts of metal stress on extracellular microbial products, and potential for selective metal recovery

Digital.CSIC. Repositorio Institucional del CSIC
  • Hasani Zadeh, Parvin
  • Fermoso, Fernando G.
  • Collins, Gavin
  • Serrano, Antonio
  • Mills, Simon
  • Abram, Florence
13 Páginas.-- · Figuras.-- 3 Tablas, Harnessing microbial capabilities for metal recovery from secondary waste sources is an eco-friendly and sustainable approach for the management of metal-containing wastes. Soluble microbial products (SMP) and extracellular polymeric substances (EPS) are the two main groups of extracellular compounds produced by microorganisms in response to metal stress that are of great importance for remediation and recovery of metals. These include various high-, and low, molecular weight components, which serve various functional and structural roles. These compounds often contain functional groups with metal binding potential that can attenuate metal stress by sequestering metal ions, making them less bioavailable. Microorganisms can regulate the content and composition of EPS and SMP in response to metal stress in order to increase the compounds specificity and capacity for metal binding. Thus, EPS and SMP represent ideal candidates for developing technologies for selective metal recovery from complex wastes. To discover highly metal-sorptive compounds with specific metal binding affinity for metal recovery applications, it is necessary to investigate the metal binding affinity of these compounds, especially under metal stressed conditions. In this review we critically reviewed microbial EPS and SMP production as a response to metal stress with a particular emphasis on the metal binding properties of these compounds and their role in altering metal bioavailability. Furthermore, for the first time, we compiled the available data on potential application of these compounds for selective metal recovery from waste streams., This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 861088., Peer reviewed
Proyecto: EC/H2020/861088




Dynamic modelling the effects of ionic strength and ion complexation on trace metal speciation during anaerobic digestion

Digital.CSIC. Repositorio Institucional del CSIC
  • George, Susan
  • Mattei, Maria Rosaria
  • Frunzo, Luigi
  • Esposito, Giovanni
  • van Hullebusch, Eric D
  • Fermoso, Fernando G.
10 Páginas.-- 6 Figuras.-- 2 Tablas, Dosing trace metals into anaerobic digestors is proven to improve biogas production rate and yield by stimulating microorganisms involved in the metabolic pathways. Trace metal effects are governed by metal speciation and bioavailability. Though chemical equilibrium speciation models are well-established and widely used to understand metal speciation, the development of kinetic models considering biological and physicochemical processes has recently gained attention. This work proposes a dynamic model for metal speciation during anaerobic digestion which is based on a system of ordinary differential equations aimed to describe the kinetics of biological, precipitation/dissolution, gas transfer processes and, a system of algebraic equations to define fast ion complexation processes. The model also considers ion activity corrections to define effects of ionic strength. Results from this study shows the inaccuracy in predicting trace metal effects on anaerobic digestion by typical metal speciation models and the significance of considering non-ideal aqueous phase chemistry (ionic strength and ion pairing/complexation) to define speciation and metal labile fractions. Model results show a decrease in metal precipitation and increase in metal dissolved fraction and methane production yield with increase in ionic strength. Capability of the model to dynamically predict trace metal effects on anaerobic digestion under different conditions, like changing dosing conditions and initial iron to sulphide ratio, was also tested and verified. Dosing iron increases methane production and decreases hydrogen sulphide production. However, when iron to sulphide ratio is greater than 1, methane production decreases due to increase in dissolved iron which reaches inhibitory concentration levels., This research is supported by the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No. 861088, Peer reviewed
Proyecto: EC/H2020/861088




Interrelating EPS, soluble microbial products and metal solubility in a methanogenic consortium stressed by nickel and cobalt

Digibug. Repositorio Institucional de la Universidad de Granada
  • Hasani Zadeh, Parvin
  • Serrano Moral, Antonio
The relationships between extracellular polymeric substances (EPS), soluble microbial product production, metal
solubility, and methanogenic activity were investigated. The individual, and joint, toxic effects of nickel and
cobalt on methanogenic consortia fed with glucose as model substrate were studied using biomethane potential
assays. Cobalt was found to be less toxic to methanogens than nickel at each concentration tested, and the
combined effects of Ni and Co on methane production in the bimetal experiment was higher than the sum of the
effects of each metal alone. The protein content of EPS, and extracellular soluble protein fractions, decreased
with increasing concentrations of total metals. Meanwhile, no significant change in response to metal stress was
apparent for carbohydrate content of EPS or extracellular soluble carbohydrate. Decreasing protein content of
EPS was accompanied by reduced methanogenic activity and an increase in the soluble metal fraction. The strong
associations observed between these variables could be due to the critical role of EPS in protecting microbial cells
against nickel and cobalt stress, possibly by capturing metal cations through their functional groups, thus
reducing metal availability to the microbial cells in the methanogenic consortia underpinning the anaerobic
digestion process., European Commission 861088, Economic Transformation, Industry, Knowledge and Universities Department of the Andalucia Autonomous Government EMERGIA20_00114
Proyecto: EC/H2020/861088




Impacts of metal stress on extracellular microbial products, and potential for selective metal recovery

Digibug. Repositorio Institucional de la Universidad de Granada
  • Zadeh, Parvin Hasani
  • Serrano Moral, Antonio
Harnessing microbial capabilities for metal recovery from secondary waste sources is an eco-friendly and sus-
tainable approach for the management of metal-containing wastes. Soluble microbial products (SMP) and
extracellular polymeric substances (EPS) are the two main groups of extracellular compounds produced by
microorganisms in response to metal stress that are of great importance for remediation and recovery of metals.
These include various high-, and low, molecular weight components, which serve various functional and
structural roles. These compounds often contain functional groups with metal binding potential that can
attenuate metal stress by sequestering metal ions, making them less bioavailable. Microorganisms can regulate
the content and composition of EPS and SMP in response to metal stress in order to increase the compounds
specificity and capacity for metal binding. Thus, EPS and SMP represent ideal candidates for developing tech-
nologies for selective metal recovery from complex wastes. To discover highly metal-sorptive compounds with
specific metal binding affinity for metal recovery applications, it is necessary to investigate the metal binding
affinity of these compounds, especially under metal stressed conditions. In this review we critically reviewed
microbial EPS and SMP production as a response to metal stress with a particular emphasis on the metal binding
properties of these compounds and their role in altering metal bioavailability. Furthermore, for the first time, we
compiled the available data on potential application of these compounds for selective metal recovery from waste
streams, European Union’s Horizon 2020 programme Marie Sklodowska-Curie 861088