EVALUACION DE LOS MODELOS ANIMALES Y LA PROTECCION FRENTE A LA ENFERMEDAD DE GLASSER Y EL PAPEL DE LA MICROBIOTA RESPIRATORIA
AGL2013-45662-R
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Nombre agencia financiadora Ministerio de Economía y Competitividad
Acrónimo agencia financiadora MINECO
Programa Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia
Subprograma Subprograma Estatal de Generación del Conocimiento
Convocatoria Retos Investigación: Proyectos de I+D+I
Año convocatoria 2013
Unidad de gestión Dirección General de Investigación Científica y Técnica
Centro beneficiario FUNDACIÓ CENTRE DE RECERCA EN SANITAT ANIMAL (CRESA) / FUNDACIÓN CENTRO DE INVESTIGACIÓN EN SANIDAD ANIMAL (CRESA)
Centro realización SUBPROGRAMA INFECCIONS BACTERIANES I ENDOPARASITARIES, I RESISTENCIA A ANTIMICROBIANS (BACPAR)
Identificador persistente http://dx.doi.org/10.13039/501100003329
Publicaciones
Resultados totales (Incluyendo duplicados): 1
Encontrada(s) 1 página(s)
Encontrada(s) 1 página(s)
Novel blaROB-1-bearing plasmid conferring resistance to β-lactams in Haemophilus parasuis isolates from healthy weaning pigs
Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
- Moleres Apilluelo, Javier
- Santos López, Alfonso
- Lázaro, Isidro
- Labairu, Javier
- Prat, Cristina
- Ardanuy, Carmen
- González Zorn, Bruno
- Aragon, Virginia
- Garmendia García, Juncal
Haemophilus parasuis, the causative agent of Glässer’s disease, is one of the early colonizers of the nasal mucosa of piglets. It is
prevalent in swine herds, and lesions associated with disease are fibrinous polyserositis and bronchopneumonia. Antibiotics are
commonly used in disease control, and resistance to several antibiotics has been described in H. parasuis. Prediction of H. parasuis
virulence is currently limited by our scarce understanding of its pathogenicity. Some genes have been associated with H.
parasuis virulence, such as lsgB and group 1 vtaA, while biofilm growth has been associated with nonvirulent strains. In this
study, 86 H. parasuis nasal isolates from farms that had not had a case of disease for more than 10 years were obtained by sampling
piglets at weaning. Isolates were studied by enterobacterial repetitive intergenic consensus PCR and determination of the
presence of lsgB and group 1 vtaA, biofilm formation, inflammatory cell response, and resistance to antibiotics. As part of the
diversity encountered, a novel 2,661-bp plasmid, named pJMA-1, bearing the blaROB-1 β-lactamase was detected in eight colonizing
strains. pJMA-1 was shown to share a backbone with other small plasmids described in the Pasteurellaceae, to be 100% stable,
and to have a lower biological cost than the previously described plasmid pB1000. pJMA-1 was also found in nine H. parasuis
nasal strains from a separate collection, but it was not detected in isolates from the lesions of animals with Glässer’s disease
or in nontypeable Haemophilus influenzae isolates. Altogether, we show that commensal H. parasuis isolates represent a reservoir
of β-lactam resistance genes which can be transferred to pathogens or other bacteria., J.M. is funded by Ph.D. studentship BES-2013-062644 from the Ministerio de Economía y Competitividad-MINECO, Spain. This work has been
funded by grants from MINECO (SAF2012-31166) and the Departamento Industria Gobierno Navarra (IlQ14064.R12) to J.G., MINECO (AGL2013-45662) to V.A., and EU projects EvoTAR 282004-FP7 and EFFORT 613754-FP7 to B.G.-Z.
prevalent in swine herds, and lesions associated with disease are fibrinous polyserositis and bronchopneumonia. Antibiotics are
commonly used in disease control, and resistance to several antibiotics has been described in H. parasuis. Prediction of H. parasuis
virulence is currently limited by our scarce understanding of its pathogenicity. Some genes have been associated with H.
parasuis virulence, such as lsgB and group 1 vtaA, while biofilm growth has been associated with nonvirulent strains. In this
study, 86 H. parasuis nasal isolates from farms that had not had a case of disease for more than 10 years were obtained by sampling
piglets at weaning. Isolates were studied by enterobacterial repetitive intergenic consensus PCR and determination of the
presence of lsgB and group 1 vtaA, biofilm formation, inflammatory cell response, and resistance to antibiotics. As part of the
diversity encountered, a novel 2,661-bp plasmid, named pJMA-1, bearing the blaROB-1 β-lactamase was detected in eight colonizing
strains. pJMA-1 was shown to share a backbone with other small plasmids described in the Pasteurellaceae, to be 100% stable,
and to have a lower biological cost than the previously described plasmid pB1000. pJMA-1 was also found in nine H. parasuis
nasal strains from a separate collection, but it was not detected in isolates from the lesions of animals with Glässer’s disease
or in nontypeable Haemophilus influenzae isolates. Altogether, we show that commensal H. parasuis isolates represent a reservoir
of β-lactam resistance genes which can be transferred to pathogens or other bacteria., J.M. is funded by Ph.D. studentship BES-2013-062644 from the Ministerio de Economía y Competitividad-MINECO, Spain. This work has been
funded by grants from MINECO (SAF2012-31166) and the Departamento Industria Gobierno Navarra (IlQ14064.R12) to J.G., MINECO (AGL2013-45662) to V.A., and EU projects EvoTAR 282004-FP7 and EFFORT 613754-FP7 to B.G.-Z.