Nombre agencia financiadora Ministerio de Economía y Competitividad
Acrónimo agencia financiadora MINECO
Programa Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia
Subprograma Subprograma Estatal de Generación del Conocimiento
Convocatoria Proyectos de I+D dentro del Subprograma Estatal de Generación del Conocimiento (2014)
Año convocatoria 2014
Unidad de gestión Dirección General de Investigación Científica y Técnica
Identificador persistente


Found(s) 4 result(s)
Found(s) 1 page(s)

Nitric oxide induces the alternative oxidase pathway in Arabidopsis seedlings deprived of inorganic phosphate

Digital.CSIC. Repositorio Institucional del CSIC
  • Royo, Beatriz
  • Morán, José F.
  • Ratcliffe, George
  • Gupta, Kapuganti J.
Phosphate starvation compromises electron fow through the cytochrome pathway of the mitochondrial electron transport chain, and plants commonly respond to phosphate deprivation by increasing fow through the alternative oxidase (AOX). To test whether this response is linked to the increase in nitric oxide (NO) production that also increases under phosphate starvation, Arabidopsis thaliana seedlings were grown for 15 d on media containing either 0 or 1 mM inorganic phosphate. The effects of the phosphate supply on growth, the production of NO, respiration, the AOX level and the production of superoxide were compared for wild-type (WT) seedlings and the nitrate reductase double mutant nia. Phosphate deprivation increased NO production in WT roots, and the AOX level and the capacity of the alternative pathway to consume electrons in WT seedlings; whereas the same treatment failed to stimulate NO production and AOX expression in the nia mutant, and the plants had an altered growth phenotype. The NO donor S-nitrosoglutathione rescued the growth phenotype of the nia mutants under phosphate deprivation to some extent, and it also increased the respiratory capacity of AOX. It is concluded that NO is required for the induction of the AOX pathway when seedlings are grown under phosphate-limiting conditions., This work was supported by research grants (AGL2010-16167; AGL2014-52396-P) from the Spanish Ministry of Economy and Competitiveness (MINECO) to JFM, an FPI fellowship from MINECO (BR), and a Marie Curie Intra-European Fellowship for Career Development within the 7th European Community Framework Programme (KJG and RGR)., Peer Reviewed

Review: Mechanisms of ammonium toxicity and the quest for tolerance

Digital.CSIC. Repositorio Institucional del CSIC
  • Esteban, Raquel
  • Ariz, Idoia
  • Cruz, Cristina
  • Morán, José F.
Ammonium sensitivity of plants is a worldwide problem, constraining crop production. Prolonged application of ammonium as the sole nitrogen source may result in physiological and morphological disorders that lead to decreased plant growth and toxicity. The main causes of ammonium toxicity/tolerance described until now include high ammonium assimilation by plants and/or low sensitivity to external pH acidification. The various ammonium transport-related components, especially the non-electrogenic influx of NH3 (related to the depletion of 15N) and the electrogenic influx of NH4+, may contribute to ammonium accumulation, and therefore to NH3 toxicity. However, this accumulation may be influenced by increasing K+ concentration in the root medium. Recently, new insights have been provided by “omics” studies, leading to a suggested involvement of GDP mannose-pyrophosphorylase in the response pathways of NH4+ stress. In this review, we highlight the cross-talk signaling between nitrate, auxins and NO, and the importance of the connection of the plants’ urea cycle to metabolism of polyamines. Overall, the tolerance and amelioration of ammonium toxicity are outlined to improve the yield of ammonium-grown plants. This review identifies future directions of research, focusing on the putative importance of aquaporins in ammonium influx, and on genes involved in ammonium sensitivity and tolerance., The authors acknowledge the support of research grants AGL2014-52396-P from the Spanish Ministry of Economy and Competitiveness (MINECO) and PTDC/BIA-BEC/099323/2008 and PTDC/BIA-ECS/122214/2010 from the Portuguese Fundação para a Ciência e Tecnologia (FCT). RE received a JAE-Doc-2011-046 fellow from the Spanish CSIC, co-financed by the European Social Fund. IA was supported by a postdoctoral Fellowship from the FCT (SFRH/BPD/904 36/2012)., Peer reviewed

Drought tolerance response of high‐yielding soybean varieties to mild drought: physiological and photochemical adjustments

Digital.CSIC. Repositorio Institucional del CSIC
  • Buezo, Javier
  • Sanz-Sáez, Álvaro
  • Morán, José F.
  • Soba, David
  • Aranjuelo, Iker
  • Esteban, Raquel
Soybean is a crop of agronomic importance that requires adequate watering during its growth to achieve high production. In this study, we determined physiological, photochemical and metabolic differences in five soybean varieties selected from the parental lines of a nested association mapping population during mild drought. These varieties have been described as high yielding (NE3001, HY1; LD01‐5907, HY2) or drought tolerant (PI518751; HYD1; PI398881, HYD2). Nevertheless, there has been little research on the physiological traits that sustain their high productivity under water‐limited conditions. The results indicate that high‐yielding varieties under drought cope with the shortage of water by enhancing their photoprotective defences and invest in growth and productivity, linked to a higher intrinsic water use efficiency. This is the case of the variety N‐3001 (HY1), with a tolerance strategy involving a faster transition into the reproductive stage to avoid the drought period. The present study highlights the role of the physiological and biochemical adjustments of various soybean varieties to cope with water‐limited conditions. Moreover, the obtained results underscore the fact that the high phenotypic plasticity among soybean phenotypes should be exploited to compensate for the low genetic variability of this species when selecting plant productivity in constrained environments., This work was supported by grants from the Spanish Ministry of Science and Innovation and FEDER funds (AGL2014‐52396) and from the Basque Government (UPV/EHU‐GV IT‐1018‐16; IT‐932‐16). J.B. is a holder of a PhD fellowship from the Public University of Navarre. R.E. received a Juan de la Cierva‐incorporación grant (IJCI‐2014‐21452)., Peer reviewed

Selection of grazing-tolerant alfalfa genotypes

Digital.CSIC. Repositorio Institucional del CSIC
  • Rockenbach de Ávila, Mariana
  • Dall´Agnol, M.
  • Morán, José F.
  • Martinelli, José A.
  • Esteban, Raquel
  • Silva, Gerarda da
Trabajo presentado en la 53º Reunião Anual da Sociedade Brasileira de Zootecnia, celebrada en Gramado (Brasil), del 1 al 4 de agosto de 2016, Alfalfa (Medicago sativa L.) is a high quality persistent legume, which can be used as
forage, silage or hay. Alfalfa cultivar Crioula is the most frequently grown and the bestadapted cultivar to Brazil, and it is considered as a hay-type because of its upright habit
and limited tolerance to severe defoliation. Nevertheless, experiments conducted at
Federal University of Rio Grande do Sul (UFRGS) observed variability in characters of
this cultivar such as the morphology of seedlings. Therefore, the objective of this work
was to evaluate new grazing tolerant genotypes of alfalfa by selecting genotypes with
resistance and susceptibility to disease caused by the pathogen Alternaria alternata at
UFRGS and with a short 1
st internode length. The growth habit and length of the first
internode of six alfalfa genotypes were evaluated, with this character considered as a
complementary morphological marker to select alfalfa populations more adapted to
grazing. Decumbent habit and shorter internode lengths were considered positive
because are indicative of adaptation to grazing. Several alfalfa genotypes were selected:
Crioula resistant (CR) and susceptible (CR) to Alternaria alternata, original seeds (CO),
E1C4 (Crioula 1st internode short; 4th cycle of selection, realized by UFRGS) resistant
(ECR) and susceptible (ECS) to Alternaria alternata, and E1C4 original seeds (ECO).
The plants and treatments were arranged at random (n= 20), and every seedling was
considered as one replicate of each genotype. The selection for plant resistance to
grazing tolerance was carried out from March 2015 to April 2015 in a growth chamber
at the Institute of Agrobiotechnology (IdAB) in Spain. To evaluate the length of the 1st
internode, the seeds were sterilized, mechanically scarified with sand paper to break
dormancy and then placed on water-agar plates in a growth chamber at 25 °C until the
radicles of the seedlings were about 10 mm long. Then, seedlings were transferred into a
glass jars containing nutritive solution with nitrate as nitrogen source. On seventh day,
the growth habit of the seedlings were evaluated for erect or decumbent growth habit
and after that, the seedlings were transferred to plates and pictures were taken daily
during 7 days to measure the size of the internodes with the software Image J. Data
were analyzed using Tukey's test (SAS Inst. Inc., Cary, NC). The CR and ECO alfalfa
genotypes showed the best results (P<0.005) for both variables evaluated demonstrating
habit tending to decumbent and length of the 1st internode 0.429 and 0.463 cm
respectively, at the last day of evaluation (day 15). These genotypes will be used for
subsequent research and selection cycles in the UFRGS Experimental Station., This work was supported by the AGL2014-52396-P grants from the
Spanish Ministry of Science and Innovation., Peer reviewed