OPTIMIZACION DE LA EFICIENCIA EN EL USO DE AGUA Y DEL NITROGENO POR LA VID Y DE LA CALIDAD DE LA UVA Y EL VINO, COMBINANDO MATERIAL GENETICO Y FERTIRRIGACION SOSTENIBLE

AGL2017-83738-C3-1-R

Nombre agencia financiadora Agencia Estatal de Investigación
Acrónimo agencia financiadora AEI
Programa Programa Estatal de I+D+i Orientada a los Retos de la Sociedad
Subprograma Programa Estatal de I+D+i Orientada a los Retos de la Sociedad
Convocatoria Retos Investigación: Proyectos I+D+i
Año convocatoria 2017
Unidad de gestión Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016
Centro beneficiario UNIVERSIDAD DE LAS ISLAS BALEARES
Identificador persistente http://dx.doi.org/10.13039/501100011033

Publicaciones

Found(s) 4 result(s)
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The intra-cultivar variability on water use efficiency at different water status as a target selection in grapevine: Influence of ambient and genotype

Digital.CSIC. Repositorio Institucional del CSIC
  • Tortosa, Ignacio
  • Escalona, José Mariano
  • Douthe, Cyril
  • Pou, Alicia
  • García-Escudero, E.
  • Toro, Guillermo
  • Medrano, Hipólito
To face the challenges induces by the climate change a better water use in agriculture is needed. One of the ways to get it is the genetic selection and breeding programs of genotypes focused on their water use efficiency (WUE). Grapevine crop is commonly growing under water stress conditions; to improve their WUE is a general goal for viticulture. In this study, we show the variability in WUE among clones of Tempranillo, cvar, grown under both pot and field conditions, all submitted to a large range of water availability, and along three consecutive years. Leaf net photosynthesis rate (A), stomatal conductance (g) were measured, and intrinsic water use efficiency (WUE) was computed as the ratio A/g. Firstly, we observed that the WUE showed important variations among clones. Field-growing plants consistently showed higher WUE than pot growing ones, and an important year effect was observed. The differences among genotypes were significant in pot conditions, but not in field. Nevertheless, the present results show intra-cultivar variability in Tempranillo in WUE, and therefore the possibility to build a selection program based in this criterion., This work was performed with the financial support from the Ministerio de Economía y Competitividad (MINECO, Spain) (project AGL2014-54201-C4-1-R and AGL2017-83738-C3-1-R) and a pre-doctoral fellowship BES-2015-073331). The authors would like to thank Mr. Miquel Truyols and collaborators of the UIB Experimental Field (UIB Grant 15/2015) for their support to our experiments.




Variability in water use efficiency of grapevine tempranillo clones and stability over years at field conditions

Digital.CSIC. Repositorio Institucional del CSIC
  • Tortosa, Ignacio
  • Douthe, Cyril
  • Pou, Alicia
  • Balda, Pedro
  • Hernández-Montes, E.
  • Toro, Guillermo
  • Escalona, José Mariano
  • Medrano, Hipólito
One way to face the consequences of climate change and the expected increase in water availability in agriculture is to find genotypes that can sustain production at a lower water cost. This theoretically can be achieved by using genetic material with an increased water use efficiency. We compared the leaf Water Use Efficiency (WUEi) under realistic field conditions in 14 vine genotypes of the Tempranillo cultivar (clones), in two sites of Northern Spain for three and five years each to evaluate (1) if a clonal diversity exists for this traits among those selected clones and (2) the stability of those differences over several years. The ranking of the different clones showed significant differences in WUE that were maintained over years in most of the cases. Different statistical analyses gave coincident information and allowed the identification of some clones systematically that had a higher WUE or a lower WUE. These methods also allowed the identification of the underlying physiological process that caused those differences and showed that clones with a higher WUE are likely to have an increased photosynthetic capacity (rather than a different stomatal control). Those differences could be useful to orientate the decision for vines selection programs in the near future., This work was performed with the financial support from the Spanish Ministry of Science and Technology (project AGL2014-54201-C4-1-R and AGL2017-83738-C3-1-R; Agencia Española de Investigación
AEI; Fondos FEDER) and a pre-doctoral fellowship BES-2015-073331) with a narrow collaboration inside the Associated Unit ICVV-INAGEA.UIB.




Comparing Selection Criteria to Select Grapevine Clones by Water Use Efficiency

Digital.CSIC. Repositorio Institucional del CSIC
  • Mairata, Andreu
  • Tortosa, Ignacio
  • Douthe, Cyril
  • Escalona, José Mariano
The current climate change is forcing growth-adapted genotypes with a higher water use efficiency (WUE). However, the evaluation of WUE is being made by different direct and indirect parameters such as the instantaneous leaf WUE (WUE) and isotopic discrimination of carbon (δC) content of fruits. In the present work, WUE has been evaluated in these two ways in a wide collection of grapevine genotypes, including Tempranillo and Garnacha clones, and Tempranillo on different rootstocks (T-rootstocks). A total of 70 genotypes have been analysed in four experimental fields over two years. The parameters used to measure WUE were the bunch biomass isotopic discrimination (δC) and the intrinsic WUE (WUE), defined as the ratio between net CO assimilation and stomatal conductance. The genotypes with the highest and lowest WUE were identified, differences between them being found to be of more than 10%. Generally, the two parameters showed coincidences in the clones with the highest and lowest WUE, suggesting that both are valuable tools to classify genotypes by their WUE in grapevine breeding programs. However, δC seemed to be a better indicator for determining WUE because it represents the integration over the synthesis time of the sample analysed (mainly sugars from ripening grapes), which coincides with the driest period for the crop. Moreover, the WUE is a variable parameter in the plant and it is more dependent on the environmental conditions. The present work suggests that carbon isotopic discrimination could be an interesting parameter for the clonal selection criteria in grapevines by WUE. The main reasons were its better discrimination between clones, the fact that sampling is less time-consuming and easier to do than WUE, and that the samples can be stored for late determinations, increasing the number of samples that can be analysed., This work was carried out with financial support from the Spanish Ministry of Science
and Technology (FEDER/Ministerio de Ciencia, Innovación y Universidades–Agencia Estatal de
Investigación/_AGL2017-83738-C3-1-R) and a pre-doctoral fellowship (PRE2019-089110) with a
narrow collaboration inside the Associated Unit ICVV-INAGEA.UIB.




Ecophysiological responses of grapevine rootstocks to water deficit

Digital.CSIC. Repositorio Institucional del CSIC
  • Pérez-Álvarez, Eva Pilar
  • Intrigliolo, Diego S.
  • Martínez-Moreno, Alejandro
  • García-Sánchez, Francisco
  • Parra Gómez, Margarita
  • Alfosea-Simón, Marina
  • Buesa, Ignacio
The use of rootstocks tolerant to soil water deficit is an interesting strategy to face the challenges posed by limited water availability. Currently, several nurseries are breeding new genotypes aiming to improve the water stress tolerance of grapevine, but the physiological basis of its responses under water stress are largely unknown. For this purpose, an ecophysiological assessment of the conventional 110-Rich-ter (110R) and SO4, and the new M1 and M4 rootstocks was carried out in ungrafted potted plants. During one season, these Vitis genotypes were grown under greenhouse conditions and subjected to two water regimes, well-watered (WW) and deficit irrigation (DI). Water potentials of plants under DI down to <-1.4 MPa, and net photosynthesis (A) <5 μmol COms did not cause leaf oxidative stress damage compared to WW conditions in all genotypes. The antioxidant capacity was sufficient to neutralize the mild oxidative stress suffered. Under both water regimes, gravimetric differences in daily water use were observed among genotypes, leading to differences in the biomass of roots and shoots. Under WW conditions, SO4 and 110R were the most vigorous and M1 and M4 the least. However, under DI, SO4 exhibited the greatest reduction in biomass, while 110R showed the lowest. Remarkably, under these conditions, SO4 reached the least negative stem water potential and showed the highest hydraulic conductance values. Conversely, M1 reduced the most stomatal conductance, transpiration and A. Overall, 110R achieved the highest biomass water use efficiency in response to DI, and SO4 the lowest, while M-rootstocks showed intermediate values. Our results suggest that there are differences in water use regulation among genotypes attributed not only to differences in stomatal regulation but also to plant hydraulic conductance. Therefore, it is hypothesized that differences in genotype performance may be due to root anatomical-morphological differences and to several physiological processes such as growth inhibition, osmotic adjustment, antioxidant production, nutrient translocation capacity, etc. Further studies are needed to confirm these differential ecophysiological responses of Vitis species under water stress, particularly under field and grafted conditions., This work received the financial support from the AEI with
FEDER co-funding (WANUGRAPE (AGL2017-83738-C3-1R)
and WANUGRAPE4.0 (PDC2021-121210-C21). E.P. Pérez-Álvarez
and I. Buesa acknowledges the postdoctoral financial
support received from “Juan de la Cierva” Spanish Program
(IJC2019-040502-I and FJC2019-042122-I, respectively).
Thanks are also due to J.S. Rubio, V. Graffato and A. Castellana
for their help in the greenhouse works.