BUSCADOR RECOLECTA

Introduction: cycling Dof transcription factors (CDFs) have been involved in different aspects of plant growth and development. In Arabidopsis and tomato, one member of this family (CDF1) has recently been associated with the regulation of primary metabolism and abiotic stress responses, but their roles in crop production under open field conditions remain unknown. Methods: in this study, we compared the growth, and tuber yield and composition of plants ectopically expressing the CDF1 gene from Arabidopsis under the control of the 35S promoter with wild-type (WT) potato plants cultured in growth chamber and open field conditions. Results: in growth chambers, the 35S::AtCDF1 plants showed a greater tuber yield than the WT by increasing the biomass partition for tuber development. Under field conditions, the ectopic expression of CDF1 also promoted the sink strength of the tubers, since 35S::AtCDF1 plants exhibited significant increases in tuber size and weight resulting in higher tuber yield. A metabolomic analysis revealed that tubers of 35S::AtCDF1 plants cultured under open field conditions accumulated higher levels of glucose, starch and amino acids than WT tubers. A comparative proteomic analysis of tubers of 35S::AtCDF1 and WT plants cultured under open field conditions revealed that these changes can be accounted for changes in the expression of proteins involved in energy production and different aspects of C and N metabolism. Discussion: The results from this study advance our collective understanding of the role of CDFs and are of great interest for the purposes of improving the yield and breeding of crop plants., This study was supported by the Ministerio de Ciencia e Innovación (MCIN) and the Agencia Estatal de Investigación (AEI) / 10.13039/501100011033/ (grants RTA2015-00014-c02-00 to JM and SGN; PID2020-114165RR-C21 to JM), (BIO2017-82873-R to JVC) (PID2019-104685GB-100 to JP-R, EB-F and FJM), the Gobierno de Navarra (PC036-037 BIOMEF to EB-F and FJM) and the Vicerrectorado de Investigación de la Universitat Politècnica de València (PAID-11-21 to SGN), J.C is funded by the National Agency for Research and Development (ANID) Chile with Program FONDECYT Regular 1190812 and ANID-Millennium Science Initiative Program-ICN17_022" and The GEP Chinese pre-doctoral fellowship to LY. We also want to acknowledge the "Severo Ochoa Program for Centres of Excellence in R&D" (CEX2020-000999-S) supported by MCIN/AEI/10.13039/501100011033 for supporting the scientific services used in this study.

[EN]
Nitrate is an essential macronutrient and a signal molecule that regulates the expression of multiple genes involved in plant growth and development. Here, we describe the participation of Arabidopsis DNA binding with one finger (DOF) transcription factor CDF3 in nitrate responses and shows that CDF3 gene is induced under nitrate starvation. Moreover, knockout cdf3 mutant plants exhibit nitrate-dependent lateral and primary root modifications, whereas CDF3 overexpression plants show increased biomass and enhanced root development under both nitrogen poor and rich conditions. Expression analyses of 35S::CDF3 lines reveled that CDF3 regulates the expression of an important set of nitrate responsive genes including, glutamine synthetase-1, glutamate synthase-2, nitrate reductase-1, and nitrate transporters NRT2.1, NRT2.4, and NRT2.5 as well as carbon assimilation genes like PK1 and PEPC1 in response to N availability. Consistently, metabolite profiling disclosed that the total amount of key N metabolites like glutamate, glutamine, and asparagine were higher in CDF3-overexpressing plants, but lower in cdf3-1 in N limiting conditions. Moreover, overexpression of CDF3 in tomato increased N accumulation and yield efficiency under both optimum and limiting N supply. These results highlight CDF3 as an important regulatory factor for the nitrate response, and its potential for improving N use efficiency in crops, This work was supported by grants from the National Institute for Agriculture and Food Research and Technology (INIA; RTA2015-00014-c02-01 to JM) as well as from the National Commission for Scientific and Technological Research (CONICYT; REDI170024 to JM, JC), ANID - Millennium Science Initiative Program - ICN17_022 to JC, Fondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT 1190812 and 1170926 to JC), Basque and Spanish Government (IT-932-16 and BIO2017-84035-R to DM), UE Prima (PCI2019-103610 to JM) MINECO-Spain (BIO2017-82873-R to JV-C) and INIA pre-doctoral fellowship to JD-F. We also want to acknowledge the "Severo Ochoa Program for Centers of Excellence in R&D" from the Agencia Estatal de Investigacion of Spain (grant SEV-2016-0672) for supporting the scientific services used in this work.

[EN] IntroductionCycling Dof transcription factors (CDFs) have been involved in different aspects of plant growth and development. In Arabidopsis and tomato, one member of this family (CDF1) has recently been associated with the regulation of primary metabolism and abiotic stress responses, but their roles in crop production under open field conditions remain unknown. MethodsIn this study, we compared the growth, and tuber yield and composition of plants ectopically expressing the CDF1 gene from Arabidopsis under the control of the 35S promoter with wild-type (WT) potato plants cultured in growth chamber and open field conditions. ResultsIn growth chambers, the 35S::AtCDF1 plants showed a greater tuber yield than the WT by increasing the biomass partition for tuber development. Under field conditions, the ectopic expression of CDF1 also promoted the sink strength of the tubers, since 35S::AtCDF1 plants exhibited significant increases in tuber size and weight resulting in higher tuber yield. A metabolomic analysis revealed that tubers of 35S::AtCDF1 plants cultured under open field conditions accumulated higher levels of glucose, starch and amino acids than WT tubers. A comparative proteomic analysis of tubers of 35S::AtCDF1 and WT plants cultured under open field conditions revealed that these changes can be accounted for changes in the expression of proteins involved in energy production and different aspects of C and N metabolism. DiscussionThe results from this study advance our collective understanding of the role of CDFs and are of great interest for the purposes of improving the yield and breeding of crop plants., We thank Manuel Sanchez-Perales and Antonio Ramos for his technical assistance and Mike Bennet for English edditing. This study was supported by the Ministerio de Ciencia e Innovacion (MCIN) and the Agencia Estatal de Investigacion (AEI)/10.13039/501100011033/(grants RTA2015-00014-c02-00 to JM and SGN; PID2020-114165RR-C21 to JM), (BIO2017-82873-R to JVC) (PID2019-104685GB-100 to JP-R, EB-F and FJM), the Gobierno de Navarra (PC036-037 BIOMEF to EB-F and FJM ) and the Vicerrectorado de Investigacion de la Universitat Politecnica de Valencia (PAID-11-21 to SGN), J.C is funded by the National Agency for Research and Development (ANID) Chile with Program FONDECYT Regular 1190812 and ANID-Millennium Science Initiative Program-ICN17_022"and The GEP Chinese pre-doctoral fellowship to LY. We also want to acknowledge the "Severo Ochoa Program for Centres of Excellence in R & D"(CEX2020-000999-S) supported by MCIN/AEI/10.13039/501100011033 for supporting the scientific services used in this study .

[EN] In terrestrial environments, water and nutrient availabilities and temperature conditions are highly variable, and especially in extreme environments limit survival, growth, and reproduction of plants. To sustain growth and maintain cell integrity under unfavourable environmental conditions, plants have developed a variety of biochemical and physiological mechanisms, orchestrated by a large set of stress-responsive genes and a complex network of transcription factors. Recently, cycling DOF factors (CDFs), a group of plant-specific transcription factors (TFs), were identified as components of the transcriptional regulatory networks involved in the control of abiotic stress responses. The majority of the members of this TF family are activated in response to a wide range of adverse environmental conditions in different plant species. CDFs regulate different aspects of plant growth and development such as photoperiodic flowering-time control and root and shoot growth. While most of the functional characterization of CDFs has been reported in Arabidopsis, recent data suggest that their diverse roles extend to other plant species. In this review, we integrate information related to structure and functions of CDFs in plants, with special emphasis on their role in plant responses to adverse environmental conditions., This work was supported by grants from The National Institute for Agriculture and Food Research and Technology (INIA) (RTA201500014-c02-01) to JM as well as from the National Commission for Scientific and Technological Research (CONICYT) (REDI170024 to JM) UE Prima (PCI2019-103610 to JM) and from MINECO (BIO2017-82873 to JVC). We also want to acknowledge the `Severo Ochoa Program for Centres of Excellence in R&D' from the Agencia Estatal de Investigacion of Spain (Grant SEV-2016-0672; 2017-2021) for supporting the scientific services used in this work.

Over the last three decades, novel "omics" platform technologies for the sequencing of DNA and complementary DNA (cDNA) (RNA-Seq), as well as for the analysis of proteins and metabolites by mass spectrometry, have become more and more available and increasingly found their way into general laboratory life. With this, the ability to generate highly multivariate datasets on the biological systems of choice has increased tremendously. However, the processing and, perhaps even more importantly, the integration of "omics" datasets still remains a bottleneck, although considerable computational and algorithmic advances have been made in recent years. In this mini-review, we use a number of recent "multi-omics" approaches realized in our laboratories as a common theme to discuss possible pitfalls of applying "omics" approaches and to highlight some useful tools for data integration and visualization in the form of an exemplified case study. In the selected example, we used a combination of transcriptomics and metabolomics alongside phenotypic analyses to functionally characterize a small number of Cycling Dof Transcription Factors (CDFs). It has to be remarked that, even though this approach is broadly used, the given workflow is only one of plenty possible ways to characterize target proteins.

Se realiza un estudio pormenorizado de la expresión y localización tisular del gen que codifica la enzima Asparagina Sintetasa 1 (HvASN1) durante el desarrollo y la germinación de las semillas de cebada, así como de sus posible reguladores transcripcionales HvbZIP53 y HvBLZ1.

Eukaryotic cells under certain physiological and environmental conditions produce a massive accumulation of proteins in the endoplasmic reticulum, leading to teh "endoplasmatic reticulum stress".

Se describe la regulación transcripcional del gen que codifica la enzima Asparagina Sintetasa 1 (HvASN1) durante el desarrollo y la germinación de las semillas de cebada. Los resultados presentados indican que la interacción proteína-proteína HvbZIP53-HvBLZ1 activa transcripcionalmente la expresión de HvASN1 durante la germinación de las semillas de cebada.

Mediante una estrategia que combina análisis transcriptómicos globales en bases de datos públicas y el cribado de una genoteca en levaduras de aprox. 1200 ORFs de factores transcripcionales de Arabidopsis thaliana, se identifican nuevos componentes de la respuesta a la "proteína mal plegada" del Retículo Endoplásmico frente a distintos estreses abióticos.

The barley endo-b-mannanase (MAN) gene family (HvMAN1-6) has been identified and the expression of its members analyzed throughout different plant organs, and upon grain development and germination. The HvMAN1 gene has been found to be highly expressed in developing and germinating grains. The MAN (EC 3.2.1.78) enzymatic activity gets a maximum in grains at 48 h of germination (post-germination event). Immunolocalization of mannan polymers in grains has revealed the presence of these polysaccharides in the endosperm cell walls (CWs). By mRNA in situ hybridization assays, the HvMAN1 transcripts have been localized to the aleurone layer, but not to the dead starchy endosperm cells. These data suggest that MAN1 is synthesized in the aleurone layer during early grain imbibition and moves potentially through the apoplast to the endosperm where the hydrolysis of the mannan polymers takes place after germination sensu stricto. Hence, mannans in the starchy endosperm CWs, besides their structural function, could be used as reserve compounds upon barley post-germination.