ESTUDIO MULTIDISCIPLINAR DIRIGIDO A LA MEJORA EN LA EFICIENCIA EN EL USO DE LOS RECURSOS EN CEREALES EXPUESTOS A UN MEDIO AMBIENTE CAMBIANTE

PCIN-2017-007

Nombre agencia financiadora Agencia Estatal de Investigación
Acrónimo agencia financiadora AEI
Programa Programa Estatal de I+D+i Orientada a los Retos de la Sociedad
Subprograma Programación Conjunta Internacional
Convocatoria Proyectos I+D
Año convocatoria 2017
Unidad de gestión Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016
Centro beneficiario AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC)
Identificador persistente http://dx.doi.org/10.13039/501100011033

Publicaciones

Found(s) 9 result(s)
Found(s) 1 page(s)

Assessing the evolution of wheat grain traits during the last 166 years using archived samples

Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
  • Mariem, S.B.
  • Gámez Guzmán, Angie Lorena
  • Larraya Reta, Luis María
  • Fuertes Mendizabal, Teresa
  • Cañameras, Nuria
  • Araus, José Luis
  • Aranjuelo Michelena, Iker
  • McGrath, Steve P.
  • Hawkesford, Malcolm J.
  • González Murua, Carmen
  • Gaudeul, Myriam
  • Medina, Leopoldo
  • Paton, Alan
  • Cattivelli, Luigi
  • Fangmeier, Andreas
  • Bunce, James
  • Tausz-Posch, Sabine
  • Macdonald, Andy J.
Incluye material complementario, The current study focuses on yield and nutritional quality changes of wheat grain over the last 166 years. It is based on wheat grain quality analyses carried out on samples collected between 1850 and 2016. Samples were obtained from the Broadbalk Continuous Wheat Experiment (UK) and from herbaria from 16 different countries around the world. Our study showed that, together with an increase in carbohydrate content, an impoverishment of mineral composition and protein content occurred. The imbalance in carbohydrate/protein content was specially marked after the 1960’s, coinciding with strong increases in ambient [CO2] and temperature and the introduction of progressively shorter straw varieties. The implications of altered crop physiology are discussed., This work was supported by the Spanish Innovation and Universities Ministry (AGL2016-79868-R; PCIN-2017-007), BBSRC Institute Strategic Programme grants, Designing Future Wheat (BB/P016855/1) and Soil to Nutrition (S2N, BBS/E/C/00I0310), the Long-term Experiments National Capability grant (BBS/E/C/000J0300), the Lawes Agricultural Trust and the Basque Country Government consolidated group programme (IT‐932‐16).




Differential Flag Leaf and Ear Photosynthetic Performance Under Elevated (CO2) Conditions During Grain Filling Period in Durum Wheat

Dadun. Depósito Académico Digital de la Universidad de Navarra
  • Gámez, A.L. (Angie L.)
  • Vicente, R. (Rubén)
  • Sanchez-Bragado, R. (Rut)
  • Jauregui, I. (Iván)
  • Morcuende, R. (Rosa)
  • Goicoechea, N. (Nieves)
  • Aranjuelo, I. (Iker)
Elevated concentrations of CO2 (CO2) in plants with C3 photosynthesis metabolism,
such as wheat, stimulate photosynthetic rates. However, photosynthesis tends to
decrease as a function of exposure to high (CO2) due to down-regulation of
the photosynthetic machinery, and this phenomenon is defined as photosynthetic
acclimation. Considerable efforts are currently done to determine the effect of
photosynthetic tissues, such us spike, in grain filling. There is good evidence that the
contribution of ears to grain filling may be important not only under good agronomic
conditions but also under high (CO2). The main objective of this study was to compare
photoassimilate production and energy metabolism between flag leaves and glumes as
part of ears of wheat (Triticum turgidum L. subsp. durum cv. Amilcar) plants exposed to
ambient [a(CO2)] and elevated [e(CO2)] (CO2) (400 and 700 µmol mol−1
, respectively).
Elevated CO2 had a differential effect on the responses of flag leaves and ears. The ears
showed higher gross photosynthesis and respiration rates compared to the flag leaves.
The higher ear carbohydrate content and respiration rates contribute to increase the
grain dry mass. Our results support the concept that acclimation of photosynthesis to
e(CO2) is driven by sugar accumulation, reduction in N concentrations and repression
of genes related to photosynthesis, glycolysis and the tricarboxylic acid cycle, and
that these were more marked in glumes than leaves. Further, important differences
are described on responsiveness of flag leaves and ears to e(CO2) on genes linked
with carbon and nitrogen metabolism. These findings provide information about the
impact of e(CO2) on ear development during the grain filling stage and are significant for
understanding the effects of increasing (CO2) on crop yield.




Photosynthetic metabolism under stressful growth conditions as a bases for crop breeding and yield improvement

Digital.CSIC. Repositorio Institucional del CSIC
  • Morales, Fermín
  • Ancín, María
  • Fakhet, Dorra
  • González-Torralba, Jon
  • Gámez, Angie L.
  • Seminario, Amaia
  • Soba, David
  • Ben Mariem, Sinda
  • Garriga, Miguel
  • Aranjuelo, Iker
23 Pags.- 4 Figs. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access
article distributed under the terms and conditions of the Creative Commons Attribution
(CC BY) license., Increased periods of water shortage and higher temperatures, together with a reduction in
nutrient availability, have been proposed as major factors that negatively impact plant development.
Photosynthetic CO2 assimilation is the basis of crop production for animal and human food, and
for this reason, it has been selected as a primary target for crop phenotyping/breeding studies.
Within this context, knowledge of the mechanisms involved in the response and acclimation of
photosynthetic CO2 assimilation to multiple changing environmental conditions (including nutrients,
water availability, and rising temperature) is a matter of great concern for the understanding of plant
behavior under stress conditions, and for the development of new strategies and tools for enhancing
plant growth in the future. The current review aims to analyze, from a multi-perspective approach
(ranging across breeding, gas exchange, genomics, etc.) the impact of changing environmental
conditions on the performance of the photosynthetic apparatus and, consequently, plant growth., This work was supported by the Spanish Innovation and Universities Ministry (AGL2016-79868-R; PCIN-2017-007)., Peer reviewed




Differential Flag Leaf and Ear Photosynthetic Performance Under Elevated (CO2) Conditions During Grain Filling Period in Durum Wheat

Digital.CSIC. Repositorio Institucional del CSIC
  • Gámez, Angie L.
  • Vicente Pérez, Rubén
  • Sanchez-Bragado, Rut
  • Jauregui, Iván
  • Morcuende, Rosa
  • Goicoechea, Nieves
  • Aranjuelo, Iker
12 páginas, 4 figuras, Elevated concentrations of CO2 (CO2) in plants with C3 photosynthesis metabolism, such as wheat, stimulate photosynthetic rates. However, photosynthesis tends to decrease as a function of exposure to high (CO2) due to down-regulation of the photosynthetic machinery, and this phenomenon is defined as photosynthetic
acclimation. Considerable efforts are currently done to determine the effect of photosynthetic tissues, such us spike, in grain filling. There is good evidence that the contribution of ears to grain filling may be important not only under good agronomic conditions but also under high (CO2). The main objective of this study was to compare photoassimilate production and energy metabolism between flag leaves and glumes as part of ears of wheat (Triticum turgidum L. subsp. durum cv. Amilcar) plants exposed to ambient [a(CO2)] and elevated [e(CO2)] (CO2) (400 and 700 µmol mol−1, respectively). Elevated CO2 had a differential effect on the responses of flag leaves and ears. The ears showed higher gross photosynthesis and respiration rates compared to the flag leaves. The higher ear carbohydrate content and respiration rates contribute to increase the grain dry mass. Our results support the concept that acclimation of photosynthesis to e(CO2) is driven by sugar accumulation, reduction in N concentrations and repression of genes related to photosynthesis, glycolysis and the tricarboxylic acid cycle, and that these were more marked in glumes than leaves. Further, important differences are described on responsiveness of flag leaves and ears to e(CO2) on genes linked with carbon and nitrogen metabolism. These findings provide information about the impact of e(CO2) on ear development during the grain filling stage and are significant for understanding the effects of increasing (CO2) on crop yield, This research was supported by the Spanish Innovation and Universities Ministry (PCIN-2017-007) and the Ministry of Education and Sciences (grants PID2019-107154RB100; PID2019-110445RB-100). RV was the recipient of an FPI fellowship from the Spanish Ministry of Economy and Competitiveness (BES-2010-031029). RS-B was supported by the Juan de la Cierva program JDC-Formación (FJCI-2016-28164)., Peer reviewed




Short-Term Exposure to High Atmospheric Vapor Pressure Deficit (VPD) Severely Impacts Durum Wheat Carbon and Nitrogen Metabolism in the Absence of Edaphic Water Stress

Digital.CSIC. Repositorio Institucional del CSIC
  • Fakhet, Dorra
  • Morales, Fermín
  • Jauregui, Iván
  • Erice, Gorka
  • Aparicio-Tejo, Pedro María
  • González-Murua, Carmen
  • Aroca, Ricardo
  • Irigoyen, Juan José
  • Aranjuelo, Iker
Low atmospheric relative humidity (RH) accompanied by elevated air temperature and decreased precipitation are environmental challenges that wheat production will face in future decades. These changes to the atmosphere are causing increases in air vapor pressure deficit (VPD) and low soil water availability during certain periods of the wheat-growing season. The main objective of this study was to analyze the physiological, metabolic, and transcriptional response of carbon (C) and nitrogen (N) metabolism of wheat (<i>Triticum durum</i> cv. Sula) to increases in VPD and soil water stress conditions, either alone or in combination. Plants were first grown in well-watered conditions and near-ambient temperature and RH in temperature-gradient greenhouses until anthesis, and they were then subjected to two different water regimes well-watered (WW) and water-stressed (WS), i.e., watered at 50% of the control for one week, followed by two VPD levels (low, 1.01/0.36 KPa and high, 2.27/0.62 KPa; day/night) for five additional days. Both VPD and soil water content had an important impact on water status and the plant physiological apparatus. While high VPD and water stress-induced stomatal closure affected photosynthetic rates, in the case of plants watered at 50%, high VPD also caused a direct impairment of the RuBisCO large subunit, RuBisCO activase and the electron transport rate. Regarding N metabolism, the gene expression, nitrite reductase (NIR) and transport levels detected in young leaves, as well as determinations of the δ<sup>15</sup>N and amino acid profiles (arginine, leucine, tryptophan, aspartic acid, and serine) indicated activation of N metabolism and final transport of nitrate to leaves and photosynthesizing cells. On the other hand, under low VPD conditions, a positive effect was only observed on gene expression related to the final step of nitrate supply to photosynthesizing cells, whereas the amount of <sup>15</sup>N supplied to the roots that reached the leaves decreased. Such an effect would suggest an impaired N remobilization from other organs to young leaves under water stress conditions and low VPD., This work was supported by the Spanish Innovation and Universities Ministry (AGL2016-79868-R; 427 PCIN-2017-007) and the Basque Country Government consolidated group program (IT-932-16)., Peer reviewed




Photosynthesis in a Changing Global Climate: Scaling Up and Scaling Down in Crops

Digital.CSIC. Repositorio Institucional del CSIC
  • Baslam, Marouane
  • Mitsui, Toshiaki
  • Hodges, Michael
  • Priesack, Eckart
  • Herritt, Matthew T.
  • Aranjuelo, Iker
  • Sanz-Sáez, Álvaro
Photosynthesis is the major process leading to primary production in the Biosphere. There is a total of 7000bn tons of CO in the atmosphere and photosynthesis fixes more than 100bn tons annually. The CO assimilated by the photosynthetic apparatus is the basis of crop production and, therefore, of animal and human food. This has led to a renewed interest in photosynthesis as a target to increase plant production and there is now increasing evidence showing that the strategy of improving photosynthetic traits can increase plant yield. However, photosynthesis and the photosynthetic apparatus are both conditioned by environmental variables such as water availability, temperature, [CO], salinity, and ozone. The “omics” revolution has allowed a better understanding of the genetic mechanisms regulating stress responses including the identification of genes and proteins involved in the regulation, acclimation, and adaptation of processes that impact photosynthesis. The development of novel non-destructive high-throughput phenotyping techniques has been important to monitor crop photosynthetic responses to changing environmental conditions. This wealth of data is being incorporated into new modeling algorithms to predict plant growth and development under specific environmental constraints. This review gives a multi-perspective description of the impact of changing environmental conditions on photosynthetic performance and consequently plant growth by briefly highlighting how major technological advances including omics, high-throughput photosynthetic measurements, metabolic engineering, and whole plant photosynthetic modeling have helped to improve our understanding of how the photosynthetic machinery can be modified by different abiotic stresses and thus impact crop production., This work was supported by IRUEC project funded by EIG CONCERT-Japan 3rd Joint Call on “Food Crops and Biomass Production Technologies” under the Strategic International Research Cooperative Program of the Japan Science and Technology Agency (JST) and the Spanish Innovation and Universities Ministry (Acciones de programación conjunta Internacional, PCIN-2017-007), and by the ANR-14-CE19-0015 grant REGUL3P. A Grant for Promotion of KAAB Projects (Niigata University) from the Ministry of Education, Culture, Sports, Science, and Technology-Japan is also acknowledged.




Assessing the evolution of wheat grain traits during the last 166 years using archived samples

Digital.CSIC. Repositorio Institucional del CSIC
  • Ben Mariem, Sinda
  • Gámez, Angie L.
  • Larraya, Luis
  • Fuertes-Mendizabal, Teresa
  • Cañameras, Nuria
  • Araus, José Luis
  • McGrath, Steve P.
  • Hawkesford, Malcolm J.
  • González-Murua, Carmen
  • Gaudeul, Myriam
  • Medina, Leopoldo
  • Paton, Alan
  • Cattivelli, Luigi
  • Fangmeier, Andreas
  • Bunce, James
  • Tausz-Posch, Sabine
  • Macdonald, Andy J.
  • Aranjuelo, Iker
The current study focuses on yield and nutritional quality changes of wheat grain over the last 166 years. It is based on wheat grain quality analyses carried out on samples collected between 1850 and 2016. Samples were obtained from the Broadbalk Continuous Wheat Experiment (UK) and from herbaria from 16 different countries around the world. Our study showed that, together with an increase in carbohydrate content, an impoverishment of mineral composition and protein content occurred. The imbalance in carbohydrate/protein content was specially marked after the 1960’s, coinciding with strong increases in ambient [CO2] and temperature and the introduction of progressively shorter straw varieties. The implications of altered crop physiology are discussed., This work was supported by the Spanish Innovation and Universities Ministry (AGL2016-79868-R; PCIN-2017-007), BBSRC Institute Strategic Programme grants, Designing Future Wheat (BB/P016855/1) and Soil to Nutrition (S2N, BBS/E/C/00I0310), the Long-term Experiments National Capability grant (BBS/E/C/000J0300), the Lawes Agricultural Trust and the Basque Country Government consolidated group programme (IT‐932‐16)., Peer reviewed




Climate change, crop yields, and grain quality of c3 cereals: A meta‐analysis of [co2], temperature, and drought effects

Digital.CSIC. Repositorio Institucional del CSIC
  • Ben Mariem, Sinda
  • Soba, David
  • Zhou, Bangwei
  • Loladze, Irakli
  • Morales, Fermín
  • Aranjuelo, Iker
Cereal yield and grain quality may be impaired by environmental factors associated with climate change. Major factors, including elevated CO2 concentration ([CO2]), elevated temperature, and drought stress, have been identified as affecting C3 crop production and quality. A me-ta‐analysis of existing literature was performed to study the impact of these three environmental factors on the yield and nutritional traits of C3 cereals. Elevated [CO2] stimulates grain production (through larger grain numbers) and starch accumulation but negatively affects nutritional traits such as protein and mineral content. In contrast to [CO2], increased temperature and drought cause significant grain yield loss, with stronger effects observed from the latter. Elevated temperature decreases grain yield by decreasing the thousand grain weight (TGW). Nutritional quality is also negatively influenced by the changing climate, which will impact human health. Similar to drought, heat stress decreases starch content but increases grain protein and mineral concentra-tions. Despite the positive effect of elevated [CO2], increases to grain yield seem to be counterbal-anced by heat and drought stress. Regarding grain nutritional value and within the three environmental factors, the increase in [CO2] is possibly the more detrimental to face because it will affect cereal quality independently of the region., This work was supported by European Interest Group (EIG) CONCERT-Japan (IRUEC),
the Spanish Ministry of Science and Innovation (Spanish MINECO projects PCIN-2017-007 and
PID2019-110445RB-I00). Sinda Ben Mariem had a PhD grant from the Navarra Government




Functional traits of field-droughted contrasting rice genotypes reveal multiple independent genomic adaptations and metabolic responses

Digital.CSIC. Repositorio Institucional del CSIC
  • Baslam, Marouane
  • Takamatsu, Takeshi
  • Aycan, Murat
  • Fakhet, Dorra
  • Rezzouk, Fatima Zahra
  • Gakière, Bertrand
  • Araus, José Luis
  • Aranjuelo, Iker
  • Mitsui, Toshiaki
Drought is the most critical environmental stress limiting crop productivity and yield. Rice is one of the top leading food crops worldwide and modern rice varieties are notably drought-sensitive due to their high-water requirements. Generating drought-resilient varieties and fine-tuning their yield performance are time-consuming tasks that could be achieved by exploiting the genetic diversity expressed by the wild ancestors of current-day crop species. We conducted a large-scale field experiment, imposing pre-flowering and post-flowering water shortages on 20 Oryza genotypes. Using detailed agro-physiological, isotopomics, and metabolite characterization of different Asian and African rice accessions, we shed light on the evolutionary biases that have evolved into the spectrum of strategies used to adapt to drought and have added to the rice knowledge base. We find that relevant decreases in photosynthetic rates (linked with stomatal opening) that differed between genotypes. We also identified genotypic differences in core C and N metabolism, and central metabolic pathways, highlighting the possible mechanisms of drought tolerance. Additionally, data suggest strong genetic associations of several amino acids that are significantly enriched during drought. We provide strong evidence of the distinctiveness of agro-physiological and metabolic pathway behaviors to drought across the use of diverse germplasm resources. Also, the resulting data provide insight into the wide variety of germplasm responses triggered by drought., This work was supported by grants JPMJSC16C5 from EIG CONCERT–Japan under the Strategic International Collaborative Research Program by the JST SICORP and PCIN-2017-007 from the Spanish Science, by Innovation and Universities Ministry (Acciones de programación conjunta Internacional). A Grant for Promotion of KAAB Projects (Niigata University, Japan) from the Ministry of Education, Culture, Sports, Science, and Technology is also acknowledged.