MECANISMOS DE ADAPTACION A CONDICIONES NO OPTIMAS DE COBRE Y HIERRO EN LEVADURAS Y PLANTAS
PID2020-116940RB-I00
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Nombre agencia financiadora Agencia Estatal de Investigación
Acrónimo agencia financiadora AEI
Programa Programa Estatal de I+D+i Orientada a los Retos de la Sociedad
Subprograma Programa Estatal de I+D+i Orientada a los Retos de la Sociedad
Convocatoria Proyectos I+D
Año convocatoria 2020
Unidad de gestión Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020
Centro beneficiario AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC)
Identificador persistente http://dx.doi.org/10.13039/501100011033
Publicaciones
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The role of the Arabidopsis tandem zinc-finger C3H15 protein in metal homeostasis
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
- Andrés-Bordería, Amparo
- Mazuque-Pons, L.
- Romeu-Perales, M.
- Garcia-Molina, Antoni
- Martínez Pastor, María Teresa
- Sanz, Amparo
- Puig, Sergi
- Peñarrubia, L.
- Pérez-García, Ana
- Andrés Colás, Nuria
[EN] Living organisms have developed finely regulated homeostatic networks to mitigate the effects of environmental fluctuations in transition metal micronutrients, including iron, zinc, and copper. In Saccharomyces cerevisiae, , the tandem zinc-finger protein Cth2 post-transcriptionally regulates gene expression under conditions of iron deficiency by controlling the levels of mRNAs that code for non-essential ferroproteins. The molecular mechanism involves Cth2 binding to AU-rich elements present in the 3 ' ' untranslated region of target mRNAs, negatively affecting their stability and translation. Arabidopsis thaliana has two TZF proteins homologous to yeast Cth2, C3H14 and C3H15, which participate in cell wall remodelling. The present work examines the expression of representative metal homeostasis genes with putative AREs in plants with altered levels of C3H14 and C3H15 grown under varying metal availabilities. The results suggest that C3H15 may act as a post-transcriptional plant modulator of metal adequacy, as evidenced by the expression of SPL7, , the main transcriptional regulator under copper deficiency, and PETE2, , which encodes plastocyanin. In contrast to S. cerevisiae, , the plant C3H15 affects copper and zinc homeostasis rather than iron. When grown under copper-deficient conditions, adult C3H15OE OE plants exhibit lower chlorophyll content and photosynthetic efficiency compared to control plants, suggesting accelerated senescence. Likewise, metal content in C3H15OE(OE) plants under copper deficiency shows altered mobilization of copper and zinc to seeds. These data suggest that the C3H15 protein plays a role in modulating both cell wall remodelling and metal homeostasis. The interaction between these processes may be the cause of altered metal translocation., We acknowledge Dr Patricia Casino for her help with the overlapping of the TZF domains, Dr Zhou for the C3H14, C3H15 overexpressing seeds and c3h14c3h15 (+/-) +/-) mutant and Marta Olmos for her technical support. A. Sanz obtained a sabbatical year grant (UV-PDI_SAB-156039). We acknowledge the SCSIE (Universitat de Valencia) for the ICP-MS service. This research was funded by the Spanish Ministry of Economy and Competitiveness "PID2020-116940RB-I00 and PID2023-148124OB-I00 funded by MCIN/AEI/10.13039/501100011033" and CIGE/2021/092 funded by Generalitat Valenciana.