<p>In this study we develop a procedure for thermostable selection of random insertion mutants based on a gene cassette encoding a thermostable resistance to kanamycin flanked by the recognition sites (ME sites) for Tn5 transposase. The transposition library generated in vitro was initialy transformed in a PrimPol mutant HB27 derivative (ppol), which shows ~2 log fold transformation efficiency. This allowed the generation of the generation of a large library of insertion mutants (“Mother”), which was later transferred to a wild type HB27 strain (“Daughter”). Both libraries were sequenced straightforward and after several culture passages. See the manuscript for more details.</p>
<p>This dataset contains the raw data of the sequencing libraries as well as the GitHub repository containing the scrips and reports from the analysis.</p>

<p>Comparison of whole metagenome amplification competence by new piPolB-based MDA methods and commercial kits using a mini mock-metagenome containing high-GC sequences. </p>
<p>Mini mock metagenome made up of <i> E. coli, B. subtilis, P. aeruginosa and K. rhizophila genomes.</i> A total of 11 samples were analyzed:</p>
<ul>
<li>unamplified metagenome (NA and NA2)</li>
<li>samples obtained by random-primers MDA (RP-MDA and RP-MDA2)</li>
<li>PrimPol-MDA</li>
<li>new MDA with piPolB as only enzyme</li>
<li>piPolB with a previous DNA alkaline denaturation step (piPolB+D)</li>
<li>new MDA with piPolB and phi29 DNA polymerases (piMDA and piMDA2)</li>
<li>and the latter method with a previous denaturation step (piMDA+D and piMDA+D2)</li></ul>

<p>The authors have carried out a massive screening of pipolins in the bacterial genome database "Assembly" (NCBI). Then, they found integrases and new integration sites associated to pipolins. Pipolins and other MGEs (plasmids, ciMGEs, and phages) were annotated to characterize pipolin genetic content comparatively. Finally, they applied a recent method to detect recent genetic exchange events between pipolins and other MGEs based on the weighted gene repertoire relatedness and protein sequence identity.</p>

<p>This dataset contains several files created throughout the analysis. Files 1 and 2 are related to pipolin screening and plotting. Files 3 to 5 contain the list of genes classified as "recombining" or "non-recombining" encoded in the MGEs.</p>