CAMBIOS EN LA ESTRUCTURA 3D DE LA CROMATINA DURANTE EL ENVEJECIMIENTO
PID2022-141288NB-I00
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Nombre agencia financiadora Agencia Estatal de Investigación
Acrónimo agencia financiadora AEI
Programa Programa Estatal para Impulsar la Investigación Científico-Técnica y su Transferencia
Subprograma Subprograma Estatal de Generación de Conocimiento
Convocatoria Proyectos de I+D+I (Generación de Conocimiento y Retos Investigación)
Año convocatoria 2022
Unidad de gestión Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023
Centro beneficiario AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC)
Identificador persistente http://dx.doi.org/10.13039/501100011033
Publicaciones
Found(s) 2 result(s)
Found(s) 1 page(s)
Found(s) 1 page(s)
Ptychodera flava genomics and transcriptomics data
Digital.CSIC. Repositorio Institucional del CSIC
- Pérez-Posada, Alberto
- Lin, Che-Yi
- Fan, Tzu-Pei
- Lin, Ching-Yi
- Chen, Yi-Chih
- Gómez-Skarmeta, José Luis
- Yu, Jr-Kai
- Su, Yi-Hsien
- Tena, Juan J.
[Description of methods used for collection/generation of data] ATACseq and RNAseq methodology in different developmental stages, Analysis of the transcriptomes and chromatin accessibility of multiple developmental stages of the indirect-developing hemichordate Ptychodera flava. After the individual analysis of each sample, integration of ATACseq and RNAseq data was performed in order to reconstruct genetic and regulatory networks. These data were also compared to other species, what could shed light on deuterostome evolution., Ministerio de Ciencia e Innovación (grant PID2022-141288NB-I00)., supp_data_01.xlsx supp_data_02.xlsx supp_data_03.xlsx supp_data_04.xlsx supp_data_05.xlsx supp_data_06.xlsx supp_data_07.xlsx supp_data_08.xlsx supp_data_09.bed supp_data_10.tsv supp_data_11.tsv.gz supp_data_12.tsv.gz supp_data_13.xlsx supp_data_14.xlsx supp_data_15.xlsx supp_data_16.xlsx, Peer reviewed
An amphioxus neurula stage cell atlas supports a complex scenario for the emergence of vertebrate head mesoderm
Digital.CSIC. Repositorio Institucional del CSIC
- Grau-Bové, Xavier
- Subirana, Lucie
- Meister, Lydvina
- Soubigou, Anaël
- Neto, Ana
- Elek, Anamaria
- Naranjo, Silvia
- Fornas, Óscar
- Gómez-Skarmeta, José Luis
- Tena, Juan J.
- Irimia, Manuel
- Bertrand, Stephanie
- Sebé-Pedrós, Arnau
- Escrivá, Héctor
The emergence of new structures can often be linked to the evolution of novel
cell types that follows the rewiring of developmental gene regulatory subnetworks. Vertebrates are characterized by a complex body plan compared to
the other chordate clades and the question remains of whether and how the
emergence of vertebrate morphological innovations can be related to the
appearance of new embryonic cell populations. We previously proposed, by
studying mesoderm development in the cephalochordate amphioxus, a scenario for the evolution of the vertebrate head mesoderm. To further test this
scenario at the cell population level, we used scRNA-seq to construct a cell
atlas of the amphioxus neurula, stage at which the main mesodermal compartments are specified. Our data allowed us to validate the presence of a
prechordal-plate like territory in amphioxus. Additionally, the transcriptomic
profile of somite cell populations supports the homology between specific
territories of amphioxus somites and vertebrate cranial/pharyngeal and lateral
plate mesoderm. Finally, our work provides evidence that the appearance of
the specific mesodermal structures of the vertebrate head was associated to
both segregation of pre-existing cell populations, and co-option of new genes
for the control of myogenesis., The laboratory of H.E. and S.B. was supported by the CNRS, and by the “Agence Nationale de la Recheche” under the grants ANR-19-CE13-0011 to H.E. and ANR-21-CE13-0034 to S.B. Research in A.S-P. group was supported by the European Research Council (ERC-StG 851647 to A.S-P.) and the Spanish Ministry of Science and Innovation (PID2021-124757NB-I00 to A.S-P.). X.G-B. is supported by the European Union’s H2020 research and innovation program under Marie Sklodowska-Curie grant agreement 101031767 to X.G-B. A.E. was supported by FPI PhD fellowships from the Spanish Ministry of Science and Innovation. J.J.T. was supported by the Spanish Ministerio de Economía y Competitividad (grant PID2019-103921GB-I00 and PID2022-141288NB-I00 to J.T.). M.I. laboratory research has been funded by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (ERCCoG-LS2-101002275 to M.I.), by the Spanish Ministry of Economy and Competitiveness (PID2020-115040GB-I00 to M.I.) and by the ‘Centro de Excelencia Severo Ochoa 2013-2017’(SEV-2012-0208 to M. I.)., Peer reviewed
cell types that follows the rewiring of developmental gene regulatory subnetworks. Vertebrates are characterized by a complex body plan compared to
the other chordate clades and the question remains of whether and how the
emergence of vertebrate morphological innovations can be related to the
appearance of new embryonic cell populations. We previously proposed, by
studying mesoderm development in the cephalochordate amphioxus, a scenario for the evolution of the vertebrate head mesoderm. To further test this
scenario at the cell population level, we used scRNA-seq to construct a cell
atlas of the amphioxus neurula, stage at which the main mesodermal compartments are specified. Our data allowed us to validate the presence of a
prechordal-plate like territory in amphioxus. Additionally, the transcriptomic
profile of somite cell populations supports the homology between specific
territories of amphioxus somites and vertebrate cranial/pharyngeal and lateral
plate mesoderm. Finally, our work provides evidence that the appearance of
the specific mesodermal structures of the vertebrate head was associated to
both segregation of pre-existing cell populations, and co-option of new genes
for the control of myogenesis., The laboratory of H.E. and S.B. was supported by the CNRS, and by the “Agence Nationale de la Recheche” under the grants ANR-19-CE13-0011 to H.E. and ANR-21-CE13-0034 to S.B. Research in A.S-P. group was supported by the European Research Council (ERC-StG 851647 to A.S-P.) and the Spanish Ministry of Science and Innovation (PID2021-124757NB-I00 to A.S-P.). X.G-B. is supported by the European Union’s H2020 research and innovation program under Marie Sklodowska-Curie grant agreement 101031767 to X.G-B. A.E. was supported by FPI PhD fellowships from the Spanish Ministry of Science and Innovation. J.J.T. was supported by the Spanish Ministerio de Economía y Competitividad (grant PID2019-103921GB-I00 and PID2022-141288NB-I00 to J.T.). M.I. laboratory research has been funded by the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (ERCCoG-LS2-101002275 to M.I.), by the Spanish Ministry of Economy and Competitiveness (PID2020-115040GB-I00 to M.I.) and by the ‘Centro de Excelencia Severo Ochoa 2013-2017’(SEV-2012-0208 to M. I.)., Peer reviewed