Publicaciones

Found(s) 2 result(s)
Found(s) 1 page(s)

Dataset related to a study to identify genomic regions in susceptibility to Schistosoma mansoni infection in a murine backcross, Identification of genomic regions implicated in susceptibility to "Schistosoma mansoni" infection in a murine genetic model (backcross)

Digital.CSIC. Repositorio Institucional del CSIC
  • Hernández-Goenaga, Juan
  • López-Abán, Julio
  • Blanco-Gómez, Adrián
  • Vicente, Belén
  • Burguillo, Francisco J.
  • Pérez-Losada, J.
  • Muro, Antonio
This dataset was intended to describe schistosomiasis severity in a backcross cohort and to study the genetic linkage analysis with parasitological, pathological and immunological variables., [Description of methods used for collection/generation of data] F1BX mice were infected with 150 ± 5 S. mansoni cercariae each mouse and nine weeks post-infection were euthanized. We considered 20 variables: granulomas; affected liver surface (mm2/cm2); the number of adult male and female worms; eggs per gram of liver and small intestine; eggs in liver and small intestine per female; CD4, CD8, CD45, CD220 in blood or spleen; IgG, IgG1, IgG2a, IgM antibodies. Multivariate models (cluster and principal component analyses and K-means) identified four levels of infection intensity in the cohort. The genetic regions associated with severity were assessed by massive genotyping and genetic linkage analysis using 961 informative SNPs., [Methods for processing the data] Mean and standard error in each variable, Kolmogorov-Smirnov test. ANOVA and Tukey’ test or Student t-test. The Pearson correlation coefficient (r) and Student t-test for the statistical significance. Multivariant models considering sex influence in worm recovery, eggs in the liver and intestine, fecundity, granulomas and the affected liver surface. All the variables were standardized to 0 mean and standard deviation to 1. Cluster analysis, dendrogram, Principal components analysis and conglomerates by k-means were used to generate clusters. Median proportions were performed. SIMFIT statistical package for Windows version 7.3.7 were used

Massive genotyping and geneticlinkage analysis using 961 informative SNPs: The genetic distance based on the recombination frequencies between markers in the F1BX cohort was compared with http://cgd.jax.org/mousemapconverter using maximum-likelihood mapping with HM algorithm. The Haldane function was used with a step size of 2.5 cM and a genotyping error of 0.001. We used the LOD-score to calculate the statistical significance of the linkage of the QTLs found. LOD-score higher than 1.4 suggested linkage. The Ensembl bioinformatics tool (https://www.ensembl.org/index.html) was used to identify syntenic regions between mouse and human., Here we present the dataset used in our study entitled "Identification of genomic regions implicated in susceptibility to Schistosoma mansoni infection in a murine genetic model (backcross)". Thus, we crossed the C57BL/6 mouse strain with the CBA one and then the F1B6CBA females (C57 x CBA) were backcrossed with CBA males generating the F1BX cohort of the study. The study consists of the identification of genetic markers of schistosomiasis. High infection levels and severe liver fibrosis in schistosomiasis appear only in a few highly susceptible infected people. Schistosomiasis could be a complex trait disease and it could be possible to identify genetic markers associated with severity. This study uses a genetically heterogeneous back-cross cohort with genetically unique mice. A backcross (F1BX) mouse cohort was generated after two stages; firstly, we crossed a mouse strain (CBA/2J) susceptible to schistosomiasis with a resistant one (C57BL/6J) to generate the F1B6CBA mice; secondly, the F1BX mice were generated by backcrossing. F1B6CBA female mice with CBA/2J males. F1BX mice were infected with 150 ± 5 S. mansoni cercariae each mouse and nine weeks post-infection were euthanized. We considered 20 variables: granulomas; affected liver surface (mm2/cm2); the number of adult male and female worms; eggs per gram of liver and small intestine; eggs in liver and small intestine per female; CD4, CD8, CD45, CD220 in blood or spleen; IgG, IgG1, IgG2a, IgM antibodies. Multivariate models (cluster and principal component analyses and K-means) identified four levels of infection intensity in the cohort. The genetic regions associated with severity were assessed by massive genotyping and genetic linkage analysis using 961 informative SNPs., The main research project is: Red de Investigación Colaborativa en Enfermedades Tropicales (RICET) Ref.: RD16/0027/0018., Peer reviewed




Colorimetric and Real-Time Loop-Mediated Isothermal Amplification (LAMP) for Detection of Loa loa DNA in Human Blood Samples

Repisalud
  • Febrer-Sendra, Begoña
  • Fernández-Soto, Pedro
  • Crego-Vicente, Beatriz
  • García-Bernalt Diego, Juan
  • Ta Tang, Thuy-Huong
  • Berzosa, Pedro
  • Nguema, Rufino
  • Ncogo, Policarpo
  • Romay-Barja, Maria
  • Herrador, Zaida
  • Benito, Agustin
  • Muro, Antonio
Loiasis, caused by the filarial nematode Loa loa, is endemic in Central and West Africa. Loa loa has been associated with severe adverse reactions in high Loa-infected individuals receiving ivermectin during mass drug administration programs for the control of onchocerciasis and lymphatic filariasis. Diagnosis of loiasis still depends on microscopy in blood samples, but this is not effective for large-scale surveys. New diagnostics methods for loiasis are urgently needed. Previously, we developed a colorimetric high-sensitive and species-specific LAMP for Loa loa DNA detection. Here, we evaluate it in a set of 100 field-collected clinical samples stored as dried blood spots. In addition, Loa loa-LAMP was also evaluated in real-time testing and compared with microscopy and a specific PCR/nested PCR. A simple saponin/Chelex-based method was used to extract DNA. Colorimetric and real-time LAMP assays detected more samples with microscopy-confirmed Loa loa and Loa loa/Mansonella perstans mixed infections than PCR/nested-PCR. Samples with the highest Loa loa microfilariae counts were amplified faster in real-time LAMP assays. Our Loa loa-LAMP could be a promising molecular tool for the easy, rapid and accurate screening of patients for loiasis in endemic areas with low-resource settings. The real-time testing (feasible in a handheld device) could be very useful to rule out high-microfilariae loads in infected patients., This research was funded by the Institute of Health Carlos III, ISCIII, Spain (www.isciii.es), grants: RICET RD16/0027/0018 (A.M.), RD16/0027/0000 (A.B.), FCSAI-ISCIII (P.N.) and PI19/01727 (P.F.-S.), European Union co-financing by FEDER (Fondo Europeo de Desarrollo Regional) ‘Una manera de hacer Europa’. We also acknowledge support by the Predoctoral Fellowship Program of Junta de Castilla y León co-financing by Fondo Social Europeo (BDNS (Identif.): 422058 and BDNS (Identif.): 487971), by the ISCIII-Sara Borrell contract CD17CIII/00018 financed by the Institute of Health Carlos III and Predoctoral Fellowship Program of University of Salamanca, and co-financing by Santander Bank., Sí