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Los datos pertenecen al trabajo: Gómez, E.J., Delgado, J.A., González, J.M. (2020) Environmental factors affect the response of microbial extracellular enzyme activity in soils when determined as a funciton of water availability and temperature. Ecology and Evolution (Artícle in press), Figure 2. Extracellular glucosidase activity at 20ºC (A) and 60ºC (B) as a function of water activity for different soils. Enzyme activity is presented as percentage of maximum activity for each soil. Error bars represent the standard deviation. Symbols represent different soils: Black square, Galicia; Grey square, Aragón; Black triangle, Salamanca; Grey triangle, Seville; Black circle and dashed line, Cádiz. Figure 3. Extracellular phosphatase activity at 20ºC (A) and 60ºC (B) as a function of water activity for different soils. Enzyme activity is presented as percentage of maximum activity for each soil. Error bars represent the standard deviation. Symbols represent different soils: Black square, Galicia; Grey square, Aragón; Black triangle, Salamanca; Grey triangle, Seville; Black circle and dashed line, Cádiz. Figure 4. Extracellular protease activity at 20ºC (A) and 60ºC (B) as a function of water activity for different soils. Enzyme activity is presented as percentage of maximum activity for each soil. Error bars represent the standard deviation. Symbols represent different soils: Black square, Galicia; Grey square, Aragón; Black triangle, Salamanca; Grey triangle, Seville; Black circle and dashed line, Cádiz., This study was supported by funding through projects from the Spanish Ministry of Economy and Competitiveness (CGL2014-58762-P) and the Regional Government of Andalusia (RNM2529). These projects have been cofunded by FEDER funds., Peer reviewed

Los datos pertenecen al trabajo: Gómez, E.J., Delgado, J.A., González, J.M. (2020) Environmental factors affect the response of microbial extracellular enzyme activity in soils when determined as a funciton of water availability and temperature. Ecology and Evolution (Artícle in press), RDA plot showing the correspondence of water activity giving the optimum enzyme activity and environmental parameters. Capital letters (in black) represent the sampled soils (G, Galicia, P, Aragón; S, Salamanca; C, Sevilla; T, Cádiz). Arrows represent the environmental variables (soil texture, sand and silt content) contributing significantly to explain the variability of water activity resulting in optimum enzyme activity. The distribution of enzyme activities are presented in red: Glu_20, glucosidase activity at 20ºC; Glu_60, glucosidase activity at 60ºC; Pho_20, phosphatase activity at 20ºC; Pho_60, phosphatase activity at 60ºC; Pro_20, protease activity at 20ºC; Pro_60, protease activity at 60ºC. Figure, This study was supported by funding through projects from the Spanish Ministry of Economy and Competitiveness (CGL2014-58762-P) and the Regional Government of Andalusia (RNM2529). These projects have been cofunded by FEDER funds., Peer reviewed

Los datos pertenecen al trabajo: Gómez, E.J., Delgado, J.A., González, J.M. (2020) Environmental factors affect the response of microbial extracellular enzyme activity in soils when determined as a funciton of water availability and temperature. Ecology and Evolution (Artícle in press), Scattered plots showing the relationship between water activity (X-axis), percentage of maximum enzyme activity estimates at 20ºC (left) and 60ºC (right) (proportional to diameter of circles) with environmental variables (Y-axis), specifically, two climaterelated parameters, the annual average of hot days (>30ºC)(A) and the annual average of consecutive days without precipitation (B), and soil-texture through the fraction of sand in the sampled soils (C). Symbol colors indicate the type of enzyme (Dark to light: Glucosidase, Phosphatase, Protease) and the analyzed soil (Greenish, Galicia (G); bluish, Aragón (P); brownish, Salamanca (S); reddish, Sevilla (C); purplish, Cádiz (T))., This study was supported by funding through projects from the Spanish Ministry of Economy and Competitiveness (CGL2014-58762-P) and the Regional Government of Andalusia (RNM2529). These projects have been cofunded by FEDER funds., Peer reviewed

Los datos pertenecen al trabajo: Gómez, E.J., Delgado, J.A., González, J.M. (2020): Persistence of microbial extracellular enzymes in soils under different temperatures and water availabilities Ecology and Evolution (Artícle in press), Examples of the decay curves for extracellular enzymes from mesophiles and thermophiles at 20ºC and 60ºC in a South Spain (Seville) soil under water activity 1 (wet conditions). Red circles, decay of enzyme activity from thermophiles at 60ºC; pink circles, decay of enzyme activity from thermophiles at 20ºC; black triangles, decay of enzyme activity from mesophiles at 20ºC; grey triangles, decay of enzyme activity from mesophiles at 60ºC. Points are average values from triplicates. Error bars indicate a standard deviation, No

Los datos pertenecen al trabajo: Gómez, E.J., Delgado, J.A., González, J.M. (2020): Persistence of microbial extracellular enzymes in soils under different temperatures and water availabilities Ecology and Evolution (Artícle in press), Figure 2. Decay rates as a function of water availability and temperature for extracellular enzymes from mesophiles and thermophiles at three different soils. Extracellular enzymes: A, B and C (upper row), glucosidases; D, E and F (central row), phosphatases; G, H and I (lower row), proteases. Left column (A, D and G), Seville soil (South Spain); Center column (B, E and H), Cadiz soil (South Spain); Right column (C, F and I), North Spain soil. Symbols: in red, decay of extracellular enzymes from thermophiles at 60ºC; in blue, decay of extracellular enzymes from mesophiles at 20ºC; in black, decay of extracellular enzymes from thermophiles at 20ºC. Points resulted from the average of triplicated samples. Error bars indicate a standard deviation, Figure 3. NMDS ordination of decay rates as a function of temperature, water availability and the soils for glucosidase (A), phosphatase (B) and protease (C) activities. Water activity is shown with brownish filled circles from dark to light in decreasing levels of water activity. Decays at 20ºC and 60ºC by enzymes from mesophilic (M20, down-pointing triangles, and M60, up-pointing triangles, respectively) and thermophilic (T20, squares, and T60, diamonds, respectively) microoorganisms are shown as unfilled symbols for each studied soil (Huesca soil [North Spain] in green, Cadiz soil [South Spain] in blue, Seville soil [South Spain] in red)., No

[UPDATE to version 2 of this upload] Also the raw (no data cleaning applied to it) occurrence dataset as extracted from EFSA DWH is provided in csv format. This dataset is compliant with EFSA SSD model and contains two additional columns documenting issues identified in the cleaning process (column: issue) and the action taken (column: action) to address the issue (e.g. delete record or update values in specific fields). [Description - Version 1] Annex: Tables on GAs on occurrence data in food and feed, and dietary exposure assessment for humans Table A.1. Dietary surveys used for the estimation of acute dietary exposure to GA Table A.2. Number of results and samples per food category submitted to EFSA through the continuous call for data Table A.3. Analytical results excluded from the final dataset used to estimate dietary exposure and the criteria applied for exclusion Table A.4. Occurrence of alpha-chaconine and alpha-solanine (UB mg/kg) in the samples included in the final dataset (left censored results highlighted in yellow) Table A.5. European Starch Association data on feed and potatoes for starch Table A.6. Details acute assessment across surveys (consumption days only) Table A.7. Comparison of exposure summary results obtained using the uniform vs the normal distribution for reduction factors, Peer reviewed

Annex: Summary statistics on occurrence and consumption data and exposure assessment results Table 1 Number of analytical results excluded from the initial dataset during data cleaning, and justification for exclusion Table 2 Occurrence values of OTA (µg/kg) in food as reported in the cleaned database Table 3 Occurrence values of OTA (µg/kg) in food as used for the exposure assessment Table 4 Dietary surveys per country and age group available in the EFSA Comprehensive Database, considered in the exposure assessment Table 5 Results of chronic dietary exposure assessment on OTA (ng/kg bw per day) Table 6 Main contributing food categories to the mean LB exposure assessments to OTA across European dietary surveys and population groups Table 7 Distribution of LOQ values among the different food categories Table 8 All contributing food categories to the mean LB and UB exposure to OTA across European dietary surveys and population groups FormattedDATA_OchratoxinA.zip: Occurrence data on OTA Contains the occurrence data of OTA on 73,891 food samples presented in the opinion as described in its section 4.7 Occurrence data., Peer reviewed

Annex_III_occurrence data.xlsx This Annex is an excel file presenting tables on occurrence data on nitrate and nitrite in feed. Nitrate Nitrite_OCC_ZENODO.CSV Contains the raw (no data cleaning applied to it) occurrence dataset on nitrate and nitrite as extracted from EFSA DWH on 03 December 2019 in feed samples presented in the opinion as described in its section 3.2.2. The data is provided in csv format. This dataset is compliant with EFSA SSD model and contains two additional columns documenting issues identified in the cleaning process (column: issue) and the action taken (column: action) to address the issue (e.g. delete record or update values in specific fields)., Peer reviewed

This Annex is an excel file which presents tables on chlorinated paraffins on occurrence data in food and dietary exposure assessment for humans., Peer reviewed