Resultados de investigación

1 Departamento Biologia Celular, Genetica y Fisiologia, Instituto de Investigacion Biomedica de Malaga y Plataforma en Nanomedicina-IBIMA Plataforma BIONAND, Facultad de Ciencias, Universidad de Malaga, 29071, Malaga, Spain. 2 CIBER de Enfermedades Neurodegenerativas (CIBERNED), Instituto de Salud Carlos III, Spain. 3 Institute for Memory Impairments and Neurological Disorders, University of California, Irvine, CA 92697, USA. 4 Institute of Crystallography, National Research Council (CNR-IC), 95126, Catania, Italy. 5 Department of Neurology, The University of Texas Health Science Center at Houston, Houston, TX, USA. 6 Centro Integrativo de Biologia y Quimica Aplicada. Universidad Bernardo O’Higgins. Santiago, Chile. 7 Department of Neurobiology and Behavior, University of California, Irvine, CA 92697, USA., Este trabajo se centra en el estudio de la propagación de la patología amiloidea en diferentes modelos animales para la Enfermedad de Alzheimer (EA). Los resultados sugieren que existen diversos factores que modulan este proceso de propagación, tales como, el origen de la semilla como los modelos empleados. En nuestro caso, empleamos dos modelos transgénicos de la EA que representan la forma familiar y otro la forma esporádica. Vemos que la propagación en ambos modelos es diferente, siendo mucho más lenta en el modelo esporádico. Así mismo, hemos evaluado en este trabajo como muestras cerebrales de distinto origen (humano y ratón) tienen capacidades diferentes de inducir y propagar la patología amiloidea. Vemos que las muestras humanas tienen mayor potencialidad de inducir la patología amiloidea, respecto a las muestras de roedores. Esto es debido a que el perfil molecular y conformaciones amiloideas es diferente en el extracto cerebral del paciente humano respecto al del modelo roedor. Así mismo, vemos que estos extractos afectan de forma diferencial a las células microgliales, encargadas de la compactación y formación de las placas amiloideas. Estudios de esta naturaleza son esenciales, para determinar que conformaciones amiloideas son las más patogénicas y como afectan y modulan la propagación patológica de la enfermedad. Para la realización de este trabajo se han empleado varios modelos transgénicos para la EA, los modelos 3xTg-AD y hAb-KI. Así mismo, se han empleado técnicas histológicas mediante el uso de inmunohistoquímica y microscopía tanto óptica como fluorescente de confocal, para determinar cambios patogénicos es estos modelos. Además, se han empleado técnicas moleculares como western-blot, dot-blot, proteómica para analizar las conformaciones y agregados amiloideos. Y finalmente, se han empleado cultivos celulares con células BV2 para ver la toxicidad de estos extractos. Los experimentos han sido validados al ser cuantificados por varios investigadores, los datos han sido replicados con una N de entre 5-7 animales, obteniéndose datos similares entre ellos., Los autores declaran haber recibido la siguiente fuente de financiación para la investigación, autoría y/o publicación de este artículo: Beca del Ministerio de Ciencia e Innovación PID2019-108911RA-100 (D.B.V), beca de la Alzheimer’s Association AARG-22-928219 (D.B.V), programa Beatriz Galindo BAGAL18/00052 (D.B.V), beca del Instituto de Salud Carlos III (ISCiii) PI21/00915 (A. G) cofinanciada por fondos FEDER de la Unión Europea; National Institute of Health (NIH) U54-AG054349 (F.M.L); Alzheimer's Association AARGD-22-972125 (R.M.) y National Institutes of Health R01AI132695 (R.M.). Agradecemos el uso de tejidos obtenidos por el National Disease Research Interchange (NDRI) con el apoyo de la subvención U42OD11158 de la NIH.

1) Description of the methods for collection/generation of data: Behavioral data. Inmunohistochemical data. Western blot data. Data on tau levels in plasma and brain homogenates. Statistical analyses. 2) Data processing methods. 3) Software or instruments needed to interpret the data.

Experimental results and the corresponding code used to generate the plots., The provided ZIP file contains a structured dataset organized by figure and table. Each folder includes the corresponding raw data and the scripts required to reproduce or quantify the reported results. Specifically, the file lecturamedidadspNature.m allows the user to plot control variables stored in the .dat files within each folder. Similarly, phase current data stored in .csv format can be visualized using RepresentadorOsciloscopioNature.m. All scripts are implemented in MATLAB and can be executed without modification to replicate the figures presented in the paper., Experimental results and corresponding code used to generate the plots

Link to the questionnaire in Google Forms from which the data is obtained: https://docs.google.com/forms/d/1JoJfv8R0LD9_hU-XVH3qdO16qCONIuNLfUV1Of6Xzks/edit, The database contains the responses of 242 undergraduate students at the University of Malaga to a questionnaire conducted on the concept “Interest in applying Building Information Modeling (BIM) to the visualization of information in seat reservation systems”. This analysis was performed with the aim of demonstrating the interest of using the BIM model of a built space for the purpose of enabling the personalization of reservations in occupancy management systems and to bring virtual knowledge of the building to new users, thus introducing improvements in existing systems., This work was funded by the Vice-rectorate of the Smart-Campus of the University of Malaga within the first Smart-Campus Initiative.

This dataset contains a number of experiments where sensory data is requested from a remote robot through the Internet. Each experiment is a time series of the roundtrip delays measured, which include network, operating systems and application delays. It includes experiments carried out in different geographical locations, with different software architectures and robots, also simulated ones. The range of the parameters that define the experiments (geographical location, amount of data requested, software in the loop, network technologies, etc.) is very broad.

The archive contains the SAS code to analyze the performance of the repeated measures ANOVA with bootstrap procedure in terms of power with non-normal data under violation of sphericity., Ministerio de Ciencia e Innovación, PID2020-113191GB-I00

Erasing memories of cocaine-stimuli associations might have important clinical implications for addiction therapy. Stimulating hippocampal plasticity by enhancing adult hippocampal neurogenesis (AHN) is a promising strategy because the addition of new neurons may not only facilitate new learning but also modify previous connections and weaken retrograde memories. To investigate whether increasing AHN prompted the forgetting of previous contextual cocaine associations, mice trained in a cocaine-induced conditioned place preference (CPP) paradigm were administered chronic intracerebroventricular infusions of lysophosphatidic acid (LPA, an endogenous lysophospholipid with pro-neurogenic actions), ki16425 (an LPA1/3 receptor antagonist) or a vehicle solution, and they were tested 23 days later for CPP retention and extinction. The results of immunohistochemical experiments showed that the LPA-treated mice exhibited reduced long-term CPP retention and an approximately twofold increase in the number of adult-born hippocampal cells that differentiated into mature neurons. Importantly, mediation analyses confirmed a causal role of AHN in reducing CPP maintenance. In contrast, the ki16425-treated mice displayed aberrant responses, with initially decreased CPP retention that progressively increased across the extinction sessions, leading to no effect on AHN. The pharmacological treatments did not affect locomotion or general exploratory or anxiety-like responses. In a second experiment, normal and LPA1 -receptor-deficient mice were acutely infused with LPA, which revealed that LPA1 -mediated signaling was required for LPA-induced proliferative actions. These results suggest that the LPA/LPA1 pathway acts as a potent in vivo modulator of AHN and highlight the potential usefulness of pro-AHN strategies to treat aberrant cognition in those addicted to cocaine., This study was funded by grants from the Spanish Ministry of Economy and Competitiveness (Agencia Estatal de Investigación), which is co-funded by the European Research Development Fund (AEI/FEDER, UE) (PSI2013-44901-P and PSI2017-82604-R to L.J.S. and PSI2015-73156-JIN to E.C.O.); by the National System of Health-Instituto de Salud Carlos III, which is co-funded by AEI/FEDER, UE (Red de Trastornos Adictivos; RD16/0017/0001 to F.R.d.F.); and by the Andalusian R&D&I Programme, Regional Ministry of Economy and Knowledge (PAIDI CTS643 to G.E.T.).

Material complementario del manuscrito.

La base de datos hace referencia al artículo: Martín-Serra A, Nanova O, Varón-González C, Ortega G, Figueirido B. 2019 Phenotypic integration and modularity drives skull shape divergence in the Arctic fox (Vulpes lagopus) from the Commander Islands. Biol. Lett. 15: 20190406. En ella se proporciona las coordenadas de los landmarks digitalizados en los cráneos de la especie Vulpes lagopus.

The aim of the present investigation was to analyze the psychometric properties of the Spanish version of the Teacher Emotionally Intelligent Behavior Scale (TEIBS-S) in two independent studies with secondary students., Parte del proyecto I+D+i PID2020-117006RB-I00, financiado por MCIN/AEI/10.13039/501100011033, Grupo de investigación Applied Positive Lab CTS-1048G-FEDER (Junta de Andalucía)