Resultados de investigación

[EN] Ontological model that categorises dimensions, domains, and types of problems that can affect citizens' quality of life and well-being., Esta ontología es parte del proyecto de I+D+i PID2020-112827GB-I00 financiado por MICIU/AEI/10.13039/501100011033.

[EN] The overall objective of the NEW4ALMOND project is to improve the health status and productivity of almond, through the development of techniques and procedures that allow reducing the impact of the most important diseases of the crop and thus contribute to its management from an economic and environmental point of view. The NEW4ALMOND_SP3_Diaporthe_amygdali_Control dataset includes part of the data obtained in the activities of Subproject 3 developed at the UPV related with improving the knowledge about potential strategies for Diaporthe dieback management.

[EN] This research work studies the development of an electrochemical cell prototype based on textile electrodes for treating wastewater via electrochemical processes. The present prototype proposes a system that offers greater efficiency regarding the size/surface of electrodes in electrolysis processes. This prototype enables the treatment of large volumes of dissolution. Moreover, this prototype could surface modify textile electrodes by an electrochemical process in the same cell, specifically, an electrochemically reduced graphene oxide intermediate layer and an outer layer of electrochemically reduced metal nanoparticles are proposed. Surface modification by reduced graphene oxide increase the textile electrode’s stability, conductivity, and specific surface, whilst Pt nanoparticles increase electroactivity. Amaranth was selected to validate the use of this prototype in treating emerging pollutants. This is an azoic dye with a simple structure. Various analytical techniques demonstrate that colour removal takes place with an electrical energy consumption of between 0.29 and 4.66 kWh m−3 (depending on the operational specifications of the electrolysis performed). Once the colour is removed, total organic carbon and chemical oxygen demand decreases by up to 49% and 37%, respectively., This research is part of the Spanish R+D+i contract (TED2021-130905B-I00 funded by MCIN/ AEI/10.13039/501100011033 and the “European Union NextGenerationEU/PRTR”). The authors wish to acknowledge the Spanish AEI Research Network (Contract RED2022-134552-T). Chemviron Carbon, who kindly donated the FlexzorbTM FM10 activated carbon fabrics and Funding for open access charge CRUE-Universitat Politècnica de València, are acknowledged too. The authors are grateful for the English revision by John Rawlins and Tim Vickers.

The early intestinal response of Mugil cephalus to fishmeal replacement with alternative protein sources under biofloc technology (BFT) was assessed. Juvenile mullet (82.68 ± 0.05 g) were distributed among three dietary treatments with decreasing fishmeal inclusion levels of 15%, 5%, and 0%, corresponding to FM15, FM5, and FM0, respectively. After 27 days of feeding, intestinal samples were analyzed for microbiota composition, transcriptomic responses and enzymatic activity. The FM0 group exhibited reduced intestinal enzyme activity, whereas no significant differences were observed in terms of gut microbiota among the diets. Furthermore, transcriptomic analysis revealed differentially expressed genes associated with enriched GO terms and KEGG pathways related to lipid metabolism. This response was consistent with the lower mesenteric fat indices and whole-body lipid content observed in fish fed the FM0 diet, suggesting an early metabolic adjustment associated with complete fishmeal replacement and the higher dietary protein content. All diets exhibited high protein efficiency ratios (9.98 ± 2.42) and low feed conversion ratios (0.40 ± 0.17), with no significant differences observed among them. However, complete fishmeal replacement negatively affected intestinal physiology and lipid storage, indicating that these alterations were not fully compensated for by the biofloc system and may not be physiologically sustainable over longer periods. Further studies over an extended period could be carried out to evaluate the long-term effects of these diets., This work was supported by Grant TED2021-129272B-C21, funded by MICIU/AEI/10.13039/501100011033, and by the European Union NextGenerationEU/PRTR and the Generalitat Valenciana (GVA AICO/2021/198). J. Brol has a predoctoral grant from Generalitat Valenciana (Programa Santiago Grisolía 2021; CIGRIS/2021/109). R. Olivares-Perona has a technician contract that was funded by TED2021-129272B-C21. Funding for open access charge: Universitat Politècnica de València.

Background and Objective: Assessment of drug cardiotoxicity is critical in the development of new compounds and modeling of drug-binding dynamics to hERG can improve early cardiotoxicity assessment. We previously developed a methodology to generate Markovian models reproducing preferential state-dependent binding properties, trapping dynamics and the onset of IKr block using simple voltage clamp protocols. Here, we test this methodology with real IKr blockers and investigate the impact of drug dynamics on action potential prolongation. Methods: Experiments were performed on HEK cells stably transfected with hERG and using the Nanion SyncroPatch 384i. Three protocols, P-80, P0 and P40, were applied to obtain the experimental data from the drugs and the Markovian models were generated using our pipeline. The corresponding static models were also generated and a modified version of the O´Hara-Rudy action potential model was used to simulate the action potential duration. Results: The experimental Hill plots and the onset of IKr block of ten compounds were obtained using our voltage clamp protocols and the models generated successfully mimicked these experimental data, unlike the CiPA dynamic models. Marked differences in APD prolongation were observed when drug effects were simulated using the dynamic models and the static models. Conclusions: These new dynamic models of ten well-known IKr blockers constitute a validation of our methodology to model dynamic drug–hERG channel interactions and highlight the importance of state-dependent binding, trapping dynamics and the time-course of IKr block to assess drug effects even at the steady-state.

[EN] The benchmarking of security tools is endeavored to determine which tools are more suitable to detect system vulnerabilities or intrusions. The analysis process is usually oversimplified by employing just a single metric out of the large set of those available. Accordingly, the decision may be biased by not considering relevant information provided by neglected metrics. This work proposes a novel approach to take into account several metrics, different scenarios, and the advice of multiple experts. The proposal relies on experts quantifying the relative importance of each pair of metrics towards the requirements of a given scenario. Their judgments are aggregated using group decision making techniques, and pondered according to the familiarity of experts with the metrics and scenario, to compute a set of weights accounting for the relative importance of each metric. Then, weight-based multi-criteria-decision-making techniques can be used to rank the benchmarked tools. This dataset contains raw data obtained from 21 experts, who declared their familiarity with considered metrics and their preference for each metric in the considered scenarios. Processed data include the consistency ratio of resulting pairwise comparison matrices so inconsistent matrices are rejected - weight = 0.00), the relative contribution of each expert according to their declared familiarity with metrics and computed CRs, and the contribution (weight) of each metric towards each considered scenario.

Alginate hydrogels can be used to develop a three-dimensional environment in which various cell types can be grown. Cross-linking the alginate chains using reversible ionic bonds opens up great possibilities for the encapsulation and subsequent release of cells or drugs. However, al-ginate also has a drawback in that its structure is not very stable in a culture medium with cellu-lar activity. This work explored the stability of alginate microspheres functionalised by grafting specific biomolecules onto their surface to form microgels in which biomimetic microspheres surrounded the cells in the culture, reproducing the natural microenvironment. A study was made of the stability of the microgel in different typical culture media and the formation of polyelectrolyte multilayers containing polylysine and heparin. Multiple myeloma cell prolifer-ation in the culture was tested in a bioreactor under gentle agitation., The Microscopy Service of the UPV (Universitat Politècnica de València) is gratefully acknowl-edged for helping with cryoFESEM characterisation. CIBER-BBN is an initiative funded by the VI National R&D&I Plan 2008–2011, Iniciativa Ingenio 2010, Consolider Program. The Instituto de Salud Carlos III financed CIBER Actions with assistance from the European Regional Develop-ment Fund.

Base de datos de los museos marítimos españoles con datos sobre fondos recibidos, This research was funded by SPANISH ECONOMY AND COMPETITIVENESS MINISTRY, grant number PID2019-105497 GB-I00, European Fisheries Funds, opportunities for the fisheries sector through diversification, and the FLAGs management (DivPesc).

[EN] Dataset asociated to the paper "Above and belowground male population of the Invasive Cit-rus mealybug Delottococcus aberiae De Lotto (Hemiptera: Pseudococcidae) " The invasive Delottococcus aberiae is one of the most harmful mealybugs on citrus in Spain, since it causes deformations and the premature falling of fruits. To improve control strategies, this study evaluates its population above and belowground, distri-bution in the soil and identify natural enemies emerging from belowground of citrus orchards. The distribution in the soil of emerged males varies significantly with the distance to the tree trunks with an annual average number of 0.95 males/trap/day at 0.5 m, and ranging between 0.25-0.32 at 1 m, 1.5 m and 2 m. There is a constant emergence of males throughout the year, both aboveground and belowground, with four distinct peaks, the first two occurring in February and April. This observation is essential to manage fruit damages that follows between March and June. Among the natural en-emies detected belowground the following are highlighted: Bdellidae mites (52%), Hymenoptera parasitoids (16%) and spiders (11%). When implementing IPM strate-gies, emphasis should be placed on adequate soil management (crops, mulching, or the release of natural enemies) and to the appearance of first generation. We propose a belowground population sampling method as a simple and practical tool to quantify this pest’s the winter and spring populations, which remain undetectable using current methodologies., This research was funded by the Next Generation European Union and the Plan de Recuperación, Transformación y Resiliencia of the Spanish Government, with the support of Generalitat Valenciana (project AGROALNEXT/2022/043), and by the project “Ruralfruit-AgroEco” (Transición al modelo agroecológico y adaptación al cambio climático de la citricultura convencional en Cooperativas valencianas” (project AGCOOP_A/2022/015), subsidized by the PDR-CV 2014-2020 (53% UE, 8,86% MAPA, 38,14% GVA)

[EN] This dataset includes supplementary material (raw data) collected to support research on patent-level altmetrics on X (Twitter). Specifically, it contains the linking tweets to patent US4656917A, an invention owned by musician Eddie Van Halen, all conversations in which those tweets appear, as well as information on the Twitter activity of the users publishing those tweets and the users interacting (like or retweet) with those tweets., This research was funded by MCIN/AEI/ 10.13039/501100011033 project PID2022-142569NA-I00 and by “ERDF A way of making Europe”, by the “European Union”.