Resultados de investigación

Peer reviewed

Dopamine replacement therapy for Parkinson's disease (PD) may lead to disabling incontrollable movements known as L-DOPA-induced dyskinesias. Transcranial magnetic stimulation (TMS) has been applied as non-invasive therapy to ameliorate motor symptoms and dyskinesias in PD treatment. Recent studies have shown that TMS-induced motor effects might be related to dopaminergic system modulation. However, the mechanisms underlying these effects of TMS are not fully understood., This research was supported by fideicomiso “Bases de colaboración: transplantes cerebrales” to RDC and Facultad de Medicina, UNAM to GRR. FRL was a doctoral student from Programa de Doctorado en Ciencias Biomédicas, Universidad Nacional Autónoma de México (UNAM) and had a CONACyT doctoral fellowship (295881)., Peer reviewed

Data Sheet 1 and table 1, The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fpls.2024.1399072/full#supplementary-material, This research was funded by the project AGL2016-77628-R, Spain; and IN 607A – Grupos de referencia competitiva (GRC) Xunta de Galicia, Spain., Data Sheet 1: Supplementary Figure 1,2,3,4 and 5; Table 1: Supplementary Table 1, 2,3 and 4., Peer reviewed

This excel data set contains the electrophysiological data presented in the paper including figure 1I, 1J, figure 3, figure 5, suppl. figure 2A, suppl. figure 3, suppl. figure 6E, 6G, 6L & 6N., Swedish Research Council, Peer reviewed

The files contain the data included in Figure 2B, Figure 2C, Figure 4B, Figure 4C, Figure 6B, Figure 6C, Suppl.Fig.4, Suppl. Figure 5, Suppl. Figure 6I, Suppl. Figure 6J., Peer reviewed

The files contain the original raw blot (Figure 3—figure supplement 1-source data 1) and the raw blot with labeling (Figure 3—figure supplement 1-source data 2)., Peer reviewed

Significant progress has been made with regard to understanding how the adult brain responds after a stroke. However, a large number of patients continue to suffer lifelong disabilities without adequate treatment. In the present study, we have analyzed possible microanatomical alterations in the contralesional hippocampus from the ischemic stroke mouse model tMCAo 12-14 weeks after transient middle cerebral artery occlusion. After individually injecting Lucifer yellow into pyramidal neurons from the CA1 field of the hippocampus, we performed a detailed three-dimensional analysis of the neuronal complexity, dendritic spine density, and morphology. We found that, in both apical (stratum radiatum) and basal (stratum oriens) arbors, CA1 pyramidal neurons in the contralesional hippocampus of tMCAo mice have a significantly higher neuronal complexity, as well as reduced spine density and alterations in spine volume and spine length. Our results show that when the ipsilateral hippocampus is dramatically damaged, the contralesional hippocampus exhibits several statistically significant selective alterations. However, these alterations are not as significant as expected, which may help to explain the recovery of hippocampal function after stroke. Further anatomical and physiological studies are necessary to better understand the modifications in the "intact" contralesional lesioned brain regions, which are probably fundamental to recover functions after stroke., CB06/05/0066/Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas 390857198/Deutsche Forschungsgemeinschaft PCI2018-092874/ERA-NET NEURON IJCI-2016-27658/MCIN/AEI/10.13039/501100011033 PID2021-127924NB-I00/MCIN/AEI/10.13039/501100011033 FPU19/00007/Spanish Ministry of Universities, Peer reviewed

All LC-Qtof MS files in mzXML format have been converted to two archive files (positive and negative ionization modes). The addresses of links: Positive mode ionization (https://drive.google.com/file/d/1RXcMzDDkRy7QCCx_UrtK93FZq-PGmkQv/view?usp=drive_link). Negative mode ionization (https://drive.google.com/file/d/1Pz_yC6pBFnmt23qo3UksYRp4Ph-W8gKc/view?usp=drive_link)., Z. Amjadi and H. Hamzehzarghani acknowledge the financial support of Shiraz University. YJ. Huang acknowledges financial support from the Department for Environment, Food and Rural Affairs (OREGIN, CH0110). V.M. Rodriguez acknowledges financial support from the Group of Genetics, Breeding, and Biochemistry of Brassicas., Peer reviewed

The assembly of the gut resistome in early life is key to infant health. Specific perinatal factors such as cesarean section (C-section), antibiotic exposure and lack of breastfeeding practices are detrimental to proper microbial development and increase the antimicrobial resistance genes (ARGs). Using 265 gut longitudinal metagenomes from 66 mother-infant pairs, we investigated how perinatal factors influence the acquisition and dynamics of ARGs during the first year of life. Our findings reveal that Bifidobacterium plays a crucial role in modulating the infant resistome, with its high relative abundance being associated with a lower ARG load. Exclusive breastfeeding during the first month of life accelerates the reduction of ARGs and ensures a lower resistome burden at six months. Moreover, early breastfeeding cessation correlates with a higher ARG load, underscoring its long-term influence on microbial resilience. Importantly, we identify exclusive breastfeeding as a key strategy to mitigate the impact of C-section delivery on the infant gut resistome, counteracting the early-life antibiotic exposure associated with this procedure and the resulting resistance acquisition. By promoting a microbiome enriched in Bifidobacterium, breastfeeding may help suppress ARG-carrying taxa, reducing the risk of resistance dissemination. Our findings underscore the importance of breastfeeding as a natural intervention to shape the infant microbiome and resistome. Supporting breastfeeding through public health policies could help limit the spread of antimicrobial resistance in early life., We acknowledge the Generalitat Valenciana-European Social Fund (ACIF/2021) for the predoctoral fellowship grant to A.S. M.C.C. acknowledges the support from H2020-ERC Starting Grant (MAMI- ref. 639226), EU Horizon Europe Programme grant (INITIALISE ref. 101094099), Spanish Ministry of Science and Innovation (MCIN) research grant (MAMI plus, ref. PID2022-139475OB-I00), and also, Excellence Research Group-Generalitat Valenciana (GVA) Prometeo Programme (NeoHealth ref. PROMETEO2020/12 & Microglocal ref. CIPROM2023/030). NMQ is currently funded by the Generation D initiative, promoted by Red.es, an organisation attached to the Ministry for Digital Transformation and the Civil Service, for the attraction and retention of talent through grants and training contracts, financed by the Recovery, Transformation and Resilience Plan through the European Union's Next Generation funds. IATA‐CSIC authors would like to acknowledge the award of the Spanish government MCIN/AEI to the IATA‐CSIC as Center of Excellence Accreditation Severo Ochoa (CEX2021‐001189‐S/MCIN/AEI/10.13039/501100011033)., With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2021-001189-S), Peer reviewed

This research was funded by the Spanish Ministry of Science and Innovation/FEDER funds Grant PID2020-116261GB-I00/AEI/10.13039/501100011033 (JAG), by the European Regional Development Fund Andalucía 2014–2020 Grant UAL18-CTS-B032-A (FRV) and by the Own Research and Transfer Plan 2020 of the University of Almeria Grant PPUENTE2020/006 (FJLHV). European Synchrotron Radiation Facility (ESRF), Grenoble, France, proposal MX2353ESRF, Peer reviewed